182 research outputs found
Antioxidant capacity and toxicological evaluation of pterospartum tridentatum flower extracts
Pterospartum tridentatum Willk. (prickled broom) is an autochthonous plant, common
in Portuguese territory.
The yellow flowers are used in traditional medicine, as a potential cure for all body
illnesses, mainly for throat irritation treatment or for diabetes, hypertension and
hypercholesterolemia therapy.Despite its traditional use, no toxicological assessment has been performed as we know.
A high antioxidant activity of P. tridentatum flower water extract was acessed in good
agreement with its ESI-MS spectrum that revealed the presence of several flavonoids, as
luteolin-O-(O-acetyl)-glucuronide, luteolin-O- glucuronide or isorhamnetin-O-hexoside.
Mitocondrial respiratory rates (state 4, state 3 and FCCP-stimulated respiration) and
respiratory indexes (respiratory control and P/O ratios) showed no consistent decrease
of respiratory and phosphorylative efficiencies for the concentrations tested (up to 500
ÎŒg.mL-1). Cytotoxicity evaluation, using MTT assay, was reliable with the previous
results. In conclusion, for the concentration range commonly used P. tridentatum
flowers usage can be regarded as harmless and trustworthy
Thyroid control over biomembranes: VI. Lipids in liver mitochondria and microsomes of hypothyroid rats
The lipids of liver mitochondria prepared from normal rats and from rats made hypothyroid by thyroidectomy and injection with131INa contained similar amounts, per mg protein, of total lipids, phospholipids, neutral lipids and lipid phosphorus. Hypothyroidism caused a doubling of the relative amounts of mitochondrial cardiolipins (CL; to 20.5% of the phospholipid P) and an accompanying trend (although statistically not significant) toward decreased amounts of both phosphatidylcholines (PC) and phosphatidylserines (PS), with phosphatidylethanolamines (PE) remaining unchanged. The pattern of elevated 18â¶2 fatty acyl content and depleted 20â¶4 acyl groups of the mitochondrial phospholipids of hypothyroid preparations was reflected to varying degrees in the resolved phospholipids, with PC showing greater degrees of abnormality than PE, and CL showing none. Hypothyroidism produced the same abnormal pattern of fatty acyl distributions in liver microsomal total lipids as was found in the mitochondria. Hypothyroid rats, when killed 6 hr after injection of [1â14C] labeled linoleate, showed the following abnormalities: the liver incorporated less label into lipids, and converted 18â¶2 not exclusively to 20â¶4 (as normals do) but instead incorporated the label mainly into saturated fatty acids. These data, together with the known decrease in ÎČâoxidation, suggest that hypothyroidism involves possible defective step(s) in the conversion of 18â¶2 to 20â¶4.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142296/1/lipd0328.pd
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