66 research outputs found

    Autophagy genes promote apoptotic cell corpse clearance

    No full text
    Autophagy is a catabolic process through which damaged organelles and protein aggregates are delivered to lysosomes for degradation. Autophagy genes are reported to promote exposure of "eat me" signals on the surface of apoptotic cells, but whether they function in engulfing cells is not clear. Recently, we found that the autophagy mutants atg-18 and epg-5 are defective in removing apoptotic cells derived from the C. elegans Q neuroblast, a phenotype that can be fully rescued by expression of ATG-18 and EPG-5 in the engulfing cell. Loss of ATG-18 or EPG-5 does not affect cell corpse engulfment but causes defects in phagosomal recruitment of RAB-5 and RAB-7 and formation of phagolysosomes. EPG-5, ATG-18 and LGG-1 are sequentially recruited to phagosomes, suggesting that they function at different steps of phagosomal maturation. Our studies indicate that autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell

    Electrolyte-Gated Indium Oxide Thin Film Transistor Based Biosensor With Low Operation Voltage

    No full text

    Facile fabrication of hollow molecularly imprinted polymer microspheres via pickering emulsion polymerization stabilized with TiO2 nanoparticles

    No full text
    The polymer microspheres with hollow structure are of technological importance in the removal of pollutants because of the large specific surface area and low mass transfer resistance. In this work, we employ the Pickering emulsion polymerization stabilized with TiO2 nanoparticles to build the molecularly imprinted polymers microspheres with hollow structure (H-MIPs) for the precise recognition of dibutyl phthalate (DBP), which would influence the biological reproductive system and endocrine system as a typical endocrine disrupting compound. The polymerization induced phase separation occurring within Pickering emulsion droplets plays a significant role in the formation of hollow structure. The morphology and structure of H-MIPs were observed via optical microscope and field emission scanning electron microscope, respectively. The results indicate that H-MIPs microspheres are of irregular spherical form with sunken surface and perfect hollow structure. The forming process of the hollow structure was analyzed in detail. Besides, the chemical structure and thermal stability of H-MIPs and H-NIPs were characterized by fourier transform infrared spectroscopy and thermal gravimetric analysis, respectively. The binding performance of H-MIPs and H-NIPs was investigated through a series of binding experiments, which consist of the binding kinetics, binding isotherm, selective test and reusability experiment. The results show that H-MIPs microspheres exhibit remarkable binding kinetics towards DBP, and the saturated adsorption time is not more than 30 min. Apart from the fast binding rate, H-MIPs also show considerable binding amount, accurate binding selectivity and good regenerability. The imprinting factor could reach to 2.56, and the binding amount towards DBP remains at a relatively high level within 3 cycles. The prepared H-MIPs microspheres have broad application prospects in environmental and analytical field involved DBP, and the study also offers an alternative method for the facile building of H-MIPs microspheres

    Research on the Tolerance and Degradation of o-Cresol by Microalgae

    No full text
    o-Cresol shows high toxicity and stability. To explore a better degradation method of o-cresol, the degradation of o-cresol by three kinds of microalgae (Selenastrum capricornutum, Scenedesmus obliquus, and Microcystis aeruginosa) was researched in this paper. The growth status and degradation rate were used to reflect the tolerance and degradation effect of microalgae. The effects of the medium’s initial pH, microalgal density, and different exogenous pollutants on the degradation of o-cresol by Selenastrum capricornutum were investigated. The results showed that Selenastrum capricornutum had the best degradation effect on o-cresol. microalgal density increased after adaptation to different concentrations of o-cresol for some time. At pH 7.0 as the initial condition, the microalgal exhibited the best results of degradation. When the microalgal density OD680 was 0.20, o-cresol was the first to be completely degraded within 5 days. At higher initial concentrations of o-cresol, the microalgae preferentially degraded glucose to promote the growth of the microalgae under mixotrophic cultivation. Selenastrum capricornutum could degrade phenol and o-cresol at the same time, and the degradation was completed within 8 days when the initial concentration of o-cresol and phenol were 100 and 120 mg/L. It was proven that the degradation of o-cresol by Selenastrum capricornutum is feasible under suitable conditions

    Study on provincial decomposition method of total carbon emission control target based on greenhouse gas inventory

    No full text
    Realizing the transformation from “dual control of energy consumption” to “dual control of carbon emissions” is the inherent requirement of realizing the “dual carbon” goal. Scientific planning of the provincial decomposition method of total carbon emissions is an important content of the top-level design of carbon emissions management, and an important support for the implementation of total carbon emissions control. Based on the application research of greenhouse gas inventory in all fields, this paper conducts a full factor analysis of the total carbon emissions of the whole society, establishes a comprehensive decomposition index system, and proposes a simple and transparent provincial decomposition method of the total carbon emissions control target, which considers fairness and efficiency. The simulated decomposition results show that the decomposition method is scientific and operable and can be used as an important reference for future practical decomposition operations

    Vertical Diamond p-n Junction Diode with Step Edge Termination Structure Designed by Simulation

    No full text
    In this paper, diamond-based vertical p-n junction diodes with step edge termination are investigated using a Silvaco simulation (Version 5.0.10.R). Compared with the conventional p-n junction diode without termination, the step edge termination shows weak influences on the forward characteristics and helps to suppress the electric field crowding. However, the breakdown voltage of the diode with simple step edge termination is still lower than that of the ideal parallel-plane one. To further enhance the breakdown voltage, we combine a p-n junction-based junction termination extension on the step edge termination. After optimizing the structure parameters of the device, the depletion regions formed by the junction termination extension overlap with that of the p-n junction on the top mesa, resulting in a more uniform electric field distribution and higher device performance

    Desalination Characteristics of Cellulose Acetate FO Membrane Incorporated with ZIF-8 Nanoparticles

    No full text
    Forward osmosis membranes have a wide range of applications in the field of water treatment. However, the application of seawater desalination is restricted, so the research of forward osmosis membranes for seawater desalination poses new challenges. In this study, zeolitic imidazolate framework-8 (ZIF-8) was synthesized by a mechanical stirring method, and its crystal structure, surface morphology, functional group characteristics, thermochemical stability, pore size distribution and specific surface area were analyzed. The cellulose acetate (CA)/ZIF-8 mixed matrix forward osmosis membrane was prepared by using the synthesized ZIF-8 as a modified additive. The effects of the additive ZIF-8 content, coagulation bath temperature, mixing temperature and heat treatment temperature on the properties of the CA/ZIF-8 forward osmosis membrane were systematically studied, and the causes were analyzed to determine the best membrane preparation parameters. The structure of the CA membrane and CA/ZIF-8 mixed matrix forward osmosis membranes prepared under the optimal conditions were characterized by Fourier Transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), contact angle and Atomic force microscope (AFM). Finally, the properties of the HTI membrane (Membrane manufactured by Hydration Technology Innovations Inc.), CA forward osmosis membrane and CA/ZIF-8 mixed matrix forward osmosis membrane were compared under laboratory conditions. For the CA membrane, the water flux and reverse salt flux reached 48.85 L·m−2·h−1 and 3.4 g·m−2·h−1, respectively. The reverse salt flux and water flux of the CA/ZIF-8 membrane are 2.84 g·m−2·h−1 and 50.14 L·m−2·h−1, respectively. ZIF-8 has a promising application in seawater desalination
    • …
    corecore