4 research outputs found
A cDNA microarray approach to decipher sunflower (Helianthus annuus) responses to the necrotrophic fungus Phoma macdonaldii
To identify the genes involved in the partial resistance of sunflower (Helianthus annuus) to the necrotrophic fungus Phoma macdonaldii, we developed a 1000âelement cDNA microarray containing carefully chosen genes putatively involved in primary metabolic pathways, signal transduction and biotic stress responses. A twoâpass general linear model was used to normalize the data and then to detect differentially expressed genes. This method allowed us to identify 38 genes differentially expressed among genotypes, treatments and times, mainly belonging to plant defense, signaling pathways and amino acid metabolism. Based on a set of genes whose differential expression was highly significant, we propose a model in which negative regulation of a dualâspecificity MAPK phosphatase could be implicated in sunflower defense mechanisms against the pathogen. The resulting activation of the MAP kinase cascade could subsequently trigger defense responses (e.g. thaumatin biosynthesis and phenylalanine ammonia lyase activation), under the control of transcription factors belonging to MYB and WRKY families. Concurrently, the activation of protein phosphatase 2A (PP2A), which is implicated in cell death inhibition, could limit pathogen development. The results reported here provide a valuable first step towards the understanding and analysis of the P. macdonaldiiâsunflower interaction
Phoma et dessÚchement précoce du tournesol
Cet ouvrage fait le point sur le phoma et le dessĂšchement prĂ©coce du tournesol : connaissances actuelles sur le champignon et la maladie, mĂ©thodes de contamination, impact de la conduite de culture, rĂ©sistance gĂ©nĂ©tique du tournesol au phoma, mĂ©thodes de lutte. Il fait suite Ă de nombreux travaux de recherche, menĂ©s principalement en France depuis 5 ans dans le cadre de lâUMT Tournesol Ă Toulouse par les Ă©quipes du CETIOM, de lâEI Purpan, de lâENSAT et de lâINRA
Phoma et dessÚchement précoce du tournesol
Cet ouvrage fait le point sur le phoma et le dessĂšchement prĂ©coce du tournesol : connaissances actuelles sur le champignon et la maladie, mĂ©thodes de contamination, impact de la conduite de culture, rĂ©sistance gĂ©nĂ©tique du tournesol au phoma, mĂ©thodes de lutte. Il fait suite Ă de nombreux travaux de recherche, menĂ©s principalement en France depuis 5 ans dans le cadre de lâUMT Tournesol Ă Toulouse par les Ă©quipes du CETIOM, de lâEI Purpan, de lâENSAT et de lâINRA