3 research outputs found

    Morphology of reconstituted mammary glands of both strains into a background

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    <p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Mammary cells were isolated from 2-month-old BALB/c or C57BL/6 mice and 5 μl of cell suspensions were transplanted into the right or left cleared mammary fat pads, respectively, of 21-day-old Swiss or syngeneic mice. At surgery pellets containing Eplus Pg or plus MPA were also implanted subcutneously into the backs of the animals. One month later, animals were killed and mammary glands excised and fixed. Microscopic examination revealed no changes between C57BL/6 or BALB/c repopulated Swiss /glands, whereas C57BL/6 cells transplanted in syngeneic glands were under-developed (100×, bar: 700 μm). A representative image of the WM or the H&E stained slides of the reconstituted mammary glands in E/Pg-treated mice is shown. Microsatellite studies confirmed the strain origin of the epithelial cells in the Swiss background. bp, base pairs; E, 17β-estradiol; H&E, hematoxylin and eosin; LCM, laser capture microdissection; MPA, medroxyprogesterone acetate; Pg, progesterone; WM, whole mount

    PR expression in mammary glands treated with progestins for 2 months: Western blot studies

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    <p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Mammary glands from the same groups shown in Figure 4 were processed to obtain nuclear extracts (as explained in Materials and methods) and used in Western blots. A representative experiment using C-19 PR Santa Cruz antibody is shown. Because different amounts of epithelial cells are present in the different extracts, the ratio of PR-A/PR-B in three different experiments is shown. *< 0.05, **< 0.01, ***< 0.001 treated versus control. PR, progesterone receptor

    Effects of progestins in salivary gland morphology and in serum pituitary hormone levels

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    <p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Salivary glands from control, MPA, or Pg-treated BALB/c or C57BL/6 mice. Pellets were implanted subcutaneously and the animals killed after 2 months. MPA increased the development of convoluted granular ducts (arrow). No differences were observed between both strains of mice (400×, bar: 120 μm). Serum PRL, Pg, GH, and IGF-I levels measured by radioimmunoassay in control BALB/c or C57BL/6 female mice and after 24 hours of MPA or Pg treatment. No differences were detected in C57BL/6 mice as compared with BALB/c mice. Furthermore, Pg and MPA treatment induced an increase in PRL levels in both strains (< 0.001, two-way ANOVA). The increase in Pg levels after progesterone administration was also similar in both strains (< 0.001, two-way ANOVA). Gray bars: BALB/c; black bars: C57BL/6. ANOVA, analysis of variance; GH, growth hormone; IGF, insulin-like growth factor; MPA, medroxyprogesterone acetate; Pg, progesterone; PR, progesterone receptor; PRL, prolactin
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