Effect of acoustic pulses and EHF radiation on multipotent marrow stromal cells in tissue engineering constructs

In this paper, we studied the effects of physical factors, such as, acoustic pulses of laser-induced hydrodynamics (ALIH) and extremely-high frequencies (EHF) radiation, on the formation of heterotopic bone marrow organs. A suspension of precipitated bone marrow cells from CBA mice were exposed to ALIH pulses and EHF radiation separately and in their combination tissue engineering constructs, presenting gelatin sponges 2 by 2 by 2mm in size containing 107 nucleated bone marrow cells, were exposed to physical factors and were implanted under the renal capsules of syngeneic mice. The newly formed hematopoietic organs were examined in three and five months later after treatment. The five months old transplants were bigger in size than the three months old transplants. The number of hematopoietic cells in the rest of the groups increased during this period by a factor from 3 to 10, the increase being as high as 20-fold in the ALIH+EHF group. Maximal concentration of multipotent stromal cells (MSCs) was in the EHF+ALIH, and minimal concentration was in the ALIH+EHF. The accumulation rate of bone capsule weight was highest for the transplants of EHF+ALIH and ALIH-sponge groups during the first three months. These data showed that the combined impact of the EHF+ALIH on MSCs is the most effective for the formation of bone marrow transplantation