Can Fur Traders Have Feelings? Sentiment in Samuel Hearne’s Journey to the Northern Ocean (1795)

Samuel Hearne’s lurid and sentimental recounting of the “Bloody Falls” massacre in Journey to the Northern Ocean (1795) has raised doubts as to whether the fur trader was, in fact, the account’s author. However, historical documents indicate that Hearne was capable of writing at the level of complexity and correctness seen in this passage: Hearne’s letters to the London directors of the HBC significantly differ stylistically from his post journals; Hearne’s family, education, and interests are consistent with those of an aspiring writer; and most importantly, the account of the massacre is consistent with ideas, analogous incidents, emotional expressions, and the narrating persona found throughout the Journey, all of which suggest Hearne’s familiarity and engagement with the influential sentimentalist thought of his time

The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation

It has been reported that nontransformed mammalian cells become arrested during G1 in the absence of centrioles (Hinchcliffe, E., F. Miller, M. Cham, A. Khodjakov, and G. Sluder. 2001. Science. 291:1547–1550). Here, we show that removal of resident centrioles (by laser ablation or needle microsurgery) does not impede cell cycle progression in HeLa cells. HeLa cells born without centrosomes, later, assemble a variable number of centrioles de novo. Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase. These, initially amorphous “precentrioles” become morphologically recognizable centrioles before mitosis. De novo–assembled centrioles mature (i.e., gain abilities to organize microtubules and replicate) in the next cell cycle. This maturation is not simply a time-dependent phenomenon, because de novo–formed centrioles do not mature if they are assembled in S phase–arrested cells. By selectively ablating only one centriole at a time, we find that the presence of a single centriole inhibits the assembly of additional centrioles, indicating that centrioles have an activity that suppresses the de novo pathway