Interactions among multiple selective pressures on the form–function relationship in insular stream fishes

Relationships between body shape and escape performance are well established for many species. However, organisms can face multiple selection pressures that might impose competing demands. Many fishes use fast starts for escaping predator attacks, whereas some species of gobiid fishes have evolved the ability to climb waterfalls out of predator-dense habitats. The ancestral ‘powerburst’ climbing mechanism uses lateral body undulations to move up waterfalls, whereas a derived ‘inching’ mechanism uses rectilinear locomotion. We examined whether fast-start performance is impacted by selection imposed from the new functional demands of climbing. We predicted that non-climbing species would show morphology and fast-start performance that facilitate predator evasion, because these fish live consistently with predators and are not constrained by the demands of climbing. We also predicted that, by using lateral undulations, powerburst climbers would show escape performance superior to that of inchers. We compared fast starts and body shape across six goby species. As predicted, non-climbing fish exhibited distinct morphology and responded more frequently to an attack stimulus than climbing species. Contrary to our predictions, we found no differences in escape performance among climbing styles. These results indicate that selection for a competing pressure need not limit the ability of prey to escape predator attacks

A systematic survey evaluating 6-thioguanine-related hepatotoxicity in patients with inflammatory bowel disease

Objective: Drug-induced liver injury was recently reported as a major complication leading to hepatic nodular regenerative hyperplasia (NRH) in patients with inflammatory bowel disease (IBD) and 6-thioguanine (6-TG) therapy. The aim of the study was to evaluate the prevalence of 6-TG-related hepatotoxicity in a large multi-centered IBD population by means of a systematic online survey. Methods: Clinical and laboratory data, imaging techniques (sonography, CT, MRI) and histology of liver biopsies were surveyed in IBD patients treated with 6-TG. The decision on whether liver imaging and/or liver biopsy were performed was exclusively at the discretion of the investigator. Results: 6-TG use was fully documented in 296 patients (median treatment duration 56 weeks, range < 1–207). Laboratory signs of drug-induced liver injury were found in 43 patients (14.5%). Liver imaging revealed pathologic results in 68/176 patients (38.6%). Liver biopsy was performed in a subset of 60 patients; using silver-reticulin staining (n = 59), NRH was considered in 16 patients (27.1%). Age was the only independent, albeit weak, risk factor for development of NRH. Conclusion: This large online survey confirms the strong association between 6-TG treatment and the significant risk of development of NRH in patients with IBD. The definitive diagnosis of NRH depends solely upon liver biopsy