10 research outputs found
H+/2e- stoichiometry of the NADH : ubiquinone reductase reaction catalyzed by submitochondrial particles
Synthesis and Herbicidal and Antioxidant Activity of a Series of Hetero- and Carbocyclic Derivatives of Monochloroacetic Acid
Generation of superoxide-radical by the NADH: Ubiquinone oxidoreductase of heart mitochondria
Oscillations of the redox state of the nicotinamide adenine dinucleotide coenzime in an aqueous medium and in microemulsions upon interaction with an endogenous source of nitrogen oxide: S-nitrosoglutathione
Kinetic Model of Mitochondrial Krebs Cycle: Unraveling the Mechanism of Salicylate Hepatotoxic Effects
This paper studies the effect of salicylate on the energy metabolism of mitochondria using in silico simulations. A kinetic model of the mitochondrial Krebs cycle is constructed using information on the individual enzymes. Model parameters for the rate equations are estimated using in vitro experimental data from the literature. Enzyme concentrations are determined from data on respiration in mitochondrial suspensions containing glutamate and malate. It is shown that inhibition in succinate dehydrogenase and α-ketoglutarate dehydrogenase by salicylate contributes substantially to the cumulative inhibition of the Krebs cycle by salicylates. Uncoupling of oxidative phosphorylation has little effect and coenzyme A consumption in salicylates transformation processes has an insignificant effect on the rate of substrate oxidation in the Krebs cycle. It is found that the salicylate-inhibited Krebs cycle flux can be increased by flux redirection through addition of external glutamate and malate, and depletion in external α-ketoglutarate and glycine concentrations