Association of polymorphisms in the IGF1, GH and PIT1 genes with growth and reproductive traits in Canchim cattle

A variance component approach for quantitative trait loci (QTL) mapping was proposed by George et al. (2000), which assumes a mixed inheritance model (polygenes and QTL effects). This approach allows for mapping QTL in general pedigrees from outbreed populations. In this method, besides the use of an additive relationship matrix to estimate the additive polygenic effects, an identical-by-descent (IBD) matrix is used to estimate the QTL effect and its variance. Analyses of the association of genetic markers with economic traits in beef cattle have been mainly reported in developed countries. In the Brazilian Canchim breed, studies found association of polymorphism in the insulin-like growth factor 1 (IGF1), growth hormone (GH) and specific pituitary transcriptions (PIT1) genes with growth traits (Pereira et al. (2005), Andrade et al. (2008) and Carrijo et al. (2008)). However, no studies were found that used the variance component approach to estimate the genetic variance due to these genes, or QTLs linked to them, in Canchim cattle. The objective of this study was to estimate the proportion of phenotypic variance of growth and reproductive traits that are explained by polymorphisms in the IGF1, GH and PIT1 genes in Canchim cattle.1 CD-RO

Association of apolipoprotein B and adiponectin receptor 1 genes with carcass, bone integrity and performance traits in a paternal broiler Line.

Apolipoprotein B (APOB) and Adiponectin Receptor 1 (ADIPOR1) are related to the regulation of feed intake, fat metabolism and protein deposition and are candidate genes for genomic studies in birds. In this study, associations of two single nucleotide polymorphisms (SNPs) g.102A>T (APOB) and g.729C>T (ADIPOR1) with carcass, bone integrity and performance traits in broilers were investigated. Genotyping was performed on a paternal line of 1,454 broilers. The SNP detection was carried out by PCR-RFLP technique using the restriction enzymes HhaI for the SNP g.729C>T and MslI for the SNP g.102A>T. The association analyses of the two SNPs with 85 traits were performed using the restricted maximum likelihood (REML) and Generalized Quasi-Likelihood Score (GQLS) methods. For REML the model included the random additive genetic effect of animal and fixed effects of sex, hatch and SNP genotypes. In the GQLS method, a logistic regression was used to associate the genotypes with phenotypes adjusted for fixed effects of sex and hatch. The SNP g.729C>T in the ADIPOR1 gene was associated with thickness of the femur and breast skin yield. Thus, the ADIPOR1 gene seems implicated in the metabolism and/or fat deposition and bone integrity in broilers

Tissue tropism in host transcriptional response to members of the bovine respiratory disease complex.

Bovine respiratory disease (BRD) is the most common infectious disease of beef and dairy cattle and is characterized by a complex infectious etiology that includes a variety of viral and bacterial pathogens. We examined the global changes in mRNA abundance in healthy lung and lung lesions and in the lymphoid tissues bronchial lymph node, retropharyngeal lymph node, nasopharyngeal lymph node and pharyngeal tonsil collected at the peak of clinical disease from beef cattle experimentally challenged with either bovine respiratory syncytial virus, infectious bovine rhinotracheitis, bovine viral diarrhea virus, Mannheimia haemolytica or Mycoplasma bovis. We identified signatures of tissue-specific transcriptional responses indicative of tropism in the coordination of host?s immune tissue responses to infection by viral or bacterial infections. Furthermore, our study shows that this tissue tropism in host transcriptional response to BRD pathogens results in the activation of different networks of response genes. The differential crosstalk among genes expressed in lymphoid tissues was predicted to be orchestrated by specific immune genes that act as ?key players? within expression networks. The results of this study serve as a basis for the development of innovative therapeutic strategies and for the selection of cattle with enhanced resistance to BRD.Made available in DSpace on 2019-02-21T00:47:34Z (GMT). No. of bitstreams: 1 11TissueTropisminHostTranscriptionalResponsetoMembersoftheBovineRespiratoryDiseaseComplex.pdf: 2935433 bytes, checksum: 7f2db937dad9b124831b34007d4ab7a1 (MD5) Previous issue date: 2019-02-20bitstream/item/193126/1/11-Tissue-Tropism-in-Host-Transcriptional-Response-to-Members-of-the-Bovine-Respiratory-Disease-Complex.pd