7,333 research outputs found
Pichia anomala:cell physiology and biotechnology relative to other yeasts
Pichia anomala is a most interesting yeast species, from a number of environmental, industrial and medical aspects. This yeast has been isolated from very diverse natural habitats (e.g. in foods, insects, wastewaters etc.) and it also exhibits wide metabolic and physiological diversity. Some of the activities of P. anomala, particularly its antimicrobial action, make it a very attractive organism for biological control applications in the agri-food sectors of industry. Being a ‘robust’ organism, it additionally has potential to be exploited in bioremediation of environmental pollutants. This paper provides an overview of cell physiological characteristics (growth, metabolism, stress responses) and biotechnological potential (e.g. as a novel biocontrol agent) of P. anomala and compares such properties with other yeast species, notably Saccharomyces cerevisiae, which remains the most exploited industrial microorganism. We await further basic knowledge of P. anomala cell physiology and genetics prior to its fuller commercial exploitation, but the exciting biotechnological potential of this yeast is highlighted in this paper
125th anniversary review: fuel alcohol: current production and future challenges
Global research and industrial development of liquid transportation biofuels are moving at a rapid pace. This is mainly due to the significant roles played by biofuels in decarbonising our future energy needs, since they act to mitigate the deleterious impacts of greenhouse gas emissions to the atmosphere that are contributors of climate change. Governmental obligations and international directives that mandate the blending of biofuels in petrol and diesel are also acting as great stimuli to this expanding industrial sector. Currently, the predominant liquid biofuel is bioethanol (fuel alcohol) and its worldwide production is dominated by maize-based and sugar cane-based processes in North and South America, respectively. In Europe, fuel alcohol production employs primarily wheat and sugar beet. Potable distilled spirit production and fuel alcohol processes share many similarities in terms of starch bioconversion, fermentation, distillation and co-product utilisation, but there are some key differences. For example, in certain bioethanol fermentations, it is now possible to yield consistently high ethanol concentrations of ~20% (v/v). Emerging fuel alcohol processes exploit lignocellulosic feedstocks and scientific and technological constraints involved in depolymerising these materials and efficiently fermenting the hydrolysate sugars are being overcome. These so-called secondgeneration fuel alcohol processes are much more environmentally and ethically acceptable compared with exploitation of starch and sugar resources, especially when considering utilisation of residual agricultural biomass and biowastes. This review covers both first and second-generation bioethanol processes with a focus on current challenges and future opportunities of lignocellulose-to-ethanol as this technology moves from demonstration pilot-plants to full-scale industrial facilities
Distributional Measures of Semantic Distance: A Survey
The ability to mimic human notions of semantic distance has widespread
applications. Some measures rely only on raw text (distributional measures) and
some rely on knowledge sources such as WordNet. Although extensive studies have
been performed to compare WordNet-based measures with human judgment, the use
of distributional measures as proxies to estimate semantic distance has
received little attention. Even though they have traditionally performed poorly
when compared to WordNet-based measures, they lay claim to certain uniquely
attractive features, such as their applicability in resource-poor languages and
their ability to mimic both semantic similarity and semantic relatedness.
Therefore, this paper presents a detailed study of distributional measures.
Particular attention is paid to flesh out the strengths and limitations of both
WordNet-based and distributional measures, and how distributional measures of
distance can be brought more in line with human notions of semantic distance.
We conclude with a brief discussion of recent work on hybrid measures
Yeasts
Yeasts are a group of eukaryotic microfungi with a well-defined cell wall whose growth is either entirely unicellular or a combination of hyphal and unicellular reproduction. The approximately 1500 known yeast species belong to two distinct fungal phyla, the Ascomycota and the Basidiomycota. Within each these phyla, yeasts can be found in several subphyla or classes, reflecting the enormous diversity of their evolutionary origins and biochemical properties. In nature, yeasts are found mainly in association with plants or animals but are also present in soil and aquatic environments. Yeasts grow rapidly and have simple nutritional requirements, for which reason they have been used as model systems in biochemistry, genetics and molecular biology. They were the first microorganisms to be domesticated for the production of beer, bread or wine, and they continue to be used for the benefit of humanity in the production of many important health care and industrial commodities, including recombinant proteins, biopharmaceuticals, biocontrol agents and biofuels. The best-known yeast is the species Saccharomyces cerevisiae, which may be regarded as the world’s foremost industrial microbe
Combining motility and bioluminescent signalling aids mate finding in deep-sea fish: a simulation study
We present a model to estimate the mean time required for mate finding among deep-sea fish as a function of motility and the extent of bioluminescent signalling. This model differs from those of previous works in 3 important ways by including (1) sex differences in motility, (2) a maximum detection range of bioluminescent signals derived from a recently published mechanistic model based on physical principles and the physiology of vision, and (3) a novel consideration of the likelihood of individuals passing within detection range only in the interval between flashes and hence, failing to detect the signaller. We argue that the flash rates required for effective detection are low, with rates of less than 1 per minute being entirely plausible, and that predation pressure may further encourage low flash rates. Further, even at high flash frequencies, the energetic cost of bioluminescent signalling is argued to be a trivial fraction of resting metabolic rates. Using empirically derived estimates for parameter values, we estimate that a female will be detected and reached by a male within 2 to 4 h of beginning to signal. Hence, we argue that mate finding may not seriously restrict reproductive success in species that can exploit this signalling system. We further argue that where male motility allows bioluminescent signalling, this may have some advantages over chemical-based signalling. Bioluminescent signalling may, therefore, be more important to mate finding in the deep sea (relative to chemical signals) than some previous works have suggested
Influence of cell surface characteristics on adhesion of Saccharomyces cerevisiae to the biomaterial hydroxylapatite
The influence of the physicochemical properties of biomaterials on microbial cell adhesion is well known, with the extent of adhesion depending on hydrophobicity, surface charge, specific functional groups and acid–base properties. Regarding yeasts, the effect of cell surfaces is often overlooked, despite the fact that generalisations may not be made between closely related strains. The current investigation compared adhesion of three industrially relevant strains of Saccharomyces cerevisiae (M-type, NCYC 1681 and ALY, strains used in production of Scotch whisky, ale and lager, respectively) to the biomaterial hydroxylapatite (HAP). Adhesion of the whisky yeast was greatest, followed by the ale strain, while adhesion of the lager strain was approximately 10-times less. According to microbial adhesion to solvents (MATS) analysis, the ale strain was hydrophobic while the whisky and lager strains were moderately hydrophilic. This contrasted with analyses of water contact angles where all strains were characterised as hydrophilic. All yeast strains were electron donating, with low electron accepting potential, as indicated by both surface energy and MATS analysis. Overall, there was a linear correlation between adhesion to HAP and the overall surface free energy of the yeasts. This is the first time that the relationship between yeast cell surface energy and adherence to a biomaterial has been described
Saccharomyces cerevisiae in the production of fermented beverages
Alcoholic beverages are produced following the fermentation of sugars by yeasts, mainly (but not exclusively) strains of the species, Saccharomyces cerevisiae. The sugary starting materials may emanate from cereal starches (which require enzymatic pre‐hydrolysis) in the case of beers and whiskies, sucrose‐rich plants (molasses or sugar juice from sugarcane) in the case of rums, or from fruits (which do not require pre‐hydrolysis) in the case of wines and brandies. In the presence of sugars, together with other essential nutrients such as amino acids, minerals and vitamins, S. cerevisiae will conduct fermentative metabolism to ethanol and carbon dioxide (as the primary fermentation metabolites) as the cells strive to make energy and regenerate the coenzyme NAD+ under anaerobic conditions. Yeasts will also produce numerous secondary metabolites which act as important beverage flavour congeners, including higher alcohols, esters, carbonyls and sulphur compounds. These are very important in dictating the final flavour and aroma characteristics of beverages such as beer and wine, but also in distilled beverages such as whisky, rum and brandy. Therefore, yeasts are of vital importance in providing the alcohol content and the sensory profiles of beverages. This Introductory Chapter reviews, in general, the growth, physiology and metabolism of S. cerevisiae in alcoholic beverage fermentations
<i>Saccharomyces cerevisiae</i> in the production of whisk(e)y
Whisk(e)y is a major global distilled spirit beverage. Whiskies are produced from cereal starches that are saccharified, fermented and distilled prior to spirit maturation. The strain of Saccharomyces cerevisiae employed in whisky fermentations is crucially important not only in terms of ethanol yields, but also for production of minor yeast metabolites which collectively contribute to development of spirit flavour and aroma characteristics. Distillers must therefore pay very careful attention to the strain of yeast exploited to ensure consistency of fermentation performance and spirit congener profiles. In the Scotch whisky industry, initiatives to address sustainability issues facing the industry (for example, reduced energy and water usage) have resulted in a growing awareness regarding criteria for selecting new distilling yeasts with improved efficiency. For example, there is now a desire for Scotch whisky distilling yeasts to perform under more challenging conditions such as high gravity wort fermentations. This article highlights the important roles of S. cerevisiae strains in whisky production (with particular emphasis on Scotch) and describes key fermentation performance attributes sought in distiller’s yeast, such as high alcohol yields, stress tolerance and desirable congener profiles. We hope that the information herein will be useful for whisky producers and yeast suppliers in selecting new distilling strains of S. cerevisiae, and for the scientific community to stimulate further research in this area
Rigorous bounds on the effective moduli of composites and inhomogeneous bodies with negative-stiffness phases
We review the theoretical bounds on the effective properties of linear
elastic inhomogeneous solids (including composite materials) in the presence of
constituents having non-positive-definite elastic moduli (so-called
negative-stiffness phases). We show that for statically stable bodies the
classical displacement-based variational principles for Dirichlet and Neumann
boundary problems hold but that the dual variational principle for traction
boundary problems does not apply. We illustrate our findings by the example of
a coated spherical inclusion whose stability conditions are obtained from the
variational principles. We further show that the classical Voigt upper bound on
the linear elastic moduli in multi-phase inhomogeneous bodies and composites
applies and that it imposes a stability condition: overall stability requires
that the effective moduli do not surpass the Voigt upper bound. This
particularly implies that, while the geometric constraints among constituents
in a composite can stabilize negative-stiffness phases, the stabilization is
insufficient to allow for extreme overall static elastic moduli (exceeding
those of the constituents). Stronger bounds on the effective elastic moduli of
isotropic composites can be obtained from the Hashin-Shtrikman variational
inequalities, which are also shown to hold in the presence of negative
stiffness
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