The mode of tACE inhibition by QS.

<p>A. Lineweaver–Burk plot of the ACE activity in the presence of the hexapeptide; control (•), 100 µg/mL of QS (▴), and 200 µg/mL of QS (▪). B. The docking simulation of QS (green) binding to ACE (shown as sticks), and the overlap with captopril (cyan) in the crystal structure of the captopril-ACE complex The zinc ion (gray) is shown as nb_spheres. The figures were prepared using PYMOL software.</p

The docking simulation of TPTQQS binding to ACE.

<p>A. The docking simulation of TPTQQS (green) binding to ACE (shown as a multi-colored cartoon). A zinc ion (gray) was present in the active site of tACE. B. The interaction between TPTQQS (shown as sticks) and the residues of tACE (shown as lines) is shown.</p

The ITC titration curve.

<p>A. Binding of HHL to ACE at pH 8.3. B. Binding of HHL to ACE and TPTQQS at pH 8.3.</p

Understanding the Role of Few-Layer Graphene Nanosheets in Enhancing the Hydrogen Sorption Kinetics of Magnesium Hydride

The catalytic effects of few-layer, highly wrinkled graphene nanosheet (GNS) addition on the dehydrogenation/rehydrogenation performance of MgH₂ were investigated. It was found that MgH₂–5 wt %GNSs nanocomposites prepared by ball milling exhibit relatively lower sorption temperature, faster sorption kinetics, and more stable cycling performance than that of pure-milled MgH₂. The dehydrogenation step confirms that the Avrami exponent <i>n</i> increases from 1.22 to 2.20 by the Johnson–Mehl–Avrami (JMA) formalism when the desorption temperature is reduced from 350 °C to 320 °C and 300 °C, implying that a change in the decomposition temperature can alter the mechanism during the dehydrogenation process. For rehydrogenation, the Avrami value <i>n</i> is close to 1; further study by several models coincident with <i>n</i> = 1 reveals that the absorption process of the MgH₂–5 wt %GNSs sample conforms to the Mampel equation formulated through the random nucleation approach and that the nature of the absorption mechanism does not change within the temperature range studied. Furthermore, microstructure analysis demonstrated that the defective GNSs are distributed uniformly among the MgH₂ particles and that the grain size of the MgH₂–5 wt %GNSs nanocomposite is approximately 5–9 nm. The efficient metal-free catalytic dehydrogenation/rehydrogenation of MgH₂ can be attributed to the coupling of the nanosize effect and defective GNSs

Overlap of captopril (green) in the crystal structure of the captopril-ACE complex with TPTQQS (cyan) in the docking simulation for TPTQQS.

<p>The figures were prepared using PYMOL software.</p

Model of the inhibition of ACE by TPTQQS.

<p>The model shows that TPTQQS moves the zinc ion away from the active site to inhibit ACE.</p

Lineweaver–Burk plot of ACE activity in the presence of the hexapeptide.

<p>Control (•), 100 µg/mL of the hexapeptide (▴), and 200 µg/mL of the hexapeptide (▪).</p

The inhibitory activity of the modified peptides from TPTQQS.

<p>The concentration of each peptide was 0.25 mmol/L.</p

Analysis of <i>mur33</i> promoter by catechol dioxygenase activity assay.

<p>(A) The enzyme activities for the seed cultures of WT/pJTU5034, WT/pJTU5037 and WT/pJTU5038. (B) The enzyme activities for the seed cultures of DM-5/pJTU5034, DM-5/pJTU5037 and DM-5/pJTU5038. (C) The enzyme activities for the seed cultures of WT/pJTU5034 and DM-5/pJTU5034. (D) The enzyme activities for the fermentation cultures of WT/pJTU5034 and DM-5/pJTU5034. All histograms showed the quantitative catechol dioxygenase activity of <i>Streptomyces</i> sp. NRRL30471 and DM-5 independently containing pJTU5034, pJTU5037, pJTU5038 and pJTU3700. WT/pJTU3700 indicates <i>Streptomyces</i> sp. NRRL 30471 containing pJTU3700 (no <i>mur33</i> promoter) is as the negative control. WT/pJTU5034, indicates <i>Streptomyces</i> sp. NRRL 30471 containing pJTU5034 (natural <i>mur33</i> promoter). WT/pJTU5037 indicates <i>Streptomyces</i> sp. NRRL 30471 containing pJTU5037 (the -10 region mutated on <i>mur33</i> promoter). WT/pJTU5038 indicates <i>Streptomyces</i> sp. NRRL 30471 containing pJTU5038 (the -35 region mutated on <i>mur33</i> promoter). Likewise, DM-5 derived strains were designated.</p

Ultrasmall NiFe-Phosphate Nanoparticles Incorporated α‑Fe₂O₃ Nanoarrays Photoanode Realizing High Efficient Solar Water Splitting

The practical application of hematite (α-Fe₂O₃) in solar water splitting is severely limited by the highly charge recombination rate though its abundant reserves and suitable bandgap of ∼2.1 eV. This work describes the synthesis of ultrasmall NiFe-phosphate (NFP) nanoparticles incorporated α-Fe₂O₃ nanoarrays photoanode via a facile dip-coating and annealing process to demonstrate combined effects on enhanced photoelectrochemical (PEC) water oxidation. The NFP uniformly decorating on the surface of hematite nanorods not only could improve water oxidation kinetics and charge separation efficiency, but also could suppress the charge recombination in company with the surface states passivation. Furthermore, the phosphate (P) in the NFP nanoparticles could also play a synergistic effect on promoting the multiproton-coupled electron transfer (PCET) process for the PEC water oxidation. All of these lead to ∼140 mV cathodic shift of onset potential, ∼2.3-fold enhancement of the photocurrent and excellent long-term stability at 1.23 V_RHE in 0.1 M KOH solution for α-Fe₂O₃/NFP photoanode. Along with these advantages, the NFP nanoparticles may possess new opportunities for modulating PEC water oxidation performances in hematite and other metal oxide photoanodes