Iowa Swiss-type cheese

New types of cheese for Iowa have been receiving the attention of the Iowa Agricultural Experiment Station for a number of years. A previous publication (1) described the method of manufacture which has been used in the production of many thousands of pounds of Iowa Blue Cheese. This publication deals with the process used in the Iowa State College laboratories in manufacturing a Swiss-type cheese. In the course of these experiments a total of 25,136 lbs. of the cheese has been manufactured and marketed, utilizing approximately a quarter of a million pounds of milk

Iowa blue cheese

Iowa is an importer of cheese. In 1933 Iowa dairy plants manufactured 1,491,822 pounds of cheese.2 In the same year consumption is estimated to have been 10,254,397 pounds, using the 1933 United States Department of Agriculture figure of 4.15 pounds per capita and 1930 Iowa census figures as a basis of computation. In 1933 Iowa dairy plants produced 14.6 percent of the cheese consumed in the state. If this percentage could be greatly increased it would result in a larger and more diversified market for Iowa milk. Production of cured cheese in Iowa has up to the present consisted almost entirely of the staple variety known as Cheddar or American cheese. Small production has not been the result either of lack of milk or of inability to produce an acceptable cheese. Rather it has been the inability of the average dairy plant to pay enough more for milk to be used for cheesemaking to divert the milk from other manufacturing uses, principally butter. The high value placed by the Iowa farmer upon skimmilk for feeding purposes when used as a supplement to corn in hog production has undoubtedly been one important factor in limiting the production of cheese. When milk is made into cheese the skimmilk is not available for feeding on the farm. Instead, whey, which is estimated to possess half the value of skimmilk, is available for the feeding operations. This and other factors require that the dairy plants must be able to pay a substantially higher price for milk fat for cheesemaking than for buttermaking if milk is to be available for the former. Expansion of cheese production in Iowa apparently depends upon some method of increasing the returns which can be obtained from cheese so that a relatively larger payment can be made to the milk producer

Inhibition of Pokeweed Antiviral Protein (PAP) by turnip mosaic virus genome-linked protein (VPg)

Pokeweed antiviral protein (PAP) from Phytolacca americana is a ribosome-inactivating protein (RIP) and an RNA N-glycosidase that removes specific purine residues from the sarcin/ricin loop of large rRNA, arresting protein synthesis at the translocation step. PAP is also a cap-binding protein and is a potent antiviral agent against many plant, animal, and human viruses. To elucidate the mechanism of RNA depurination, and to understand how PAP recognizes and targets various RNAs, the interactions between PAP and turnip mosaic virus genomelinked protein (VPg) were investigated. VPg can function as a cap analog in cap-independent translation and potentially target PAP to uncapped IRES-containing RNA. In this work, fluorescence spectroscopy andHPLCtechniques were used to quantitatively describe PAP depurination activity and PAP-VPg interactions. PAP binds to VPg with high affinity (29.5 nM); the reaction is enthalpically driven and entropically favored. Further, VPg is a potent inhibitor of PAP depurination of RNA in wheat germ lysate and competes with structured RNA derived from tobacco etch virus for PAP binding. VPg may confer an evolutionary advantage by suppressing one of the plant defense mechanisms and also suggests the possible use of this protein against the cytotoxic activity of ribosome-inactivating proteins. Background: PAP is a ribosome-inactivating protein that depurinates RNA and inhibits protein synthesis. Results: Turnip mosaic VPg inhibits enzymatic activity of PAP in wheat germ extract. Conclusion: VPg may play a role in overcoming viral resistance by suppressing the plant defense mechanism. Significance: Depurination inhibition by VPg suggests a novel viral strategy to evade host cell defense and possible anticytotoxic activity against RIPs

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

We have examined the developmental origins of Ng2+ perivascular cell populations that adhere to the basement membrane of blood vessels, and their contribution to wound healing. Neural/glial antigen 2 (Ng2) labeled most perivascular cells (70-80%) in developing and adult mouse back skin, a higher proportion than expressed by other pericyte markers Tbx18, Nestin and Pdgfrβ. In adult mouse back skin Ng2+ perivascular cells could be categorized into 4 populations based on whether they expressed Pdgfrα and Pdgfrβ individually or in combination or were Pdgfr-negative. Lineage tracing demonstrated that although Ng2+ cells in embryonic and neonatal back skin contributed to multiple cell types they did not give rise to interfollicular fibroblasts within the dermis. Lineage tracing of distinct fibroblast populations during skin development showed that papillary fibroblasts (Lrig1+) gave rise to Ng2+ perivascular cells in the upper dermis, whilst Ng2+ perivascular cells in the lower dermis were primarily derived from reticular Dlk1+ fibroblasts. Following wounding of adult skin, Ng2+ dermal cells only give rise to Ng2+ blood vessel associated cells and did not contribute to other fibroblast lineages. The relative abundance of Ng2+ Pdgfrβ+ perivascular populations was comparable in wounded and non-wounded skin, indicating that perivascular heterogeneity was maintained during full thickness skin repair. In the wound bed Ng2+ perivascular populations were primarily derived from Lrig1+ papillary or Dlk1+ reticular fibroblast lineages, according to the location of the regenerating blood vessels. We conclude that Ng2+ perivascular cells represent a heterogeneous lineage restricted population that is primarily recruited from the papillary or reticular fibroblast lineages during tissue regeneration