44 research outputs found
MassIVE MSV000089779 - Nanospectral data of amyloid beta 1-42 and scrambled amyloid beta 1-42
Direct Measurements of the Size and Correlations between Single Ions Impelled through a Sub-Nanometer-Diameter Pore
Direct and Simultaneous Force and Current Measurements of Single-Stranded DNA in Synthetic Nanopores
Direct, Concurrent Measurements of the Forces and Currents Affecting DNA in a Nanopore with Comparable Topography
We report direct, concurrent measurements of the forces and currents associated with the translocation of a single-stranded DNA molecule tethered to the tip of an atomic force microscope (AFM) cantilever through synthetic pores with topagraphies comparable to the DNA. These measurements were performed to gauge the signal available for sequencing and the electric force required to impel a single molecule through synthetic nanopores ranging from 1.0 to 3.5 nm in diameter in silicon nitride membranes 6–10 nm thick. The measurements revealed that a molecule can slide relatively frictionlessly through a pore, but regular fluctuations are observed intermittently in the force (and the current) every 0.35–0.72 nm, which are attributed to individual nucleotides translating through the nanopore in a turnstile-like motion