Chinese migrant workers and occupational injuries: A case study of the manufacturing industry in the Pearl River Delta

This paper focuses on the causes and impacts of work-related injuries experienced by migrant workers from manufacturing enterprises in the Pearl River Delta (PRD), a major centre of foreign-invested export industry in China. The paper starts with a brief review of the occupational health and safety legislation in China. The authors analyse a database comprising more than 10,000 cases of injured migrant workers in hospitals in the PRD and in-depth interviews undertaken with injured migrant workers to explore the following questions: how do migrant workers who became victims of occupational accidents describe the conditions, causes and impacts of their work-related injuries? What does this body of knowledge tell us about the gap between the legal situation of occupational health in China and the practical situation of occupational injuries as experienced by migrant workers in the PRD? And how can this knowledge contribute to identifying areas of concern for researchers, policy makers and practitioners? Key findings include the types, frequencies, severity and causes of migrants´ work-related injuries; the current state of occupational safety and preventive measures; medical treatment and related costs (including a discussion of whether migrants tend to return home after these injuries); and health care services, health insurance and compensation for injured migrant workers. The paper concludes with recommendations for further research as well as for policy and programme interventions to prevent risk and promote protection within the broader context of institutional adaptation in the Chinese health system

Inducible Enrichment of Osa-miR1432 Confers Rice Bacterial Blight Resistance through Suppressing OsCaML2

MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and Xanthomonas oryzae pv. oryzae (Xoo) are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under Xoo strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to Xoo, indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to Xoo infection. Noticeably, OsCaML2 (LOC_Os03g59770) was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing OsCaML2 transgenic plants exhibited compromised resistance to Xoo infestation. Our results indicate that osa-miR1432 and OsCaML2 were differently responsive to Xoo invasion at the transcriptional level and fine-tune rice resistance to Xoo infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving Xoo resistance in rice breeding

Natural gas development prospect in Changqing gas province of the Ordos Basin

At present, natural gas accounts for a low proportion in China's primary energy consumption structure and is highly dependent on foreign sources. Changqing gas province of the Ordos Basin where PetroChina Changqing Oilfield Company (referred to as Changqing Oilfield) is located is one of China's four major natural gas production bases. It bears the important responsibility for ensuring people's livelihood and promoting the healthy development of society economy. In recent years, Changqing Oilfield actively implements national instructive spirit on vigorously promoting domestic oil and gas exploration and development strength and formulates the secondary acceleration development strategies. Its annual natural gas production in 2020 reaches 448.5 × 108 m3 and oil and gas equivalent exceeds 6000 × 104 t, tamping its important position of “Western Daqing” in this industry. In order to further promote the continuous stable production and realize the goal of quality and efficiency improvement in Changqing gas province, this paper analyzes the situations and challenges of natural gas development in this gas province, prepares the development technological countermeasures and forecast the natural gas development prospect. And the following research results are obtained. First, the natural gas exploration and development in Changqing gas province is divided into four stages and the main technologies for the development of low-permeability carbonate gas reservoir, low-permeability sandstone gas reservoir and tight sandstone gas reservoir are formed. Second, the natural gas development in Changqing gas province faces six challenges, such as low reserve production degree, sharp decline of production rates and complex distribution of remaining undeveloped reserves of developed gas fields. And twenty-six development technological countermeasures are put forward correspondingly, such as well pattern thickening, extension and reserve increase, secondary production layer potential tapping and fine gas well management. Third, the development direction and goal of natural gas development in Changqing gas province is to pay equal attention to the stable production of old gas fields and the production increase of new areas and carry out conventional gas and unconventional gas research simultaneously. In conclusion, the annual natural gas production of Changqing gas province will maintain a sound momentum of steady growth and will exceed 500 × 108 m3 at the end of the 14th Five-Year Plan and maintain at a stable level for a long term, which is conducive to the optimization of domestic energy consumption structure and realization of “carbon peak and carbon neutrality”

A Rice NBS-ARC Gene Conferring Quantitative Resistance to Bacterial Blight Is Regulated by a Pathogen Effector-Inducible miRNA

The bacterium Xanthomonas oryzae pv. Otyzae (Xoo) causes blight in rice worldwide, resulting in significant crop loss. However, no gene underlying a quantitative trait locus (QTL) for resistance against Xoo has been cloned yet. Here, we report the map-based cloning of a QTL, in which the NBS8R gene confers quantitative resistance to Xoo. NBS8R encodes an NB-ARC protein, which is involved in pathogen/microbe-associated molecular pattern-triggered immunity and whose expression is regulated by non-TAL effector XopQ-inducible Osa-miR1876 through DNA methylation. Sequence analysis of NBS8R in wild rice species and rice cultivars suggests that the Osa-miR1876 binding sites in the 5' UTR of NBS8R are inserted by chance and have undergone variations with Osa-miR1876 throughout evolution. The interaction between NBS8R and XopQ-inducible Osa-miR1876 is partially in keeping with the zigzag model, revealing that quantitative genes may also follow this model to control the innate immune response or basal disease resistance, and may prove valuable in utilizing the existing landraces that harbor the NBS8R gene but with no Osa-miR1876 binding site in rice breeding for bacterial blight resistance

DataSheet_1_Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice.zip

Papain-like cysteine proteases (PLCPs) play an important role in the immune response of plants. In Arabidopsis, several homologous genes are known to be involved in defending against pathogens. However, the effects of PLCPs on diseases that afflict rice are largely unknown. In this study, we show that a PLCP, an oryzain alpha chain precursor (OCP), the ortholog of the Arabidopsis protease RD21 (responsive to dehydration 21), participates in regulating resistance to blast disease with a shorter lesion length characterizing the knockout lines (ocp-ko), generated via CRISPR/Cas9 technology. OCP was expressed in all rice tissues and mainly located in the cytoplasm. We prove that OCP, featuring cysteine protease activity, interacts with OsRACK1A (receptor for activated C kinase 1) and OsSNAP32 (synaptosome-associated protein of 32 kD) physically in vitro and in vivo, and they co-locate in the rice cytoplasm but cannot form a ternary complex. Many genes related to plant immunity were enriched in the ocp-ko1 line whose expression levels changed significantly. The expression of jasmonic acid (JA) and ethylene (ET) biosynthesis and regulatory genes were up-regulated, while that of auxin efflux transporters was down-regulated in ocp-ko1. Therefore, OCP negatively regulates blast resistance in rice by interacting with OsRACK1A or OsSNAP32 and influencing the expression profiles of many resistance-related genes. Moreover, OCP might be the cornerstone of blast resistance by suppressing the activation of JA and ET signaling pathways as well as promoting auxin signaling pathways. Our research provides a comprehensive resource of PLCPs for rice plants in defense against pathogens that is also of potential breeding value.</p

DataSheet_2_Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice.docx

Papain-like cysteine proteases (PLCPs) play an important role in the immune response of plants. In Arabidopsis, several homologous genes are known to be involved in defending against pathogens. However, the effects of PLCPs on diseases that afflict rice are largely unknown. In this study, we show that a PLCP, an oryzain alpha chain precursor (OCP), the ortholog of the Arabidopsis protease RD21 (responsive to dehydration 21), participates in regulating resistance to blast disease with a shorter lesion length characterizing the knockout lines (ocp-ko), generated via CRISPR/Cas9 technology. OCP was expressed in all rice tissues and mainly located in the cytoplasm. We prove that OCP, featuring cysteine protease activity, interacts with OsRACK1A (receptor for activated C kinase 1) and OsSNAP32 (synaptosome-associated protein of 32 kD) physically in vitro and in vivo, and they co-locate in the rice cytoplasm but cannot form a ternary complex. Many genes related to plant immunity were enriched in the ocp-ko1 line whose expression levels changed significantly. The expression of jasmonic acid (JA) and ethylene (ET) biosynthesis and regulatory genes were up-regulated, while that of auxin efflux transporters was down-regulated in ocp-ko1. Therefore, OCP negatively regulates blast resistance in rice by interacting with OsRACK1A or OsSNAP32 and influencing the expression profiles of many resistance-related genes. Moreover, OCP might be the cornerstone of blast resistance by suppressing the activation of JA and ET signaling pathways as well as promoting auxin signaling pathways. Our research provides a comprehensive resource of PLCPs for rice plants in defense against pathogens that is also of potential breeding value.</p

DataSheet_3_Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice.xlsx

Papain-like cysteine proteases (PLCPs) play an important role in the immune response of plants. In Arabidopsis, several homologous genes are known to be involved in defending against pathogens. However, the effects of PLCPs on diseases that afflict rice are largely unknown. In this study, we show that a PLCP, an oryzain alpha chain precursor (OCP), the ortholog of the Arabidopsis protease RD21 (responsive to dehydration 21), participates in regulating resistance to blast disease with a shorter lesion length characterizing the knockout lines (ocp-ko), generated via CRISPR/Cas9 technology. OCP was expressed in all rice tissues and mainly located in the cytoplasm. We prove that OCP, featuring cysteine protease activity, interacts with OsRACK1A (receptor for activated C kinase 1) and OsSNAP32 (synaptosome-associated protein of 32 kD) physically in vitro and in vivo, and they co-locate in the rice cytoplasm but cannot form a ternary complex. Many genes related to plant immunity were enriched in the ocp-ko1 line whose expression levels changed significantly. The expression of jasmonic acid (JA) and ethylene (ET) biosynthesis and regulatory genes were up-regulated, while that of auxin efflux transporters was down-regulated in ocp-ko1. Therefore, OCP negatively regulates blast resistance in rice by interacting with OsRACK1A or OsSNAP32 and influencing the expression profiles of many resistance-related genes. Moreover, OCP might be the cornerstone of blast resistance by suppressing the activation of JA and ET signaling pathways as well as promoting auxin signaling pathways. Our research provides a comprehensive resource of PLCPs for rice plants in defense against pathogens that is also of potential breeding value.</p

Excavation of <i>Pid3</i> Orthologs with Differential Resistance Spectra to <i>Magnaporthe oryzae</i> in Rice Resource

<div><p>Twenty-six orthologs of the rice blast resistance gene <i>Pid3</i> from cultivated varieties and wild rice accessions distributed in different areas were cloned by allele mining. Sequence analysis showed that while each of the orthologous genes from <i>indica</i> varieties and most wild accessions encodes a complete NBS-LRR protein, each of the proteins encoded by those from <i>japonica</i> varieties and few wild rice accessions presents a premature termination. Eleven of the 26 orthologs were selected for blast resistance testing by transforming into the blast susceptible rice variety TP309, respectively. Inoculation of 23 <i>M. oryzae</i> strains collected from diverse regions of China to the respective transgenic plants revealed that 6 <i>Pid3</i> orthologs showed susceptible to all the tested strains, while the other 5 orthologs showed differential resistance spectra in a gradually spectrum-widen order as <i>Pid3-W3</i>, <i>Pid3-W4</i>, <i>Pid3-I3</i>, <i>Pid3-W5</i> and <i>Pid3-I1</i>. Amino acid sequences alignment of these orthologs indicated that the sequence diversities between the blast resistance orthologs were mostly located in the LRR domain such as the substitutions of Q694H,D856H,Q896R,D899E etc. However, the differences between the resistance orthologs and the susceptible ones were mostly located in the NBS domain. The present experiments provide an example of that the ortholog evaluation of plant <i>R</i> genes could be an efficient way to expand the rice blast resistance and some other plant disease resistance as well for breeding.</p></div

Detection of the DNA and RNA to the transformants.

<p>(A) HYG marker was used in the DNA detection. In addition, CAPS marker was used to confirm the positive transformants precisely and the restriction enzyme cutting site (<i>Bam</i>HI) lied in the premature stopped position of <i>Pid3-J1</i>; (B) Pid3D marker was used in the RNA detection, and Actin is as the referring primer marker.</p

Quantitative reverse-transcription polymerase chain reaction analysis of the transcript levels of <i>Pid3</i> orthologs.

<p>Relative quantity charted the expression levels of <i>Pid3</i> orthologs in TP309 (J1). TP309 (J1) was used as the control.</p