Targeting EZH2-mediated methylation of H3K27 inhibits proliferation and migration of Synovial Sarcoma in vitro.

Synovial sarcoma is an aggressive soft tissue sarcoma genetically defined by the fusion oncogene SS18-SSX. It is hypothesized that either SS18-SSX disrupts SWI/SNF complex inhibition of the polycomb complex 2 (PRC2) methyltransferase Enhancer of Zeste Homologue 2 (EZH2), or that SS18-SSX is able to directly recruit PRC2 to aberrantly silence target genes. This is of potential therapeutic value as several EZH2 small molecule inhibitors are entering early phase clinical trials. In this study, we first confirmed EZH2 expression in the 76% of human synovial sarcoma samples. We subsequently investigated EZH2 as a therapeutic target in synovial sarcoma in vitro. Knockdown of EZH2 by shRNA or siRNA resulted in inhibition of cell growth and migration across a series of synovial sarcoma cell lines. The EZH2 selective small-molecule inhibitor EPZ005687 similarly suppressed cell proliferation and migration. These data support the hypothesis that targeting EZH2 may be a promising therapeutic strategy in the treatment of synovial sarcoma; clinical trials are initiating enrollment currently

Eleven New γ Doradus Stars

We present new high-dispersion spectroscopic and precise photometric observations to identify 11 new γ Doradus variables. Seven of these new γ Doradus stars appear to be single, three are primaries of single-lined binaries, and one has two distant visual companions; none are double-lined or close visual binaries. Several of the stars show spectroscopic line-profile and low-amplitude radial velocity variability indicative of pulsation. All 11 stars are photometrically variable with amplitudes between 8 and 93 mmag in Johnson B and periods between 0.398 and 2.454 days. One star is monoperiodic; the rest have between two and five independent periods. The variability at all periods approximates a sinusoid, although three of the stars exhibit cycle-to-cycle variation in the level of maximum brightness, similar to the Blazhko effect observed in some RR Lyrae stars. We provide a new tabulation of all 54 γ Doradus stars confirmed to date and list some of their properties. All are dwarfs or subgiants and lie within a well-defined region of the H-R diagram that overlaps the cool edge of the δ Scuti instability strip. Four of the new γ Doradus variables from this paper also lie within the δ Scuti instability strip but do not exhibit the additional higher frequency variability typical of δ Scuti stars. The variability type of several of these stars given in the General Catalog of Variable Stars and in SIMBAD should now be revised

HD 207651: A Triple System with δ Scuti and Ellipsoidal Variations But No γ Doradus Pulsations

We examine HD 207651 as a possible example of a star exhibiting both γ Doradus and δ Scuti type pulsations. We find photometric periods of 0.06479 and 0.06337 days with peak-to-peak amplitudes in Johnson B of 21 and 13 mmag, respectively, clearly indicating δ Scuti pulsations. Additional light variation with a period of 0.73540 days and an even larger amplitude of 31 mmag is within the range of γ Doradus pulsation periods but results instead from the ellipticity effect. HD 207651 has a composite spectrum with a weak, narrow absorption line superposed near the center of each broad metal line. The broad-lined component is the primary of a short-period, single-lined binary, which has a period of 1.4708 days, twice the period of the ellipsoidal variations seen in the photometry. We determine the primary to be an A8 giant and estimate the unseen secondary of the short-period binary to be a mid-M dwarf. The narrow-lined star, an F7: dwarf, shows velocity variability with a period of months or perhaps years. It is thus a more distant companion to the binary, making HD 207651 a triple system. All light variations come from the A8 giant primary star. Since the 0.73540 day variation results from the ellipticity effect, HD 207651 is not an example of a star that exhibits both δ Scuti and γ Doradus pulsations. The growing number of confirmed γ Doradus stars that also occur within the δ Scuti instability strip but fail to show additional δ Scuti variability makes it increasingly unlikely that the two types of pulsation can coexist in the same star

Male-killing Wolbachia do not protect Drosophila bifasciata against viral infection.

BACKGROUND: Insect symbionts employ multiple strategies to enhance their spread through populations, and some play a dual role as both a mutualist and a reproductive manipulator. It has recently been found that this is the case for some strains of Wolbachia, which both cause cytoplasmic incompatibility and protect their hosts against viruses. Here, we carry out the first test as to whether a male-killing strain of Wolbachia also provides a direct benefit to its host by providing antiviral protection to its host Drosophila bifasciata. We infected flies with two positive sense RNA viruses known to replicate in a range of Drosophila species (Drosophila C virus and Flock House virus) and measure the rate of death in Wolbachia positive and negative host lines with the same genetic background. RESULTS: Both viruses caused considerable mortality to D. bifasciata flies, with Drosophila C virus killing 43% more flies than the uninfected controls and Flock House virus killing 78% more flies than the uninfected controls. However, viral induced mortality was unaffected by the presence of Wolbachia. CONCLUSION: In the first male-killing Wolbachia strain tested for antiviral effects, we found no evidence that it conferred protection against two RNA viruses. We show that although antiviral resistance is widespread across the Wolbachia phylogeny, the trait seems to have been lost or gained along some lineages. We discuss the potential mechanisms of this, and can seemingly discount protection against these viruses as a reason why this symbiont has spread through Drosophila populations.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are