12 research outputs found
Prevalence of psychopathological symptoms at rheumatological in-patients, contribution to subjective life quality assessment
The article presents the results of a cross - sectional /correlational study on the assessment of the prevalence of psychopathological symptoms in rheumatologic in-patients and their associations with quality of life.Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΊΡΠΎΡΡ β ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΈ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΎΡΠ΅Π½ΠΊΠ΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΡΠΈΡ
ΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΈΠΌΠΏΡΠΎΠΌΠ°ΡΠΈΠΊΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΡΠ΅Π²ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠ΅ΠΉ ΠΈ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΉ ΠΏΡΠΈΡ
ΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΈΠΌΠΏΡΠΎΠΌΠΎΠ² Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎΠΌ ΠΆΠΈΠ·Π½ΠΈ
Evaluation of probiotic lactobacillus as adjuvants for nasal immunization with chimeric pneumococcal vaccine
Vaccine protection against photogenic gram-positive bacteria including different species ofstreptococci is an important problem of contemporary molecular biology. Streptococcal infections are mostcommon bacterial infections surpassing by the economic losses all the infections excluding influenza. The gatesof streptococcal infection, oral cavity or vagina, are covered with immune and non-immune mucosal cells thatare the first line of defenses. Subcutaneous immunization not always stimulate the local immunity on mucosalsurfaces. On the other hand, mucosal vaccination can provide an appropriate local immune response togetherwith systemic protection. However, mucosal immunization often requires usage of special and effectiveadjuvants especially in case of vaccines based on recombinant proteins.For protection against Streptococcus pneumoniae infection, two chimeric recombinant proteins (PSPF andPSP) have been tested as vaccines. Recombinant proteins PSPF and PSP carry immunogenic epitopes fromthe respiratory pathogen including PspA, Spr1875 and PsaA. PSPF structure also carries a fraction of flagellin-FliC molecule in comparison with PSP, which does not have this fragment. This portion of PSPF was includedas internal adjuvant intended for the stimulation of Toll-like receptor 5.In this work, the adjuvant capacity of two probiotic strains, Lactobacillus rhamnosus CRL1505 andL. rhamnosus L32 was evaluated. It was demonstrated that both lactic acid bacteria strains were able to provideadjuvant effects by enhancing the mucosal and systemic immune responses after their co-administration withthe recombinant chimeric protein PSPF. The adjuvant effect of both Lactobacillus strains was significantlydecreased after their thermal inactivation. However, the cell walls of bacteria showed a marked adjuvant activity.An improved protection against several S. pneumoniae serotypes after mucosal immunization of infant micewith PSPF vaccine with probiotic strains or their cell walls was also demonstrated here.The recombinant chimeric protein PSPF administered with immunomodulatory probiotic strains or theirbacterial components would be a promising vaccine for immunization of humans against S. pneumoniae,particularly in children.ΠΠ΄Π½ΠΎΠΉ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΡ
Π·Π°Π΄Π°Ρ ΠΌΠ΅Π΄ΠΈΠΊΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°ΡΠΊΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π²Π°ΠΊΡΠΈΠ½Π½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΏΡΠΎΡΠΈΠ² ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ
ΡΡΡΠ΅ΠΏΡΠΎΠΊΠΎΠΊΠΊΠΎΠ² β ΡΠ°ΠΌΡΡ
ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»Ρ-Π½ΡΡ
Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Π΅ΠΉ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΠ΅ΡΠ± ΠΎΡ ΠΊΠΎΡΠΎΡΡΡ
ΡΡΡΡΠΏΠ°Π΅Ρ Π»ΠΈΡΡ ΠΏΠΎΡΠ΅ΡΡΠΌ ΠΎΡ Π³ΡΠΈΠΏΠΏΠΎΠ·Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΡ
ΠΎΠ΄Π½ΡΠΌΠΈ Π²ΠΎΡΠΎΡΠ°ΠΌΠΈ ΡΡΡΠ΅ΠΏΡΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΡΠ²Π»ΡΡΡΡΡ ΡΠ»ΠΈΠ·ΠΈΡΡΡΠ΅ ΠΎΠ±ΠΎ-Π»ΠΎΡΠΊΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΈ ΠΌΠΎΡΠ΅ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ°.
ΠΠ°ΡΠ΅Π½ΡΠ΅ΡΠ°Π»ΡΠ½ΡΠΉ ΡΠΏΠΎΡΠΎΠ± Π²Π²Π΅Π΄Π΅Π½ΠΈΡ Π²Π°ΠΊΡΠΈΠ½ Π½Π΅ Π²ΡΠ΅Π³Π΄Π° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π΄ΠΎΠ±ΠΈΠ²Π°ΡΡΡΡ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²-Π½ΠΎΠΉ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΠΈ ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½ΠΈΡΠ΅ΡΠ° Π½Π° ΡΠ»ΠΈΠ·ΠΈΡΡΡΡ
ΠΎΠ±ΠΎΠ»ΠΎΡΠΊΠ°Ρ
, Π° Π²Π°ΠΊΡΠΈΠ½Ρ, Π²Π²ΠΎΠ΄ΠΈΠΌΡΠ΅ ΡΠ΅ΡΠ΅Π· ΡΠ»ΠΈΠ·ΠΈ-ΡΡΡΠ΅ ΠΎΠ±ΠΎΠ»ΠΎΡΠΊΠΈ, ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°ΡΡ ΠΈΠΌΠΌΡΠ½Π½ΡΡ Π·Π°ΡΠΈΡΡ Π² ΠΎΠ±Π»Π°ΡΡΠΈ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ, Π° ΡΠ°ΠΊΠΆΠ΅
ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΡΠΈΡΡΠ΅ΠΌΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ°.
ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ ΡΠ΅ΡΠ΅Π· ΡΠ»ΠΈΠ·ΠΈΡΡΡΠ΅ ΠΎΠ±ΠΎΠ»ΠΎΡΠΊΠΈ Π²Π°ΠΊΡΠΈΠ½Π½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ ΡΡΠ΅Π±ΡΠ΅Ρ ΠΈΡΠΏΠΎΠ»Ρ-Π·ΠΎΠ²Π°Π½ΠΈΡ ΡΠΏΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΠΈ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΡΡ
Π°Π΄ΡΡΠ²Π°Π½ΡΠΎΠ², ΠΏΠΎΡΠΊΠΎΠ»ΡΠΊΡ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ ΠΎΠ±ΡΡΠ½ΠΎ ΠΏΡΠΎΡΠ²Π»ΡΡΡ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ ΠΌΠΌΡΠ½ΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΏΡΠΈ ΡΠ°ΠΊΠΎΠΌ ΡΠΏΠΎΡΠΎΠ±Π΅ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ. Π ΡΠ°Π±ΠΎΡΠ΅ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅
Π²Π°ΠΊΡΠΈΠ½Π½ΡΡ
Π°Π΄ΡΡΠ²Π°Π½ΡΠΎΠ² ΠΏΡΠΈ ΠΌΡΠΊΠΎΠ·Π°Π»ΡΠ½ΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΈΠ·Π°ΡΠΈΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΏΠ½Π΅Π²ΠΌΠΎΠΊΠΎΠΊΠΊΠΎΠ²ΡΠΌΠΈ Ρ
ΠΈΠΌΠ΅ΡΠ½ΡΠΌΠΈ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΌΠΈ Π±Π΅Π»ΠΊΠ°ΠΌΠΈ PSPF ΠΈ PSP Π±ΡΠ»ΠΈ Π°ΠΏΡΠΎΠ±ΠΈΡΠΎΠ²Π°Π½Ρ Π΄Π²Π° ΡΡΠ°ΠΌΠΌΠ° ΠΏΡΠΎΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ² β Lactobacillus rhamnosus CRL1505 ΠΈ L32. Π Π΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠ΅ Ρ
ΠΈΠΌΠ΅ΡΠ½ΡΠ΅ Π±Π΅Π»ΠΊΠΈ PSPF ΠΈ PSP Π½Π΅ΡΡΡ Π² ΡΠ²ΠΎΠ΅ΠΉ ΡΡΡΡΠΊΡΡΡΠ΅ Π½Π΅ΡΠΊΠΎΠ»ΡΠΊΠΎ ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π΅Π½Π½ΡΡ
ΡΠΏΠΈΡΠΎΠΏΠΎΠ² PspA, Spr1875, PsaA ΠΈ ΠΏΡΠ΅Π΄Π½Π°Π·Π½Π°ΡΠ΅Π½Ρ Π΄Π»Ρ Π²Π°ΠΊΡΠΈΠ½Π°ΡΠΈΠΈ ΠΏΡΠΎΡΠΈΠ² ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Streptococcus pneumoniae. ΠΠ΅Π»ΠΊΠΈ, ΠΎΡΠ»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ
ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π² ΡΡΡΡΠΊ-ΡΡΡΠ΅ PSPF ΡΡΠ°ΡΡΠΊΠ° ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΡΠ»Π°Π³Π΅Π»Π»ΠΈΠ½Π° β FliC, ΠΏΠΎ-ΡΠ°Π·Π½ΠΎΠΌΡ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈΠΌΠΌΡΠ½Π½ΡΠΉ ΠΎΡΠ²Π΅Ρ ΠΏΡΠΈ
ΡΠΎΠ²ΠΌΠ΅ΡΡΠ½ΠΎΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ Ρ Π΄Π²ΡΠΌΡ ΡΡΠ°ΠΌΠΌΠ°ΠΌΠΈ ΠΏΡΠΎΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ². Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΎΠ±Π° ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
ΡΡΠ°ΠΌ-ΠΌΠ° L. rhamnosus Π±ΡΠ»ΠΈ ΡΠΏΠΎΡΠΎΠ±Π½Ρ ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π°Π΄ΡΡΠ²Π°Π½ΡΠ½ΡΠΉ ΡΡΡΠ΅ΠΊΡ ΠΏΡΠΈ ΠΈΠ½ΡΡΠ°Π½Π°Π·Π°Π»ΡΠ½ΠΎΠΌ Π²Π²Π΅Π΄Π΅Π½ΠΈΠΈ Π²Π°ΠΊ-ΡΠΈΠ½Π½ΡΡ
Π±Π΅Π»ΠΊΠΎΠ², ΠΏΡΠΎΡΠ²Π»ΡΠ²ΡΠΈΠΉΡΡ Π² ΡΡΠΈΠ»Π΅Π½ΠΈΠΈ ΡΠ΅ΠΊΡΠ΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΈ Π³ΡΠΌΠΎΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° Π½Π° ΡΠΎ-Π²ΠΌΠ΅ΡΡΠ½ΠΎ Π²Π²Π΅Π΄Π΅Π½Π½ΡΠΉ ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΠΉ Ρ
ΠΈΠΌΠ΅ΡΠ½ΡΠΉ Π±Π΅Π»ΠΎΠΊ PSPF. ΠΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
IgA Π½ΠΎΡΠΎΠ²ΡΡ
ΡΠΌΡΠ²ΠΎΠ² ΠΈ IgG ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ Π½Π° PSP ΠΏΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ L. rhamnosus L32 Π½Π΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΠ»ΠΎ. ΠΠ΄ΡΡΠ²Π°Π½ΡΠ½ΡΠΉ ΡΡΡΠ΅ΠΊΡ ΠΎΡ Π²Π²ΠΎΠ΄ΠΈΠΌΡΡ
Π»Π°ΠΊΡΠΎΠ±Π°ΡΠΈΠ»Π»ΡΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ½ΠΈ-ΠΆΠ°Π»ΡΡ ΠΏΠΎΡΠ»Π΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ½ΠΎΠΉ ΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΡΠ΅Π½ΠΎΠΊ L. rhamnosus CRL1505 ΠΏΡΠΎΡΠ²Π»ΡΠ» Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ. Π‘ΡΠΈΠΌΡΠ»ΡΡΠΈΡ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° Π°Π΄ΡΡΠ²Π°Π½ΡΠ°ΠΌΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΊ ΡΡΠΈΠ»Π΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ° Π²Π°ΠΊΡΠΈΠ½Ρ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Ρ
Π½Π° Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
, ΠΈΠ½ΡΠΈΡΠΈ-ΡΠΎΠ²Π°Π½Π½ΡΡ
S. pneumoniae. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΡΡΠ°ΠΌΠΌΡ Π»Π°ΠΊΡΠΎΠ±Π°ΡΠΈΠ»Π», Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ Lactobacillus rhamnosus CRL1505 ΠΈ L32, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π°Π΄ΡΡΠ²Π°Π½ΡΠΎΠ² Π² ΡΠΎΡΡΠ°Π²Π΅ ΠΌΡΠΊΠΎΠ·Π°Π»ΡΠ½ΡΡ
Π²Π°ΠΊΡΠΈΠ½, ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΡΠ° ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΡΠ²ΠΎΠΉΡΡΠ² Π²Π°ΠΊΡΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΈ ΡΠΎΡΠΌΡ Π²Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΡΠΎΠ±ΠΈΠΎΡΠΈΠΊΠΎΠ².Fil: Leontieva, G. F.. Institute of Experimental Medicine; RusiaFil: Kramskaya, T. A.. Institute of Experimental Medicine; RusiaFil: Grabovskaya, K. B.. Institute of Experimental Medicine; Rusia; RusiaFil: Filimonova, V. Yu.. Institute of Experimental Medicine; Rusia; RusiaFil: LaiΓ±o, Jonathan Emiliano. Consejo Nacional de Investigaciones CientΓficas y TΓ©cnicas. Centro CientΓfico TecnolΓ³gico Conicet - TucumΓ‘n. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones CientΓficas y TΓ©cnicas. Centro CientΓfico TecnolΓ³gico Conicet - TucumΓ‘n. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones CientΓficas y TΓ©cnicas. Centro CientΓfico TecnolΓ³gico Conicet - TucumΓ‘n. Centro de Referencia para Lactobacilos; ArgentinaFil: Danilenko, V. N.. Academy of Sciences. Institute of General Genetics. Head, Division of Fundamental Genetic Studies in Biotechnology; RusiaFil: Suvorov, A. N.. St. Peterburg State University; Rusia. Institute of Experimental Medicine. Head, Division of Molecular Microbiology; Rusi
ANTIMICROBIAL AND IMMUNOMODULATING ACTIVITY OF A TOPICAL GEL CONTAINING ACTIVE PEPTIDE COMPONENTS ON THE MODEL OF EXPERIMENTAL BACTERIAL VAGINITIS
EFFECTS OF DIFFERENT ADJUVANTS UPON IMMUNOGENICITY OF ANTI-GROUP B STREPTOCOCCAL VACCINE COMPONENTS
Abstract. Design of an effective and safe vaccine against pathogenic streptococci is still on the agenda, in spite of numerous attempts in this area undertaken by different laboratories. In order to improve immunogenicity of recombinant vaccine preparations, a selection of effective adjuvants is necessary. Previously, two recombinant GBS polypeptides P6 and ScaAB were found to be immunogenic, and their injection in separate preparations or mixed manner boosted production of specific and protective antibodies with high affinity. Four different adjuvants (Freund adjuvant, aluminum hydroxide, Bestim and Interleukine-1Ξ²) have been tested for immunization of mice with single polypeptides, or with their mixtures. As a result of vaccination, it was demonstrated that aluminum hydroxide was providing the most desirable immunological parameters of immune response among the adjuvants tested. A mixture of polypeptides containing aluminum hydroxide was found to produce specific antibodies with better opsonizing activity against group B streptococci. (Med. Immunol., 2008, vol. 10, N 2-3, pp 215-222)