Kaposi’s Sarcoma-Associated Herpesvirus microRNAs

Kaposi’s sarcoma-associated herpesvirus (KSHV) is a human pathogenic γ-herpesvirus strongly associated with the development of Kaposi’s Sarcoma and B cell proliferative disorders, including primary effusion lymphoma (PEL). The identification and functional investigation of non-coding RNAs expressed by KSHV is a topic with rapidly emerging importance. KSHV miRNAs derived from 12 stem-loops located in the major latency locus have been the focus of particular attention. Recent studies describing the transcriptome-wide identification of mRNA targets of the KSHV miRNAs suggest that these miRNAs have evolved a highly complex network of interactions with the cellular and viral transcriptomes. Relatively few KSHV miRNA targets, however, have been characterized at a functional level. Here, our current understanding of KSHV miRNA expression, targets, and function will be reviewed

River monitoring from satellite radar altimetry in the Zambezi River basin

Satellite radar altimetry can be used to monitor surface water levels from space. While current and past altimetry missions were designed to study oceans, retracking the waveforms returned over land allows data to be retrieved for smaller water bodies or narrow rivers. The objective of this study is the assessment of the potential for river monitoring from radar altimetry in terms of water level and discharge in the Zambezi River basin. Retracked Envisat altimetry data were extracted over the Zambezi River basin using a detailed river mask based on Landsat imagery. This allowed for stage measurements to be obtained for rivers down to 80m wide with an RMSE relative to in situ levels of 0.32 to 0.72m at different locations. The altimetric levels were then converted to discharge using three different methods adapted to different data-availability scenarios: first with an in situ rating curve available, secondly with one simultaneous field measurement of cross-section and discharge, and finally with only historical discharge data available. For the two locations at which all three methods could be applied, the accuracies of the different methods were found to be comparable, with RMSE values ranging from 4.1 to 6.5% of the mean annual in situ gauged amplitude for the first method and from 6.9 to 13.8% for the second and third methods. The precision obtained with the different methods was analyzed by running Monte Carlo simulations and also showed comparable values for the three approaches with standard deviations found between 5.7 and 7.2% of the mean annual in situ gauged amplitude for the first method and from 8.7 to 13.0% for the second and third methods

Operational reservoir inflow forecasting with radar altimetry: The Zambezi case study

River basin management can greatly benefit from short-term river discharge predictions. In order to improve model produced discharge forecasts, data assimilation allows for the integration of current observations of the hydrological system to produce improved forecasts and reduce prediction uncertainty. Data assimilation is widely used in operational applications to update hydrological models with in situ discharge or level measurements. In areas where timely access to in situ data is not possible, remote sensing data products can be used in assimilation schemes. <br><br> While river discharge itself cannot be measured from space, radar altimetry can track surface water level variations at crossing locations between the satellite ground track and the river system called virtual stations (VS). Use of radar altimetry versus traditional monitoring in operational settings is complicated by the low temporal resolution of the data (between 10 and 35 days revisit time at a VS depending on the satellite) as well as the fact that the location of the measurements is not necessarily at the point of interest. However, combining radar altimetry from multiple VS with hydrological models can help overcome these limitations. <br><br> In this study, a rainfall runoff model of the Zambezi River basin is built using remote sensing data sets and used to drive a routing scheme coupled to a simple floodplain model. The extended Kalman filter is used to update the states in the routing model with data from 9 Envisat VS. Model fit was improved through assimilation with the Nash–Sutcliffe model efficiencies increasing from 0.19 to 0.62 and from 0.82 to 0.88 at the outlets of two distinct watersheds, the initial NSE (Nash–Sutcliffe efficiency) being low at one outlet due to large errors in the precipitation data set. However, model reliability was poor in one watershed with only 58 and 44% of observations falling in the 90% confidence bounds, for the open loop and assimilation runs respectively, pointing to problems with the simple approach used to represent model error

Multi-Objective Optimization for Analysis of Changing Trade-Offs in the Nepalese Water-Energy-Food Nexus with Hydropower Development

While the water–energy–food nexus approach is becoming increasingly important for more efficient resource utilization and economic development, limited quantitative tools are available to incorporate the approach in decision-making. We propose a spatially explicit framework that couples two well-established water and power system models to develop a decision support tool combining multiple nexus objectives in a linear objective function. To demonstrate our framework, we compare eight Nepalese power development scenarios based on five nexus objectives: minimization of power deficit, maintenance of water availability for irrigation to support food self-sufficiency, reduction in flood risk, maintenance of environmental flows, and maximization of power export. The deterministic multi-objective optimization model is spatially resolved to enable realistic representation of the nexus linkages and accounts for power transmission constraints using an optimal power flow approach. Basin inflows, hydropower plant specifications, reservoir characteristics, reservoir rules, irrigation water demand, environmental flow requirements, power demand, and transmission line properties are provided as model inputs. The trade-offs and synergies among these objectives were visualized for each scenario under multiple environmental flow and power demand requirements. Spatially disaggregated model outputs allowed for the comparison of scenarios not only based on fulfillment of nexus objectives but also scenario compatibility with existing infrastructure, supporting the identification of projects that enhance overall system efficiency. Though the model is applied to the Nepalese nexus from a power development perspective here, it can be extended and adapted for other problems

Combining satellite radar altimetry, SAR surface soil moisture and GRACE total storage changes for hydrological model calibration in a large poorly gauged catchment

The availability of data is a major challenge for hydrological modelling in large parts of the world. Remote sensing data can be exploited to improve models of ungauged or poorly gauged catchments. In this study we combine three datasets for calibration of a rainfall-runoff model of the poorly gauged Okavango catchment in Southern Africa: (i) surface soil moisture (SSM) estimates derived from radar measurements onboard the Envisat satellite; (ii) radar altimetry measurements by Envisat providing river stages in the tributaries of the Okavango catchment, down to a minimum river width of about one hundred meters; and (iii) temporal changes of the Earth's gravity field recorded by the Gravity Recovery and Climate Experiment (GRACE) caused by total water storage changes in the catchment. The SSM data are shown to be helpful in identifying periods with over-respectively underestimation of the precipitation input. The accuracy of the radar altimetry data is validated on gauged subbasins of the catchment and altimetry data of an ungauged subbasin is used for model calibration. The radar altimetry data are important to condition model parameters related to channel morphology such as Manning's roughness. GRACE data are used to validate the model and to condition model parameters related to various storage compartments in the hydrological model (e.g. soil, groundwater, bank storage etc.). As precipitation input the FEWS-Net RFE, TRMM 3B42 and ECMWF ERA-Interim datasets are considered and compared