An Updated Checklist of the Sicilian Native Edible Plants: Preserving the Traditional Ecological Knowledge of Century-Old Agro-Pastoral Landscapes

The traditional use of native wild food plants (NWFP) may represent a valuable supplementary food source for the present and future generations. In Sicily, the use of wild plants in the human diet dates back to very ancient times and still plays an important role in some rural communities. Moreover, in this regard, the natural and cultural inheritance of this island is wealthy and diversified for several reasons. First, Sicily hosts a rich vascular flora, with 3,000 native and 350 endemic plants. Second, due to its central position in the Mediterranean, the island has acted as a veritable melting pot for the ethnobotanical knowledge of the rural communities of the entire basin. We reviewed all the available literature and, starting from such omnicomprehensive checklist, partially improved thanks to the data issuing from recent field investigations, we critically revised the whole species list, basing our review on field data issuing from interviews and on our expert knowledge. As a result, we provide a substantially updated list of 292 NWFP growing on the island. Further 34 species, reported as NWFP on previous papers were discarded because they are not native to Sicily, while 45 species were listed separately because their identity, occurrence and local use as food is doubtful and needs to be further investigated. Moreover, we tried to shed light on the ecology (growth form and preferential habitat) of the Sicilian NWFP, with special focus on crop wild relatives (CWR). Our preliminary ecological analyses point out that a high percentage of these plants are linked with the so-called ‘cultural’ landscapes, patchy semi-natural environments rich in ecotones, leading to the conclusion that the maintenance of century-old agro-pastoral practices may represent an effective way to preserve the local heritage of edible plants. Our study allowed to identify as much as 102 taxa of agronomic interest which could be tested as novel crops in order to face ongoing global changes and to comply with sustainable agriculture policies. Among them, 39 taxa show promising traits in terms of tolerance to one or more environmental stress factors, while 55 more are considered CWR and/or can be easily cultivated and/or show high productivity/yield potential

Molecular Analysis of the Official Algerian Olive Collection Highlighted a Hotspot of Biodiversity in the Central Mediterranean Basin

Genetic diversity and population structure studies of local olive germplasm are important to safeguard biodiversity, for genetic resources management and to improve the knowledge on the distribution and evolution patterns of this species. In the present study Algerian olive germplasm was characterized using 16 nuclear (nuSSR) and six chloroplast (cpSSR) microsatellites. Algerian varieties, collected from the National Olive Germplasm Repository (ITAFV), 10 of which had never been genotyped before, were analyzed. Our results highlighted the presence of an exclusive genetic core represented by 13 cultivars located in a mountainous area in the North-East of Algeria, named Little Kabylie. Comparison with published datasets, representative of the Mediterranean genetic background, revealed that the most Algerian varieties showed affinity with Central and Eastern Mediterranean cultivars. Interestingly, cpSSR phylogenetic analysis supported results from nuSSRs, highlighting similarities between Algerian germplasm and wild olives from Greece, Italy, Spain and Morocco. This study sheds light on the genetic relationship of Algerian and Mediterranean olive germplasm suggesting possible events of secondary domestication and/or crossing and hybridization across the Mediterranean area. Our findings revealed a distinctive genetic background for cultivars from Little Kabylie and support the increasing awareness that North Africa represents a hotspot of diversity for crop varieties and crop wild relative species

From glacial refugia to hydrological microrefugia: Factors and processes driving the persistence of the climate relict tree Zelkova sicula

With only two tiny populations, the climate relict Zelkova sicula (Sicily, Italy) is one of the rarest trees in the world. It also represents the most marginal member of genus Zelkova that was widespread in the broadleaved forests thriving in warm-temperate climates throughout Eurasia until the Last Glacial Age. Occurring at the westernmost range of the genus under typical Mediterranean climate, the micro-topographic settings have always appeared crucial for the survival of this relict. However, the factors and processes actually involved in its persistence in the current refugia, as well as the response of similar relict trees in arid environments, are poorly understood worldwide. In the aim to elucidate these aspects, in the two sites hosting Z. sicula analyses of topographical attributes were combined with investigations on soil moisture dynamics. Additionally, plants' growth and spatial distribution patterns were analyzed to detect fine-scale differences between populations and assess the possible ecological amplitude of the species. Results revealed that convergent topographies are basic determinants of microrefugia in arid environments. Within the investigated sites, underground moisture never decreases below 25%, buffering seasonal rainfall fluctuations. Therefore, hydrological microrefugia play a key role in decoupling from regional climate, supporting the target species in coping with an unsuitable climatic envelope. Additionally, the inter-population variability of biometric attributes showed that individual growth is site-dependent and the species retains a relative ecological plasticity, whereas the strongly clumped spatial patterns confirmed the common clonal growth. On one hand, deeply incised landforms have acted as effective hydrologic microrefugia, on the other clonality coupled with triploidy supposedly improved the resistance of Z. sicula to harsh environments, though entailing inability to reproduce sexually. Most likely, sterility and environmental/physical barriers that have existed for millennia have prevented this relict from leaving the last suitable microrefugia, resulting in the two current rear edge populations

Conservation of Ptilostemon greuteri (Asteraceae), an endemic climate relict from Sicily (Italy): state of knowledge after the discovery of a second population

Ptilostemon greuteri is one of the most endangered and poorly studied woody vascular plant species of the Mediterranean Basin, endemic to Sicily (Italy). Several peculiar traits confer to P. greuteri a character of uniqueness and an enigmatic attractiveness. In fact, at first glimpse the largest individuals of this species remind the most remarkable and peculiar cases of herbaceous genera (e.g., Echium, Senecio) turned woody on insular or insular-like mountain ecosystems. Following the discovery of a second population of this rare species, a project aiming at the study and conservation of P. greuteri was set up. We present here updated information on the distribution, conservation status, biology and ecological requirements of this species. Field surveys supported by drone imagery were carried out to estimate the current range of the two populations. The data obtained allowed to refine the conservation status assessment of the species and to update the distribution pattern of P. greuteri by means of detailed digital maps of each population. Ongoing field investigations currently provide valuable information about the ecological requirements of the species and point out the impact of wildfires on population size and structure. Our research has also highlighted several knowledge gaps that need to be filled to apply more effective conservation measures. Preliminary results suggest that local topographic and mesoclimatic factors (air humidity and shade) are key variables explaining the persistency and survival of P. greuteri in the area

Plant hunting: exploring the behaviour of amateur botanists in the field

We asked what are the behavioural and logistic preferences of professional and amateur botanists when exploring flora in the field. We extracted temporal and spatial data on vas- cular plant species occurrences from three datasets of Sicilian flora: a subset of iNaturalist, a dataset collected by a Facebook group focused on the flora of Sicily and a subset of the professional database European Vegetation Archive. We used the time span of individual contributor’s activity as a proxy of their commitment to collecting information about the flora of Sicily. Climate and landscape data were used to better characterize the spatial and temporal activity of data contributors. Finally, we assessed which habitat types were more frequently visited in each dataset. Our results suggest that amateur botanists can be divided into two categories, "experienced" and "occasional", playing different roles on digital plat- forms. While experienced amateur botanists are characterized by prolonged activity and many posts, occasional ones have short and erratic activity but collectively compile more data than the former category. The occasional botanists represent a new phenomenon in data collection, supported by the spread of digital platforms. While in the past, experienced botanists were primarily involved in biodiversity data collection, at present, they also play a fundamental role in validating the observations by occasional botanists and disseminat- ing floristic knowledge to them. Our study points out that the interaction through social media can catalyze the establishment of participatory monitoring networks, promote con- servation-oriented initiatives and influence policy-makers

Phylogenetic Relationship Among Wild and Cultivated Grapevine in Sicily: A Hotspot in the Middle of the Mediterranean Basin

Grapevine (Vitis vinifera ssp. sativa) is a perennial crop especially important for wine and fruit production. The species is highly polymorphic with thousands of different varieties selected by farmers and clonally propagated. However, it is still debated whether grapevine domestication from its wild ancestor (V. vinifera ssp. sylvestris) has been a single event or rather it occurred on multiple occasions during the diffusion of its cultivation across the Mediterranean. Located in the center of the Basin, Sicily is its largest island and has served as a hotspot for all civilizations that have crossed the Mediterranean throughout history. Hundreds of unique grapevine cultivars are still cultivated in Sicily and its surrounding minor islands, though most of them are menaced by extinction. Wild grapevine is also present with isolated populations thriving along riverbanks. With the aim to evaluate the phylogenetic relationships among Sicilian varieties, and to assess the possible contribution of indigenous wild populations to the genetic makeup of cultivated grapevine, we analyzed 170 domestic cultivars and 125 wild plants, collected from 10 different populations, with 23 SSR markers. We also compared our data with published dataset from Eurasia. Results show that Sicilian wild populations are related to the cultivated Sicilian and Italian germplasm, suggesting events of introgression and/or domestication of local varieties

A multifaceted field sampling approach for the management of extremely narrow endemic vascular plant species

Extremely narrow endemic plant species (ENEs) are generally connected with micro- refugia characterized by particular environmental conditions. In-depth knowledge of the ecological requirements of ENEs is fundamental to plan appropriate conservation measures. Using cross-cutting technology, this paper gives a multifaceted approach to collect on-site data on the ecology of ENEs, defines the protocols for a correct sampling design and describes the type of equipment, the time and expenditure needed. Our sampling approach is based on two orthogonal transects, long enough to extrapolate the whole ecological gradient across the area of occupancy of the target species. Microclimatic data are recorded all along the transects through iBut- ton technology, plus a weather station installed at the intersection of the transects. Microtopographic data are recorded with high-resolution digital elevation model and sub-metric GPS. Edaphic data are recorded along the transects through standard soil analyses and on-site evaluation of the seasonal decomposition rate of organic matter. Additionally, vegetation sampling in 4 m2 plots and on-site germination tests allow to collect data on auto- and synecological factors that regulate the life cycle of the target species. Our approach has proved to be cost-effective and efficient in terms of time spent in the field against the data collected. The most demanding activities were the establishment of the transects and the vegetation sampling. The time spent downloading microclimatic data and testing seed germination was relatively short. Our sampling design allows: (i) to catch as much micro-topographic variability as pos- sible, both within and out of the tolerance range of the target species, (ii) to minimize the risk of recording identical micro-topographic conditions compared with a random sampling scheme, and (iii) to ensure quick and relatively easy retrieval of the plots and the equipment both on a multi-seasonal and multi-annual basis

Facebook groups as citizen science tools for plant species monitoring

I social network sono canali di comunicazione utilizzati per condividere enormi quantità di dati, che possono essere utilizzati per la ricerca scientifica, anche nel campo della biodiversità. Per sapere quanto i dati ricavati dai social network possono integrare quelli raccolti per scopi scientifici, è necessario individuarne i bias. Utilizzando i dati estratti da un gruppo Facebook specializzato nella flora vascolare siciliana, abbiamo analizzato quali sono i caratteri che aumentano la probabilità che una pianta spontanea venga fotografata e postata su un social network. A tal fine, abbiamo confrontato frequenze e attributi delle specie fotografate dai membri del gruppo Facebook con quelli delle specie registrate nel database EVA, relative a 6.366 rilevamenti vegetazionali della Sicilia. I nostri risultati suggeriscono che i dati provenienti dal gruppo Facebook analizzato sono affetti da bias diversi da quelli relativi a dati floristici raccolti per altri fini, ad esempio, per il rilevamento della vegetazione. I membri del gruppo Facebook hanno intercettato con maggior frequenza specie aliene e specie rare rispetto a quanto rilevato da chi studia la vegetazione, dimostrando che i dati contenuti nei social network offrono un importante complemento a quelli raccolti dagli scienziati per fini di ricerca. Sintesi e applicazioni. Malgrado Facebook non supporti la georeferenziazione dei dati, né una query per l'accesso e l'analisi degli stessi, esso rappresenta una potenziale fonte di dati sulla biodiversità, utili per integrare quelli raccolti da botanici professionisti. Il principale vantaggio dei dati provenienti dai social network è il loro elevato dinamismo, poiché registrano la presenza di una data specie quasi in tempo reale. Pertanto, i dati raccolti da gruppi Facebook moderati da esperti possono risultare utili (1) per monitorare le dinamiche di popolazione di specie minacciate ed esotiche; (2) come informazioni aggiuntive sulla presenza di specie rare, in particolare per quelle che maggiormente attraggono i botanici dilettanti, come le orchidee; (3) per individuare tempestivamente la presenza di nuove esotiche potenzialmente invasive; (4) per studi fenologici, in particolare per monitorare l’inizio della stagione di fioritura delle specie.Social networks offer communication channels through which people share huge amounts of primary data that can be used for scientific analyses, including biodiversity research. To understand to what extent data extracted from social networks could complement data collected for scientific purposes, it is necessary to quantify the bias of such data. We analysed which plant traits increased the probability of a wild‐growing plant species to be photographed and posted to a social network based on the data from an unstructured citizen science tool; a Facebook group focused on the vascular flora of Sicily (Italy). Then, we compared botanical data collected by this Facebook group members with data collected by scientists in 6,366 vegetation plots sampled across Sicily, stored in the EVA database. Our results suggested that data proceeding from the analysed Facebook group were affected by various sampling biases, which differed from the biases inherent to other types of biodiversity data such as those from vegetation plots. Facebook users recorded a higher proportion of red‐listed and alien species than vegetation scientists. Therefore, social networks can provide a valuable complement to the data collected by scientists for research purposes. Synthesis and applications. Despite Facebook does not support geotagging and interface for data access and analysis, it is an invaluable source of biodiversity data that could complement those collected by professional researchers. The main advantage of data from social networks is their high dynamism, as they report large amounts of species occurrences in almost real time. Therefore, citizen science data from a Facebook group where the records are curated by expert volunteers can be used (a) for monitoring population dynamics of threatened and alien species; (b) as a source of additional data on rare species occurrences, particularly for plants that are attractive for amateur botanists, such as orchids; (c) for early warning systems of potential new invasions; and (4) for phenological studies, especially at the beginning of the flowering season

Relevant habitats neglected by the Directive 92/43 EEC: the contribution of Vegetation Science for their reappraisal in Sicily

Field investigation carried out by the Sicilian botanists in the last 20 years enabled them to identify eight habitat types of high biogeographic and conservation interest, neglected by the Directive 92/43, which deserve ad hoc conservation measures. For each of these habitats, a syntaxonomic interpretation of the corresponding plant communities, their main ecological, physiognomic and syndynamic traits and a list of diagnostic species are provided. Their classification into the macrotypes listed in the Annex I of the Directive 92/43 and the respective correspondence in EUNIS habitat classification are proposed. The habitats here described integrate those already proposed by the Italian Botanical Society, with the hope of an adequate recognition at national at supranational level