1 research outputs found
Typing of Blood-Group Antigens on Neutral Oligosaccharides by Negative-Ion Electrospray Ionization Tandem Mass Spectrometry
Blood-group antigens, such as those
containing fucose and bearing
the ABOÂ(H)- and Lewis-type determinants expressed on the carbohydrate
chains of glycoproteins and glycolipids, and also on unconjugated
free oligosaccharides in human milk and other secretions, are associated
with various biological functions. We have previously shown the utility
of negative-ion electrospay ionization tandem mass spectrometry with
collision-induced dissociation (ESI-CID-MS/MS) for typing of Lewis
(Le) determinants, for example, Le<sup>a</sup>, Le<sup>x</sup>, Le<sup>b</sup>, and Le<sup>y</sup> on neutral and sialylated oligosaccharide
chains. In the present report, we extended the strategy to characterization
of blood-group A-, B-, and H-determinants on type 1 and type 2 and
also on type 4 globoside chains to provide a high sensitivity method
for typing of all the major blood-group antigens, including the A,
B, H, Le<sup>a</sup>, Le<sup>x</sup>, Le<sup>b</sup>, and Le<sup>y</sup> determinants, present in oligosaccharides. Using the principles
established, we identified two minor unknown oligosaccharide components
present in the products of enzymatic synthesis by bacterial fermentation.
We also demonstrated that the unique fragmentations derived from the
D- and <sup>0,2</sup>A-type cleavages observed in ESI-CID-MS/MS, which
are important for assigning blood-group and chain types, only occur
under the negative-ion conditions for reducing sugars but not for
reduced alditols or under positive-ion conditions