PERK Pathway Dataset.

<p>PERK Pathway Dataset.</p

IRE-1 Pathway.

<p>IRE-1 Pathway.</p

Clinical characteristics of 7 surgical patients.

<p>Clinical characteristics of 7 surgical patients.</p

ATF-6 Pathway Dataset.

<p>ATF-6 Pathway Dataset.</p

Genotyping of founder βIKK (A) and βISR (B) mice.

<p>Genotyping of founder βIKK (A) and βISR (B) mice.</p

Translocation of NF-κB to the nucleus in islet cells from βIKK and βISR 8–12 week old mice in the presence or absence of 10 U/ml IL-1, using immunohistochemical detection of the NF-κB subunit p50, by an antibody specific for nuclear localized p50.

<p>Translocation of NF-κB to the nucleus in islet cells from βIKK and βISR 8–12 week old mice in the presence or absence of 10 U/ml IL-1, using immunohistochemical detection of the NF-κB subunit p50, by an antibody specific for nuclear localized p50.</p

Constructs used to create βIKK and βISR mice.

<p>Dominant active IKKβ (S177/181E) or a non-degradable form of IkBα (S32/36A; super repressor) were expressed selectively in beta cells using the rat insulin 2 promoter to produce the βIKK and βISR mice, respectively. N-terminal FLAG or His tag sequences were included in exon 2 of a β-globin splicing cassette</p

NF-κB localization in βIKK and wild-type islets 98 days after transplantation.

<p>In wild-type islets (panel A and C), the NF-κB localization is cytoplasmic whereas in βIKK islets (panel B) the localization is nuclear. Scale bar is 20 µm.</p

Blood glucose levels after transplantation of 150 islets from βISR mice (closed circles) or βIKK mice (closed triangles) and their respective wild-type littermates (open symbols) into STZ diabetic male C57BL/6AF1 recipients.

<p>n = 8–13. * = p<0.05, t-test vs wild-type littermates of βIKK mice, n = 12–13.</p

Insulin secretion from wild type (WT) and βIKK islets after culture for 48 h in 2.5 U/ml IL-1β, 33 mmol/l glucose or in control media.

<p>n = 5–7 separate experiments with triplicates.</p