7 research outputs found
Amplification curves (A) and melting curves (B) of partial experimental strains.
<p>Amplification curves (A) and melting curves (B) of partial experimental strains.</p
Identification results of clinical pathogen strains.
<p>Identification results of clinical pathogen strains.</p
Ethidium-bromide–stained agarose gel of PCR reaction products from 12specimens through conventional method.
<p>Lane M, 100-base-pair (bp) DNA ladder; lane 1, reference strain of <i>Escherichia coli</i>; lane2, reference strain of <i>Pseudomonas aeruginosa</i>; lane 3, reference strain of <i>Staphylococcus aureus</i>; lane 4 to lane 12, representative clinical strain of <i>Escherichia coli 1-3</i>, <i>Staphylococcus aureus 1-3</i> and <i>Pseudomonas aeruginosa 1-3</i>; lane 13, negative control without DNA template.</p
Figure 1
<p>A: Three sizes of diameter punch (0.5-mm, 1-mm, 2-mm) involving higher and lower bacteria concentrations for PCR. −6 stands for 60 CFU ml<sup>−1</sup>, −1 stands for 6×10<sup>6</sup> CFU ml<sup>−1</sup>; B: Standard curve of real-time quantitative PCR of 6 decimal dilutions of representative Staphylococcus aureus ranging from 6×10<sup>4</sup> to 6×10<sup>9</sup> CFU ml<sup>−1</sup>.</p
Sequencing results of the 12 specimens separately performed by two methods.
<p>Sequencing results of the 12 specimens separately performed by two methods.</p
Amplification curves (A) and melting curves (B) of 12 specimens and negative control from improved method.
<p>Amplification curves (A) and melting curves (B) of 12 specimens and negative control from improved method.</p
Sequence quality from the 12 samples in two methods.
<p><sup>*</sup>using Wilcoxon Matched-Pairs Signed-Ranks Test.</p>#<p>using Matched-Pairs t-text.</p