Actor–Networks, Farmer Decisions, and Identity

Climate change and industrial agricultural practices pose threats to the future of Kansas agriculture. To inform the debate about sustainable agriculture that must soon occur, we seek to illuminate the factors involved in the decision making of farmers in Kansas. Drawing from Actor–Network Theory, we consider how farmers’ participation in the industrial agricultural network shapes their decisions, defines the types of knowledge and skills that are valued by farmers and others in the network, transforms what it means to be a Kansas grain farmer, and entrenches unsustainable production practices

Regulating the Ogallala: Paradox and Ambiguity in Western Kansas

Purpose: To illuminate the underlying logic of western Kansas farmers’ decisions to irrigate at unsustainable rates and the state’s regulatory policies and practices that enable depletion of the Ogallala aquifer. Methodology/approach: Ethnographic interviewing of 39 western Kansas farmers, state water management personnel, and archival research. Findings: Farmers occupy an ambiguous position as petty capitalists who focus attention on their own farms with seasonal planning horizons, and they hold a view of “good stewardship” that melds economic and noneconomic considerations, and that provides a rationale for unsustainable irrigation practices. The state resolves the contradiction between the finite groundwater resource and ideological commitments to economic growth by devolving responsibility for water management to groundwater users. Research Limitations/Implications: While the small sample size is likely to be representative of the larger pool of irrigators, further research with other farmers representative of the region will be necessary to verify findings. Social implications: Depletion of the Ogallala aquifer contributes to farm consolidation and community decline, and the ecological costs will leave future farmers and remaining communities without the benefits of groundwater. Western Kansas will likely have to revert to a system of dryland farming

Whole-cell analysis of low-density lipoprotein uptake by macrophages using STEM tomography

Nanoparticles of heavy materials such as gold can be used as markers in quantitative electron microscopic studies of protein distributions in cells with nanometer spatial resolution. Studying nanoparticles within the context of cells is also relevant for nanotoxicological research. Here, we report a method to quantify the locations and the number of nanoparticles, and of clusters of nanoparticles inside whole eukaryotic cells in three dimensions using scanning transmission electron microscopy (STEM) tomography. Whole-mount fixed cellular samples were prepared, avoiding sectioning or slicing. The level of membrane staining was kept much lower than is common practice in transmission electron microscopy (TEM), such that the nanoparticles could be detected throughout the entire cellular thickness. Tilt-series were recorded with a limited tilt-range of 80u thereby preventing excessive beam broadening occurring at higher tilt angles. The 3D locations of the nanoparticles were nevertheless determined with high precision using computation. The obtained information differed from that obtained with conventional TEM tomography data since the nanoparticles were highlighted while only faint contrast was obtained on the cellular material. Similar as in fluorescence microscopy, a particular set of labels can be studied. This method was applied to study the fate of sequentially up-taken low-density lipoprotein (LDL) conjugated to gold nanoparticles in macrophages. Analysis of a 3D reconstruction revealed that newly up-taken LDL-gold was delivered to lysosomes containing previously up-taken LDL-gold thereby forming onion-like clusters

Исследование механизмов синтеза керамических материалов нового поколения на основе тетрарных оксикарбонитридных фаз циркония и титана

Работа посвящена исследованию механизмов синтеза керамических материалов нового поколения на основе тетрарных оксикарбонитридных фаз циркония и титана. Были определены плотность спечённых образцов, измерены модуль упругости, нанотвердость и предел прочности с использованием методики наноиндентрования и скрэч-тестинга, исследованы фазовый состав и микроструктура спеченных образцов. В результате выполнения работ методом горячего прессования получена высокомодульная прочная оксинитридная керамика.The work is devoted to the study of mechanisms of synthesis of new generation ceramic materials based on tetrad oxycarbonitride phases of zirconium and titanium. The density of sintered samples was determined, the modulus of elasticity, nanohardness and ultimate strength were measured using the technique of nanoindentation and scratch testing, and the phase composition and microstructure of sintered samples were studied. As a result of hot pressing, high-modulus, strong oxynitride ceramics were obtained

Microsomal triglyceride transfer protein expression in adipocytes: A new component in fat metabolism

AbstractMicrosomal triglyceride transfer protein (MTP) is a carrier of triglyceride essential for the assembly of apolipoprotein (apo)B-containing lipoproteins by the liver and the small intestine. Its role in triglyceride transfer in tissues that do not secrete lipoproteins has not been explored. In particular, MTP would seem to be a candidate for a role in triglyceride metabolism within the adipocyte. To test this hypothesis, we probed adipocytes for the presence of MTP. Immunohistochemical and biochemical studies demonstrate MTP in adipocytes from brown and white fat depots of mice and human, as well as in 3T3-L1 cells. Confocal microscopy revealed MTP throughout 3T3 cells; however, MTP fluorescence was prominent in juxtanuclear areas. In differentiated 3T3 cells MTP fluorescence was very striking around lipid droplets. In vitro lipid transfer assays demonstrated the presence of triglyceride transfer activity within microsomal fractions isolated from rat adipose tissue. In addition, quantitative rtPCR studies showed that MTP expression in mouse white fat depots was approximately 1% of MTP expression in mouse liver. MTP mRNA in differentiated 3T3 cells was approximately 13% of liver expression. Our results provide unequivocal evidence for the presence of MTP in adipocytes and present new possibilities for defining the mechanisms by which triglyceride is stored and/or hydrolyzed and mobilized

Relating agriculture, energy, and water decisions to farm incomes and climate projections using two freeware programs, FEWCalc and DSSAT

Context: The larger scale perspective of Integrated Assessment (IA) and smaller scale perspective of Impacts, Adaptation, and Vulnerability (IAV) need to be bridged to design long-term solutions to agricultural problems that threaten agricultural production, rural economic viability, and global food supplies. FEWCalc (Food-Energy-Water Calculator) is a new freeware, agent-based model with the novel ability to project farm incomes based on crop selection, irrigation practices, groundwater availability, renewable energy investment, and historical and projected environmental conditions. FEWCalc is used to analyze the interrelated food, energy, water, and climate systems of Finney County, Kansas to evaluate consequences of choices currently available to farmers and resource managers. Objective: This article aims to evaluate local farmer choices of crops and renewable energy investment in the face of water resource limitations and global climate change. Metrics of the analysis include agricultural and renewable-energy production, farm income, and water availability and quality. The intended audience includes farmers, resource managers, and scientists focusing on food, energy, and water systems. Methods: Data derived from publicly available sources are used to support user-specified FEWCalc input values. DSSAT (Decision Support System for Agrotechnology Transfer) with added arid-region dynamics is used to obtain simulated crop production and irrigation water demand for FEWCalc. Here, FEWCalc is used to simulate agricultural and energy production and farm income based on continuation of recent ranges of crop prices, farm expenses, and crop insurance; continuation of recent renewable-energy economics and government incentives; one of four climate scenarios, including General Circulation Model projections for Representative Concentration Pathway 8.5; and groundwater-supported irrigation and its limitations. Results and Conclusions: A 50-year (2018-2067) climate and groundwater availability projection process indicates possible trends of future crop yield, water utility, and farm income. The simulation during more wet years produces high crop production and slower depletion of groundwater, as expected. However, surprisingly, the simulations suggest that only the Drier Future scenario is commercially profitable, and this is because of reduced expenses for dryland farming. Although simulated income losses due to low crop production are ameliorated by the energy sector income and crop insurance, the simulation under climate change still produces the worst annual total income. Significance: FEWCalc addresses scientific, communication, and educational gaps between global- and local-scale FEW research communities and local stakeholders, affected by food, energy, water systems and their interactions by relating near-term choices to near- and long-term consequences. This analysis is needed to craft a more advantageous future

Size- and charge-dependent non-specific uptake of PEGylated nanoparticles by macrophages

The assessment of macrophage response to nanoparticles is a central component in the evaluation of new nanoparticle designs for future in vivo application. This work investigates which feature, nanoparticle size or charge, is more predictive of non-specific uptake of nanoparticles by macrophages. This was investigated by synthesizing a library of polymer-coated iron oxide micelles, spanning a range of 30–100 nm in diameter and −23 mV to +9 mV, and measuring internalization into macrophages in vitro. Nanoparticle size and charge both contributed towards non-specific uptake, but within the ranges investigated, size appears to be a more dominant predictor of uptake. Based on these results, a protease-responsive nanoparticle was synthesized, displaying a matrix metalloproteinase-9 (MMP-9)-cleavable polymeric corona. These nanoparticles are able to respond to MMP-9 activity through the shedding of 10–20 nm of hydrodynamic diameter. This MMP-9-triggered decrease in nanoparticle size also led to up to a six-fold decrease in nanoparticle internalization by macrophages and is observable by T2-weighted magnetic resonance imaging. These findings guide the design of imaging or therapeutic nanoparticles for in vivo targeting of macrophage activity in pathologic states

Novel Changes in Discoidal High Density Lipoprotein Morphology: A Molecular Dynamics Study

AbstractApoA-I is a uniquely flexible lipid-scavenging protein capable of incorporating phospholipids into stable particles. Here we report molecular dynamics simulations on a series of progressively smaller discoidal high density lipoprotein particles produced by incremental removal of palmitoyloleoylphosphatidylcholine via four different pathways. The starting model contained 160 palmitoyloleoylphosphatidylcholines and a belt of two antiparallel amphipathic helical lipid-associating domains of apolipoprotein (apo) A-I. The results are particularly compelling. After a few nanoseconds of molecular dynamics simulation, independent of the starting particle and method of size reduction, all simulated double belts of the four lipidated apoA-I particles have helical domains that impressively approximate the x-ray crystal structure of lipid-free apoA-I, particularly between residues 88 and 186. These results provide atomic resolution models for two of the particles produced by in vitro reconstitution of nascent high density lipoprotein particles. These particles, measuring 95Å and 78Å by nondenaturing gradient gel electrophoresis, correspond in composition and in size/shape (by negative stain electron microscopy) to the simulated particles with molar ratios of 100:2 and 50:2, respectively. The lipids of the 100:2 particle family form minimal surfaces at their monolayer-monolayer interface, whereas the 50:2 particle family displays a lipid pocket capable of binding a dynamic range of phospholipid molecules

Towards a framework for critical citizenship education

Increasingly countries around the world are promoting forms of "critical" citizenship in the planned curricula of schools. However, the intended meaning behind this term varies markedly and can range from a set of creative and technical skills under the label "critical thinking" to a desire to encourage engagement, action and political emancipation, often labelled "critical pedagogy". This paper distinguishes these manifestations of the "critical" and, based on an analysis of the prevailing models of critical pedagogy and citizenship education, develops a conceptual framework for analysing and comparing the nature of critical citizenship

Evidence from GC-TRFLP that Bacterial Communities in Soil Are Lognormally Distributed

The Species Abundance Distribution (SAD) is a fundamental property of ecological communities and the form and formation of SADs have been examined for a wide range of communities including those of microorganisms. Progress in understanding microbial SADs, however, has been limited by the remarkable diversity and vast size of microbial communities. As a result, few microbial systems have been sampled with sufficient depth to generate reliable estimates of the community SAD. We have used a novel approach to characterize the SAD of bacterial communities by coupling genomic DNA fractionation with analysis of terminal restriction fragment length polymorphisms (GC-TRFLP). Examination of a soil microbial community through GC-TRFLP revealed 731 bacterial operational taxonomic units (OTUs) that followed a lognormal distribution. To recover the same 731 OTUs through analysis of DNA sequence data is estimated to require analysis of 86,264 16S rRNA sequences. The approach is examined and validated through construction and analysis of simulated microbial communities in silico. Additional simulations performed to assess the potential effects of PCR bias show that biased amplification can cause a community whose distribution follows a power-law function to appear lognormally distributed. We also show that TRFLP analysis, in contrast to GC-TRFLP, is not able to effectively distinguish between competing SAD models. Our analysis supports use of the lognormal as the null distribution for studying the SAD of bacterial communities as for plant and animal communities