6 research outputs found
Proteomic profile of serum of pregnant women carring a fetus with Down syndrome using nano uplc Q-tof ms/ms technology
<p><b>Introduction:</b> Prenatal diagnosis of Down syndrome (DS) is based on the calculated risk of maternal age, biochemical and ultrasonographic markers and recently by cfDNA. Differences in proteomic profiles may give an opportunity to find new biomarkers.</p> <p><b>Objective:</b> Characterize proteome of serum of mothers carrying DS fetus.</p> <p><b>Material and methods:</b> Blood serum samples of three groups of women were obtained, (a) 10 non-pregnant, (b) 10 pregnant with healthy fetus by ultrasound evaluation, (c) nine pregnant with DS fetus. Sample preparation was as follows: Albumin/IgG depletion, desalting, and trypsin digestion; the process was performed in nanoUPLC MS/MS. Data analysis was made with Mass Lynx 4.1 and ProteinLynx Global Server 3.0, peptide and protein recognition by MASCOT algorithm and UNIPROT-Swissprot database.</p> <p><b>Results:</b> Each group showed different protein profiles. Some proteins were shared between groups. Only sera from pregnant women showed proteins related to immune and clot pathways. Mothers with DS fetus had 42 specific proteins.</p> <p><b>Conclusions:</b> We found a different serum protein profile in mothers carrying DS fetuses that do not reflect expression of genes in the extra chromosome. Further studies will be necessary to establish the role of these proteins in aneuploid fetus and analyze their possible use as potential biomarkers.</p
Proteomic profile of serum of pregnant women carring a fetus with Down syndrome using nano uplc Q-tof ms/ms technology
Differences in <i>Cx</i>. <i>quinquefasciatus</i> larvae exposed to different insecticides relative to unexposed.
<p>Differences in <i>Cx</i>. <i>quinquefasciatus</i> larvae exposed to different insecticides relative to unexposed.</p
Mean and observed measurements of identified metabolites.
<p>Arg, C0, and C2 from left to right. Each dot corresponds to a sample. Crossed circle represent the mean for each treatment group. The vertical axis shows the measurements. The horizontal axis represents samples and treatment groups.</p
Heat map representing concentration of metabolites analyzed.
<p>Columns to the left show the p-value showing statistical significance in red. Insecticides (chlorpyrifos, permethrin and temephos) and the control group (larvae unexposed) are shown in first columns. Acronyms on the right are the metabolites. In the heat map, blue, white, and red, indicates low, median and high concentration, respectively.</p