Teil 4 : Wasser sparen im Ackerbau

Wasser ist ein begrenzender Produktionsfaktor für den Ackerbau im Süden der Metropolregion Hamburg. Im Hinblick auf den Klimawandel und eine begrenzte Wasserverfügbarkeit muss das vorhandene Wasser effizienter genutzt werden. In KLIMZUG-NORD wurden hierzu im Rahmen von Feldversuchen verschiedene Anpassungsmaßnahmen erprobt

Development and Single Laboratory Evaluation of a Refined and specific Real-time PCR Detection Method, Using Mitochondrial Primers (Mit1C), for the Detection of Cyclospora cayetanensis in Produce

Regulatory methods for detection of the foodborne protozoan parasite Cyclospora cayetanensis must be specific and sensitive. To that end, we designed and evaluated (in a single laboratory validation) a novel and improved primer/probe combination (Mit1C) for real-time PCR detection of C. cayetanensis in produce. The newly developed primer/probe combination targets a conserved region of the mitochondrial genome of C. cayetanensis that varies in other closely related organisms. The primer/probe combination was evaluated both in silico and using several real-time PCR kits and polymerases against an inclusivity/exclusivity panel comprised of a variety of C. cayetanensis oocysts, as well as DNA from other related Cyclospora spp. and closely related parasites. The new primer/probe combination amplified only C. cayetanensis, thus demonstrating specificity. Sensitivity was evaluated by artificially contaminating cilantro, raspberries, and romaine lettuce with variable numbers (200 and 5) of C. cayetanensis oocysts. As few as 5 oocysts were detected in 75%, 67.7%, and 50% of the spiked produce samples (cilantro, raspberries, and romaine lettuce), respectively, all uninoculated samples and no-template real-time PCR controls were negative. The improved primer/probe combination should prove an effective analytical tool for the specific detection of C. cayetanensis in produce

Solvothermal processes and the synthesis of nitrides

Nitrides constitute an important class of materials due to their physical properties. In this way, all processes related to their synthesis or shaping are of relevant interest. A solvothermal process can be described as a reaction between precursors in a close system in the presence of a solvent and at a temperature higher than the boiling point of the solvent, with pressure being consequently involved. Depending on the pressure and temperature, the solvent is in sub- or supercritical conditions. The system precursors/solvent can be either homogeneous or heterogeneous. Concerning nitrides, solvothermal processes have been mainly developed in three domains: 1. the preparation of novel compositions / 2. the elaboration of nanocrystallites well defined in size and morphology / 3. the crystal-growth of functional nitrides such as GaN. Considering the synthesis of novel nitrides, different solvothermal reactions can be used versus the nitriding agent (N3- or N./GHPR_A_350185_O_XML_IMAGES/GHPR_A_350185_O_ILM0001.gif ) and the nature of the solvent (nitriding solvent or non-reactive solvent). Different examples will be given and the potentialities of solvothermal processes in the preparation of novel nitrides will be discussed