3D-Plotting Algorithm for Insects using YOLOv5

In ecological research, accurately collecting spatiotemporal position data is a fundamental task for understanding the behavior and ecology of insects and other organisms. In recent years, advancements in computer vision techniques have reached a stage of maturity where they can support, and in some cases, replace manual observation. In this study, a simple and inexpensive method for monitoring insects in three dimensions (3D) was developed so that their behavior could be observed automatically in experimental environments. The main achievements of this study have been to create a 3D monitoring algorithm using inexpensive cameras and other equipment to design an adjusting algorithm for depth error, and to validate how our plotting algorithm is quantitatively precise, all of which had not been realized in conventional studies. By offering detailed 3D visualizations of insects, the plotting algorithm aids researchers in more effectively comprehending how insects interact within their environments

Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi

Melanocytic nevi are thought to be senescent clones of melanocytes that have acquired an oncogenic BRAF mutation. BRAF mutation is considered to be a crucial step in the initiation of melanocyte transformation. However, using immunomagnetic separation or laser-capture microdissection, we examined BRAF mutations in sets of approximately 50 single cells isolated from acquired melanocytic nevi from 13 patients and found a substantial number of nevus cells that contained wild-type BRAF mixed with nevus cells that contained BRAF(V600E). Furthermore, we simultaneously amplified BRAF exon 15 and a neighboring single nucleotide polymorphism (SNP), rs7801086, from nevus cell samples obtained from four patients who were heterozygous for this SNP. Subcloning and sequencing of the polymerase chain reaction products showed that both SNP alleles harbored the BRAF(V600E) mutation, indicating that the same BRAF(V600E) mutation originated from different cells. The polyclonality of BRAF mutations in acquired melanocytic nevi suggests that mutation of BRAF may not be an initial event in melanocyte transformation.ArticleJOURNAL OF THE NATIONAL CANCER INSTITUTE. 101(20):1423-1427 (2009)journal articl

Paleoenvironmental significance of growth story of long-living deep-water acervulinid macroids from Kikai-jima shelf, Central Ryukyu Islands, Japan

Macroids are unattached centimetre-sized nodules built by encrusting invertebrates. Encrusting foraminifera (Acervulina inhaerens) and subordinate thin coralline algae form extensive macroid beds on sandy and gravelly bioclastic carbonates off Kikai-jima, on a coral-reef-related island shelf, in the Central Ryukyu Islands, Japan. At water depths from 75 to 100 m, the beds consist of spheroidal and sub-spheroidal macroids, c. 6 cm in mean diameter, with asymmetric concentric inner arrangement. The macroids are bioeroded by Entobia, Maeandropolydora, Trypanites, Gastrochaenolites, and microborings. They generally show two distinct growth stages separated by an abraded rugged surface deeply colonized by borers, mainly Entobia. Radiocarbon dating yielded an oldest age of c. 4400 cal yr BP for the earliest acervulinid growth, whereas the second stages were much younger, ranging in age from c. 1500 cal yr BP to present day. Datings of the two growth stages in five specimens indicate that active growth and growth interruption were not synchronous in the different nodules. For c. 4400 years the macroids grew within an estimated maximum range of palaeotemperature changes of c. 4.7 ◦C, under chronic oligotrophic to mesotrophic conditions, low-level hydrodynamism and low sedimentation rates. The lack of synchroneity among individual macroids rules out catastrophic events and ecosystem-wide environmental changes as possible causes of growth interruption. Random biogenic mobilization and temporal occupation of the macroid surface by organisms with no rigid skeleton and/or biofilms likely interrupted acervulinid growth at individual macroid scale. The environmental conditions in which Kikai-jima macroid beds develop do not support interpretations of acervulinid macroid accumulations during Paleocene-Eocene Thermal Maximum (PETM) and Middle Eocene Climatic Optimum (MECO) events in the Western Tethys as indicators of eutrophic conditions

Cancer Cells Expressing Toll-like Receptors and the Tumor Microenvironment

Toll-like receptors (TLRs) play a crucial role in the innate immune response and the subsequent induction of adaptive immune responses against microbial infection or tissue injury. Recent findings show that functional TLRs are expressed not only on immune cells but also on cancer cells. TLRs play an active role in carcinogenesis and tumor progression during chronic inflammation that involves the tumor microenvironment. Damage-associated molecular patterns (DAMPs) derived from injured normal epithelial cells and necrotic cancer cells appear to be present at significant levels in the tumor microenvironment, and their stimulation of specific TLRs can foster chronic inflammation. This review discusses how carcinogenesis, cancer progression, and site-specific metastasis are related to interactions between cancer cells, immune cells, and DAMPs through TLR activation in the tumor microenvironment

イデンセイ ジン シッカン マウス ニ オケル ジン センイショウ ハッショウ キコウ ニ カンスル ケンキュウ

京都大学0048新制・課程博士博士(農学)甲第12356号農博第1537号新制||農||923(附属図書館)学位論文||H18||N4114(農学部図書室)24192UT51-2006-J348京都大学大学院農学研究科応用生物科学専攻(主査)教授 久米 新一, 教授 矢野 秀雄, 教授 今井 裕学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA