Reproductive biotechnology in animal husbandry: Current status and future prospects

To date there is still no optimal biotechnology which ensures maximum preservation of the functional parameters of the spermatozoa from buffaloes, boars and dogs. The aim of this research is to study the biological potential of seminal plasma proteins that are specific only to ejaculates with high cryotolerance and good quality parameters of the spermatozoa. The motility and velocity parameters of the spermatozoa were assessed by computer-assisted sperm analysis. Seminal plasma proteins were separated by size-exclusion liquid chromatography and characterized by polyacrylamide gel electrophoresis and mass spectrometry. Based on the results obtained, sperm diluents and methods for biological evaluation of the fertilization potential of the spermatozoa from buffalo bulls, boars and dogs were created and proposed for practical application

EFECT OF SMOKING ON THE ACCUMULATION OF POLYCYCLIC AROMATIC HIDROCARBONS, IN M. LONGISIMUS DORSI FROM PIGS AND POSSIBILITIES FOR REDUCING THEIR CONTENT

This study deals with the effect of smoking process and the accumulation of toxic polycyclic aromatic hydrocarbons in Musculus Longissimus dorsi from pigs and studies the possibilities to reduce their content. The studies were conducted on two samples boiled-smoked fillet – one salted with brine and added proteincoating, whereas the second one salted with brine and added ascorbic acid.Qualitative identification and quantification of polycyclic aromatic hydrocarbons is done by gas hromotograph Hewlett Packard 5890 with LG 85 B spectrophotometric detector. It was found that the use of proteincoatings, significantly reduces the amount of polycyclic aromatic hydrocarbons in the smoking of Musculus Longissimus dorsi from pigs. The addition of ascorbic acid in conjunction with protein salting coatings technology represents a significant option for reducing the content of the benzo(a)pyrene , which contributes to the safety in the final products. In studies of the samples of proteins and ascorbic acid, the inner layers i.e the muscle tissue was found to contain significantly less amount of benzo(a)pyrene (1,75 ± 0,06 μ/kg) compared with the values found in the surface layers (2,31 ± 0,02 μg/kg)

Funkcionalne osobine sojeva bakterija mlečne kiseline i mikrokoka u sredini sličnoj mesnoj masi sirovih kobasica kao modelu

In this study we investigated the potential probiotic properties of Lactobacillus plantarum L 24 - 2, Lactococcus lactis biovar diacetylactis N 237 and strain Micrococcus sp. It has been found that microbial species exhibited the ability to survive under conditions of high concentrations of bile salts (2.0%) and low pH (2.0). This ability varies for different types, but they will remain until the end of experiments zivotosposobne. This, together with the antimicrobial activity projavljenoj them makes them suitable for the new composition uklucivane the starter culture, which can be effectively used as an antimicrobial barrier to the development of pathogenic bacteria in the manufacture of a crude - product of dried meat.U ovom radu su ispitivana potencijalna probiotska svojstva bakterija Lactobacillus Plantarum soj L 24 - 2, Lactococcus lactis biovar diacetilactis soj N 237 i Micrococcus sp. Utvrđeno je da mikrobne vrste pokazuju sposobnost za preživljavanje pod uslovima visoke koncentracije žučnih soli (2,0%) i niskog pH (2.0). Ova sposobnost varira kod različitih ispitivanih bakterija, ali sve one ostaju životno sposobne do kraja eksperimenata. Ovo, zajedno sa dokazanom antimikrobnom aktivnošću čini ih pogodnim za uključivane u sastav novih starter kultura, koje mogu efikasno poslužiti kao antimikrobna barijera za razvoj patogenih bakterija u proizvodnji sirovih - sušenih proizvoda od mesa