Unusual histology in clear cell renal cell carcinoma from a COVID-19 patient.

(A) The basic histopathological pattern of the cancer case was clear cell renal cell carcinoma. (B) In about 60% of the tumor tissue extensive areas of necrosis could be seen. (C and D) Areas displaying very unusual features where the cancer cells had coalesced into syncytial, multinuclear and discohesive cancer cells with fusion of the lipid laded cytoplasms. All images were stained by Hematoxylin/Eosin, Scale bars in A-C: 100 μm, in D: 200 μm. (TIF)</p

Clinical characteristics of the patients included in the study.

(DOCX)</p

Assessment of protein expression of ACE2, TMPRSS2 and NRP1 in clear cell, papillary and chromophobe renal cell carcinoma.

Assessment of protein expression of ACE2, TMPRSS2 and NRP1 in clear cell, papillary and chromophobe renal cell carcinoma.</p

Uncropped western blot.

(ZIP)</p

SARS-CoV-2 virus replicates in cultured renal cell carcinoma cells and causes a distinct virus cytopathogenic effect.

(A) Negative strand qPCR analysis of SARS-CoV-2 viral RNA copy number at 24h, 48h and 72h post-infection in primary normal tubular epithelial cells, CCRCC cells, PRCC cells and the renal cell carcinoma cell line RCC4. (B) Representative images of virus cytopathogenic effect (CPE) for normal (NKE863), clear cell renal cell carcinoma (CCRCC) (CCRCC863) and papillary renal cell carcinoma (PRCC) (PRCC545) at 48 hours after infection. The CPE causes rounding up of cells, followed by detachment from the growth surface. Mock is control. Scale bar = 200μm.</p