29 research outputs found
Association between Variations in Cell Cycle Genes and Idiopathic Pulmonary Fibrosis
Idiopathic pulmonary fibrosis (IPF) is a devastating and progressive lung disease. Its aetiology is thought to involve damage to the epithelium and abnormal repair. Alveolar epithelial cells near areas of remodelling show an increased expression of proapoptotic molecules. Therefore, we investigated the role of genes involved in cell cycle control in IPF. Genotypes for five single nucleotide polymorphisms (SNPs) in the tumour protein 53 (TP53) gene and four SNPs in cyclin-dependent kinase inhibitor 1A (CDKN1A), the gene encoding p21, were determined in 77 IPF patients and 353 controls. In peripheral blood mononuclear cells (PBMC) from 16 healthy controls mRNA expression of TP53 and CDKN1A was determined
Environmental and Climatic Determinants of Molecular Diversity and Genetic Population Structure in a Coenagrionid Damselfly
Identifying environmental factors that structure intraspecific genetic diversity
is of interest for both habitat preservation and biodiversity conservation.
Recent advances in statistical and geographical genetics make it possible to
investigate how environmental factors affect geographic organisation and
population structure of molecular genetic diversity within species. Here we
present a study on a common and wide ranging insect, the blue tailed damselfly
Ischnuraelegans, which has been the target of many
ecological and evolutionary studies. We addressed the following questions: (i)
Is the population structure affected by longitudinal or latitudinal gradients?;
(ii) Do geographic boundaries limit gene flow?; (iii) Does geographic distance
affect connectivity and is there a signature of past bottlenecks?; (iv) Is there
evidence of a recent range expansion and (vi) what is the effect of geography
and climatic factors on population structure? We found low to moderate genetic
sub-structuring between populations (mean
FST = 0.06,
Dest = 0.12), and an effect of longitude, but
not latitude, on genetic diversity. No significant effects of geographic
boundaries (e.g. water bodies) were found. FST-and
Dest-values increased with geographic distance; however, there was no
evidence for recent bottlenecks. Finally, we did not detect any molecular
signatures of range expansions or an effect of geographic suitability, although
local precipitation had a strong effect on genetic differentiation. The
population structure of this small insect has probably been shaped by ecological
factors that are correlated with longitudinal gradients, geographic distances,
and local precipitation. The relatively weak global population structure and
high degree of genetic variation within populations suggest that I.
elegans has high dispersal ability, which is consistent with this
species being an effective and early coloniser of new habitats
Somatically ill persons’ self-nominated quality of life domains: review of the literature and guidelines for future studies
OBJECTIVE: To review which domains somatically ill persons nominate as constituting their QoL. Specific objective is to examine whether the method of enquiry affect these domains. METHODS: We conducted two literature searches in the databases PubMed/Medline, CINAHL and Psychinfo for qualitative studies examining patients' self-defined QoL domains using (1) SEIQoL and (2) study-specific questions. For each database, two researchers independently assessed the eligibility of the retrieved abstracts and three researchers subsequently classified all QoL domains. RESULTS: Thirty-six eligible papers were identified: 27 studies using the SEIQoL, and nine presenting data derived from study-specific questions. The influence of the method of enquiry on patients' self-nominated QoL domains appears limited: most domains were presented in both types of studies, albeit with different frequencies. CONCLUSIONS: This review provides a comprehensive overview of somatically ill persons' self-nominated QoL domains. However, limitations inherent to reviewing qualitative studies (e.g., the varying level of abstraction of patients' self-defined QoL domains), limitations of the included studies and limitations inherent to the review process, hinder cross-study comparisons. Therefore, we provide guidelines to address shortcomings of qualitative reports amenable to improvement and to stimulate further improvement of conducting and reporting qualitative research aimed at exploring respondents' self-nominated QoL domains