47 research outputs found
Tubulin bond energies and microtubule biomechanics determined from nanoindentation in silico
Microtubules, the primary components of the chromosome segregation machinery,
are stabilized by longitudinal and lateral non-covalent bonds between the
tubulin subunits. However, the thermodynamics of these bonds and the
microtubule physico-chemical properties are poorly understood. Here, we explore
the biomechanics of microtubule polymers using multiscale computational
modeling and nanoindentations in silico of a contiguous microtubule fragment. A
close match between the simulated and experimental force-deformation spectra
enabled us to correlate the microtubule biomechanics with dynamic structural
transitions at the nanoscale. Our mechanical testing revealed that the
compressed MT behaves as a system of rigid elements interconnected through a
network of lateral and longitudinal elastic bonds. The initial regime of
continuous elastic deformation of the microtubule is followed by the transition
regime, during which the microtubule lattice undergoes discrete structural
changes, which include first the reversible dissociation of lateral bonds
followed by irreversible dissociation of the longitudinal bonds. We have
determined the free energies of dissociation of the lateral (6.9+/-0.4
kcal/mol) and longitudinal (14.9+/-1.5 kcal/mol) tubulin-tubulin bonds. These
values in conjunction with the large flexural rigidity of tubulin
protofilaments obtained (18,000-26,000 pN*nm^2), support the idea that the
disassembling microtubule is capable of generating a large mechanical force to
move chromosomes during cell division. Our computational modeling offers a
comprehensive quantitative platform to link molecular tubulin characteristics
with the physiological behavior of microtubules. The developed in silico
nanoindentation method provides a powerful tool for the exploration of
biomechanical properties of other cytoskeletal and multiprotein assemblie
Kinetochore-microtubule attachment throughout mitosis potentiated by the elongated stalk of the kinetochore kinesin CENP-E
Centromere protein E (CENP-E) is a highly elongated kinesin that transports pole-proximal chromosomes during congression in prometaphase. During metaphase, it facilitates kinetochore-microtubule end-on attachment required to achieve and maintain chromosome alignment. In vitro CENP-E can walk processively along microtubule tracks and follow both growing and shrinking microtubule plus ends. Neither the CENP-E-dependent transport along microtubules nor its tip-tracking activity requires the unusually long coiled-coil stalk of CENP-E. The biological role for the CENP-E stalk has now been identified through creation of "Bonsai" CENP-E with significantly shortened stalk but wild-type motor and tail domains. We demonstrate that Bonsai CENP-E fails to bind microtubules in vitro unless a cargo is contemporaneously bound via its C-terminal tail. In contrast, both full-length and truncated CENP-E that has no stalk and tail exhibit robust motility with and without cargo binding, highlighting the importance of CENP-E stalk for its activity. Correspondingly, kinetochore attachment to microtubule ends is shown to be disrupted in cells whose CENP-E has a shortened stalk, thereby producing chromosome misalignment in metaphase and lagging chromosomes during anaphase. Together these findings establish an unexpected role of CENP-E elongated stalk in ensuring stability of kinetochore-microtubule attachments during chromosome congression and segregation
Unique Role of Vimentin Networks in Compression Stiffening of Cells and Protection of Nuclei from Compressive Stress
In this work, we investigate whether stiffening in compression is a feature of single cells and whether the intracellular polymer networks that comprise the cytoskeleton (all of which stiffen with increasing shear strain) stiffen or soften when subjected to compressive strains. We find that individual cells, such as fibroblasts, stiffen at physiologically relevant compressive strains, but genetic ablation of vimentin diminishes this effect. Further, we show that unlike networks of purified F-actin or microtubules, which soften in compression, vimentin intermediate filament networks stiffen in both compression and extension, and we present a theoretical model to explain this response based on the flexibility of vimentin filaments and their surface charge, which resists volume changes of the network under compression. These results provide a new framework by which to understand the mechanical responses of cells and point to a central role of intermediate filaments in response to compression
Mechanisms to Avoid and Correct Erroneous Kinetochore-Microtubule Attachments
In dividing vertebrate cells multiple microtubules must connect to mitotic kinetochores in a highly stereotypical manner, with each sister kinetochore forming microtubule attachments to only one spindle pole. The exact sequence of events by which this goal is achieved varies considerably from cell to cell because of the variable locations of kinetochores and spindle poles, and randomness of initial microtubule attachments. These chance encounters with the kinetochores nonetheless ultimately lead to the desired outcome with high fidelity and in a limited time frame, providing one of the most startling examples of biological self-organization. This chapter discusses mechanisms that contribute to accurate chromosome segregation by helping dividing cells to avoid and resolve improper microtubule attachments