2 research outputs found
Insulin receptor (IR) expression in human trophoblasts of recurrent pregnancy loss (RPL)
Purpose: Insulin and glucose pathways play a key role to fetal viability and growth. The focus of the study is to investigate the potential differences of immunohistochemical expression of IR in trophoblastic and decidual cells between women who had recurrent pregnancy loss and women that underwent an abortion. Materials and methods: Trophoblastic and decidual tissues from fifty (50) women with elective abortion used as control group and from fifty (50) women with recurrent miscarriages were collected during gestational weeks 6 to 12. IR antibodies were used as immunohistochemical staining markers. Nuclear and cytoplasmic expression was evaluated. Results: No IR immunohistochemical expression was detected in both trophoblastic cells of the implantation site and deciduas basalis of the two study groups. Conclusion: The effort made to enhance our knowledge on the physiology and histology of IR expression in connection with pregnancy was halted because the results were inconclusive. While studying, though, the correlation of recurrent miscarriage with IR expression, it became evident that a lot of hormones and pathways form the weave of gestational pathology and its delicate harmony. Every piece of knowledge may clarify this still obscure field
Low expression of Progesterone Receptor A in intermediate trophoblast of miscarriages
Summary. Objective: To examine the potential
differences in the expression of Progesterone Receptor A
and Estrogen Receptor A in intermediate trophoblastic
cells at the implantation site in elective abortions and
miscarriages by immunohistochemistry.
Study Design: Twenty two (22) samples of
miscarriages and eighteen (18) samples of elective
abortions were obtained during gestational weeks 6 to
12. Monoclonal antibodies against Cytokeratin 7 and
prolactin were used to help discriminate between
trophoblastic and decidual cells at the feto-maternal
interface on formalin-fixed paraffin-embedded sections.
Samples were then stained with ERA and PRA
antibodies. Nuclear expression was considered positive.
Staining intensity was measured according to a 4 grade
scale. Statistical analysis of the results was performed
using the Mann-Whitney test and the Wilcoxon signed
rank test.
Results: PRA expression in intermediate
trophoblastic cells was significantly higher in elective
abortions (control group) compared to miscarriages.
ERA expression was uniformly negative in both groups.
Conclusion: PRA expression is significantly lower
in intermediate trophoblastic cells of miscarriages
compared to elective abortion pregnancies. Although
this could be solely a result of a secondary event, it is
still an important finding in the effort to unravel the
complex molecular pathobiology of spontaneous
abortions