Wireless capsule endoscopy exploration for diseases of the small intestine in China

For small bowel diseases, it is difficult for the ordinary enteroscopy to reach due to its specific curvature and length. Capsule endoscopy (CE) is a unique tool to visualize the mucosa of the small intestine. The aim of this study was to evaluate the detection rate and diagnostic yield of CE in a large group of patients with suspected digestive diseases in China. One hundred and two consecutive patients (75 male, mean age 50 years, range 32-87 years) underwent CE in our Gastroenterology Units, for a total of 102 procedures. Referrals were obscure occult/ overt gastrointestinal bleeding group (19 patients) and suspected small bowel disease group (83). In our study, the whole detection rate was 92 % (94/102), with a definite diagnosis yield of 63 % of the patients in the obscure gastrointestinal bleeding and 39 % of the patients in the suspected small bowel diseases. None of the patients developed symptoms of signs of mechanical obstruction, although the capsule was retained in the stomach in 2/102 patients for their somatostatin taken. CE seems to be a very safe, painless and effective procedure with a high diagnostic yield. Accurate selection of indications and critical evaluation of the results are essential to explore these diseases

Time-optimal trajectory planning based on event-trigger and conditional proportional control.

Trajectory planning is an important issue for manipulators and robots. To get a optimal trajectory, many constraints including actuators specifications, motion range of joints, workspace limitations, etc, and many objectives including the shortest time, the shortest distance, the lowest energy consumption, the minimum oscillations, obstacles-avoiding, etc, should be considered both. In this paper, firstly, the forward kinematics and inverse kinematics of a five axis manipulator are deduced. And, a simple method to choose one appropriate solution from multi solutions of inverse kinematics is proposed. Secondly, an easy-implemented optimization method of trajectory planning is proposed based on seventh order polynomial interpolation, event-trigger mechanism and conditional proportional control (P control). The proposed optimization method can capture the time optimal trajectory, and the actuators specifications including velocity, acceleration of motor can be guaranteed as well. Thirdly, comparative simulations and experiments validate the effectiveness and efficiency of proposed optimization method. The research provides an insight for the application of trajectory optimization on the micro controller with low computing capability and high real-time performance requirement

Design, Preparation and In Vitro Evaluation of Core–Shell Fused Deposition Modelling 3D-Printed Verapamil Hydrochloride Pulsatile Tablets

The aim of the study was to investigate core–shell pulsatile tablets by combining the advantages of FDM 3D printing and traditional pharmaceutical technology, which are suitable for a patient’s individual medication and chronopathology. The tablets were designed and prepared with the commercial verapamil hydrochloride tablets as core inside and the fused deposition modelling (FDM) 3D-printed shell outside. Filaments composed of hydroxypropylmethyl cellulose (HPMC) and polyethylenglycol (PEG) 400 were prepared by hot melt extrusion (HME) and used for fabrication of the shell. Seven types of printed shells were designed for the tablets by adjusting the filament composition, geometric structure and thickness of the shell. A series of evaluations were then performed on the 3D-printed core–shell tablets, including the morphology, weight, hardness, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), in vitro drug release and CT imaging. The results showed that the tablets prepared by FDM 3D printing appeared intact without any defects. All the excipients of the tablet shells were thermally stable during the extruding and printing process. The weight, hardness and in vitro drug release of the tablets were affected by the filament composition, geometric structure and thickness of the shell. The pulsatile tablets achieved personalized lag time ranging from 4 h to 8 h in the drug release test in phosphate-buffered solution (pH 6.8). Therefore, the 3D-printed core–shell pulsatile tablets in this study presented good potential in personalized administration, thereby improving the therapeutic effects of the drug for circadian rhythm disease

Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases

Sesquiterpenes are important defense and signal molecules for plants to adapt to the environment, cope with stress, and communicate with the outside world, and their evolutionary history is closely related to physiological functions. In this study, the information of plant sesquiterpene synthases (STSs) with identified functions were collected and sorted to form a dataset containing about 500 members. The phylogeny of spermatophyte functional STSs was constructed based on the structural comparative analysis to reveal the sequence–structure–function relationships. We propose the evolutionary history of plant sesquiterpene skeletons, from chain structure to small rings, followed by large rings for the first time and put forward a more detailed function-driven hypothesis. Then, the evolutionary origins and history of spermatophyte STSs are also discussed. In addition, three newly identified STSs CaSTS2, CaSTS3, and CaSTS4 were analyzed in this functional evolutionary system, and their germacrene D products were consistent with the functional prediction. This demonstrates an application of the structure-based phylogeny in predicting STS function. This work will help us to understand evolutionary patterns and dynamics of plant sesquiterpenes and STSs and screen or design STSs with specific product profiles as functional elements for synthetic biology application

FDM 3D-Printed Sustained-Release Gastric-Floating Verapamil Hydrochloride Formulations with Cylinder, Capsule and Hemisphere Shapes, and Low Infill Percentage

The aim of this work was to design and fabricate fused deposition modeling (FDM) 3D-printed sustained-release gastric-floating formulations with different shapes (cylinder, capsule and hemisphere) and infill percentages (0% and 15%), and to investigate the influence of shape and infill percentage on the properties of the printed formulations. Drug-loaded filaments containing HPMC, Soluplus® and verapamil hydrochloride were prepared via hot-melt extrusion (HME) and then used to print the following gastric-floating formulations: cylinder-15, capsule-0, capsule-15, hemisphere-0 and hemisphere-15. The morphology of the filaments and the printed formulations were observed by scanning electron microscopy (SEM). The physical state of the drugs in the filaments and the printed formulations were characterized by X-ray diffraction (XRD), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The printed formulations were evaluated in vitro, including the weight variation, hardness, floating time, drug content and drug release. The results showed that the drug-loaded filament prepared was successful in printing the gastric floating formulations. Verapamil hydrochloride was proved thermally stable during HME and FDM, and in an amorphous state in the filament and the printed formulations. The shape and infill percentage of the printed formulations effected the hardness, floating time and in vitro drug release

Urea-mediated dissociation alleviate the false-positive Treponema pallidum-specific antibodies detected by ELISA.

The serological detection of antibodies to Treponema pallidum is essential to the diagnosis of syphilis. However, for the presence of cross-reaction, the specific antibody tests [e.g., enzyme-linked immunosorbent assay (ELISA)] always have false-positive results. In this study, we derived and validated the dissociation of urea in an attempt to alleviate the situation of false-positive antibodies to T. pallidum detected by ELISA. Six serum samples that were false-positive antibodies to T. pallidum detected by ELISA, and 16 control serum samples (8 sera positive for both specific IgG and IgM, and 8 IgG-positive and IgM-negative sera) were collected to select the appropriate dissociated concentration and time of urea. Our goal was to establish improved an ELISA method based on the original detection system of ELISA. The sensitivity of the improved ELISA was evaluated by 275 serum samples with class IgM-positive antibodies to T. pallidum. At 6 mol/L with 10 minutes dissociation of urea, 6 samples with false-positive antibodies to T. pallidum were converted to negative, and compared with true-positive antibodies to T. pallidum. The sensitivity of the improved ELISA was 100% by detecting the class IgM-positive antibodies to T. pallidum in sera of patients with syphilis. Considering the importance at the diagnosis of syphilis, antibodies to T. pallidum in serum samples should be retested by the improved ELISA method to avoid false-positive results

The trajectory planning results of Simulation 3 based on fifth order polynomial interpolation.

(a) Planning position, (b) Planning attitude, (c) Joint angle, (d) Joint angular velocity, (e) Joint angular acceleration, (f) Joint angular jerk.</p

The trajectory planning results of experiment based on the proposed optimization method.

(a) Planning position, (b) Planning attitude, (c) Joint angle, (d) Joint angular velocity, (e) Joint angular acceleration, (f) Joint angular jerk.</p

DH parameters of manipulator.

DH parameters of manipulator.</p