Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80°C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating affected skeletal muscles

Thermal regimes of HTS cylinders operating in devices for fault current limitation

We reveal obstacles related to the application of HTS cylinders in current limiting devices based on the superconducting - normal state transition. It is shown that, at the critical current density achieved presently in bulk materials, and especially in BSCCO-2212, the required thickness of the cylinder wall in a full-scale inductive device achieves several centimeters. A simple mathematical model of the operation of an inductive fault current limiter (FCL) is used to show that such cylinders cannot be cooled in admissible time after a fault clearing and, hence, the inductive FCLs and current-limiting transformers employing BSCCO cylinders do not return to the normal operation in the time required. For the recovery even with a non-current pause in the circuit, the cylinders are needed with the critical current density by an order higher than the existed ones.Comment: 15 pages, 5 figure

Personal hygiene innovations: clinical laboratory characteristics of a new calcium hydroxylapatite toothpaste

This paper presents the analysis of clinical laboratory and organoleptic properties of the new treatment and prophylactic toothpaste Dentaswiss Extra Whitening along with effects of its components on saliva and oral conditions. The study was carried out by the Ural State Medical University Department of Therapeutic Dentistry/ Propedeutics of Dental Decease and Department of General Chemistry along with the Ural Federal University Department of Control Systems Modeling on 30 volunteers from the students of the Faculty of Dentistry (average patient age was 20.6±1.76 years). The results of the course of Dentaswiss Extra Whitening toothpaste applied during 14 days demonstrated significant clinical effects with high cleansing (hygiene index reduction was 71%) and antiinflammatory capacity (PMA reduction was 72 %). The laboratory study data on physicochemical properties showed improved remineralizing properties of mixed saliva and tooth enamel restoration during the course of Dentaswiss Extra Whitening toothpaste. The organoleptic properties of the tested toothpaste were highly evaluated by the study participants for all parameters (scored 9.92±0.10).В статье представлена оценка клинико-лабораторных и органолептических характеристик новой лечебно-профилактической зубной пасты Dentaswiss Extra Whitening и воздействия ее компонентов на слюну и состояние полости рта. Исследование проведено на кафедре терапевтической стоматологии и пропедевтики стоматологических заболеваний, на кафедре общей химии ФГБОУ ВО УГМУ Минздрава России и на кафедре моделирования управляемых систем ФГАОУ ВО УрФУ у 30 пациентов-добровольцев из числа студентов стоматологического факультета (средний возраст пациентов составил 20,6±1,76 года). Результаты курсового применения ЗП Dentaswiss Extra Whitening на протяжении 14 дней выявили значительный клинический эффект с высоким очищающим (редукция ИГ — 71%) и противовоспалительным действием (редукция индекса РМА — 72%). Данные лабораторного исследования физико-химических свойств показали увеличение реминерализующих свойств смешанной слюны и восстановление эмали зубов при курсовом применении ЗП Dentaswiss Extra Whitening. Органолептические свойства исследуемой зубной пасты высоко оценены участниками исследования по всем параметрам (9,92±0,10 балла)

Hofmeister effect in ion transport: reversible binding of halide anions to the roflamycoin channel.

We have studied the anion-dependent gating of roflamycoin ion channels using spectral analysis of noise in currents through multichannel planar lipid bilayers. We have found that in addition to low frequency current fluctuations that may be attributed to channel switching between open and closed conformations, roflamycoin channels exhibit a pronounced higher frequency noise indicating that the open channel conductance has substates with short lifetimes. This noise is well described by a Lorentzian spectrum component with a characteristic cutoff frequency that depends on the type of halide anions according to their position in the Hofmeister series. It is suggested that transitions between the substates correspond to a reversible ionization of the channel by a penetrating anion that binds to the channel structure, more chaotropic anions being bound for longer times. Within a framework of a two-substate model, the duration of the substate with reduced electrostatic barrier for cation current varies exponentially with anion electron polarizability. This explains two features of the roflamycoin channel reported earlier: the increase in apparent single-channel conductance along the series F- < Cl- < Br- < I- and the reverse of channel selectivity from anionic for KF to cationic for KI

Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80°C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating affected skeletal muscles

Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80°C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating affected skeletal muscles

Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

Experiments were conducted on male mice of the C57BL/6J for full genome study of gene expression. The animals were divided into two groups.The 'flight group' mice that had spent 30 days in space and the 'control group' mice that stayed on Earth. The spinal cord of the 'flight' group mice as extracted 14h following the biosatellite's landing. The spinal cord of the control group mice was extracted at the same time.Then the lumbar spinal cord was frozen in liquid nitrogen and kept until the beginning of the study at -80°C. Quality control of the feature was performed using the settings recommended by Agilent Technologies. Signal background was subtracted and the signal intensity of each gene was globally normalized using locally weighted scatterplot smoothing. The analysis suggests that various functional groups of genes in different types of cells in spinal cord may be involved in the development of HLS and some changes in gene expression certainly happened in motor neurons innervating affected skeletal muscles

Factors predicting the outcomes of removal of corticotropinom in Cushing's disease

Background. Cushings disease (CD) is the heavy disease of the hypothalamic-pituitary-adrenal axis. The cause of the disease is pituitary adenoma (corticotropinoma). Hypersecretion of ACTH by a pituitary adenoma leads to increased secretion of cortisol by the adrenal cortex and the development of total endogenous hypercortisolism. The gold standard treatment for this disease is surgical removal of corticotropinoma. However, adenomectomy is not effective in all cases and approximately 20% of cases after the radical treatment fails to achieve remission of the disease and then comes the relapse. Aims. The aim of our work was to evaluate the factors influencing the result of adenomectomy in patients with the Cushings disease and identification of recurrence predictors. Materials and methods. The study included 84 patients (80 women and 4 men), aged 18 to 58 years with a Cushings disease (CD) before and after the transnasal adenomectomy. The observation period from 3 to 15 years, starting in 2001 (average 9.0 years). There was a retrospective evaluation of clinical and hormonal (ACTH and cortisol) factors before surgery and 13 days and 813 days after surgery. Results. Remission of the disease developed in 54 (64.3%) of the 84 patients with CD after transnasal adenomectomy. Surgical treatment was not effective in 30 patients (35.7%), and they had a repeat adenomectomy. According MRI of the brain among patients with remission of the disease was more frequently found pituitary microadenomas (54%), compared with patients in whom operation was not effective, and MRI were more frequent in macroadenomas (63%). All patients with CD regardless of the outcome of neurosurgical operations was observed a significant decrease of cortisol and ACTH in the early postoperative period. Conclusions. Factors of efficiency of adenomectomy and long-term remission in patients with CD is the levels of morning cortisol less than 100 nmol/l and ACTH of less than 10,0 pg/ml in the early postoperative period (113 days). The presence of adrenal insufficiency after adenomectomy is a predictor of the effectiveness of the operation, but does not exclude the likelihood of relapse