Effect of inorganic chelate of zinc and restaurant residual oil added to feed mixture on the biochemical traits of thigh muscles in male broilers

An experiment was planned to study the influence of restaurant residual oil (RRO) and inorganic chelate of Zn (ZnO) on triglyceride (TRG), cholesterol (CHOL) and malondialdehyde (MDA) concentrations of thigh muscles in male broiler chickens. In the present research, three hundred and twenty four 10-day-old male broiler chicks (Ross 308 strain) in nine treatments including three levels of experimental oil (0, 2.5 and 5%) and three levels of ZnO (0, 50 and 100 mg/kg of feed) were fed until 42 days. The results showed that using RRO, total biochemical traits (TRG, CHOL and MDA) of muscles increased, MDA (p < 0.01), CHOL (p <0.01) and TRG (p < 0.05). Also, different levels of zinc oxide supplement significantly decreased the content of MDA and CHOL in thigh (p < 0.05), but did not result to a significant alteration in TRG concentration. The interaction effects of RRO and ZnO did not result to a significant change in total biochemical traits of fresh thigh muscles in male broilers. Therefore, the effects of RRO deteriorated the quality of meat by raising the susceptibility of muscles to free radical oxidative damage. Also, the effects of ZnO supplementation improved the quality of meat by reducing the extent of oxidation of muscles.Key words: Residual oil, zinc, biochemical trait, muscle, broile

Isolation of nocardia from bronchoalveolar lavage and sputum specimens of patients referring to Isfahan T.B center, 2005.

Background: Pulmonary nocardiosis is a dangerous chronic infection caused by implantation of aerobic actinomycetes called nocardia in respiratory system. The disease is more commonly found in patients with chronic infectious disorders, which may be treated by prolonged courses of antibiotics or corticosteroids, and in immunosuppressed patients. While pulmonary and systemic nocardiosis has no pathognomonic clinical or radiographic features it has the same clinical symptoms as tuberculosis, with a treatment much more different. The aim of this study was evaluation of nocardiosis among suspected pulmonary patients being confined or referred to Isfahan T.B center, in 2005. Materials and Methods: In this descriptive study 200 reliable samples, including 90 BAL (Bronchoalveolar lavage) and 110 sputum specimens, were collected during a period of 12 months from patients with diverse bronchopulmonary diseases, whether confined or referred to the center. 53.5 of patients were male and the rest were female. The data were statistically analyzed by SPSS Software using Fisher Exact Test. Results: The results obtained through direct smears (KOH, BM, Kynion), culture and diagnostically - identical methods revealed eight positive cases (4) of Nocardia Asteroids. Of 107 males, 6 (5.6) and of 93 females, two (2.2) were infected by Nocardia. There was no significant difference concerning age, sex, occupation, and nocardiosis. Conclusion: Because of high incidence of nocardiosis found in this study, paying attention to nocardiosis in patients suffering from pulmonary diseases is highly recommended. Nocardiosis can become a severe infection which profoundly affects immunocompromised patients. Differential diagnosis often delays the time of diagnosis, which worsens the out come

A MspI PCR-RFLP within bovin growth hormone gene and its association with sperm quality traits in Iranian Holstein bulls

The present study was aimed to examine the association of bovine growth hormone gene polymorphism with sperm quality traits including sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility(PTSM), total post thaw motile sperm (TPTMS), total sperm dose (TSD) and testis biometry trait as average testis length (ATL), average testis width (ATW) and scrotum circumference (SC) in Iranian Holstein bulls. PCR-RFLP method with Msp-I restriction enzyme was used for genotyping. The frequency of the MspI+(C) and MspI-(D) alleles are 0.883 and 0.117, respectively. The genotype frequency for CC, CD and DD were 0.787, 0.191 and 0.022, respectively. The DD genotype was omitted of analysis. Mixed model analyses of sperm quality traits considering genotype and environment as fixed effects and animal as a random effect suggested that sire was a significant source of variation (P< 0.001) in all traits. The CC genotype resulted in a significant increase in SV (p = 0.022), FSM (

Detection of proopiomelanocortin-derived antigens in normal and pathologic human skin

We investigated the presence of proopiomelanocortin (POMC) products in sections of skin from normal subjects and patients with neoplastic and non-neoplastic cutaneous disorders. Antibodies specific against adrenocorticotropin, beta-melanotropin, and beta-endorphin were used for detection and characterization of cell types bearing POMC peptides. POMC products were not observed in sections of normal skin from the corporal (non-scalp) areas (six cases), whereas the hair follicles of scalp skin exhibited positive immunostains that were readily apparent (four cases). POMC products were frequently detected in corporal skin affected by diseases (13 of 26 cases), for example, psoriatic keratinocytes, the inflammatory infiltrate in scarring alopecia, nevocytes, the epithelial cell nests of basal cell carcinoma, and melanoma cells. Further tests were performed in keloids, a primary reactive skin disorder, to evaluate whether POMC accumulation represented a disease-related phenomenon or an expression of normal cutaneous reactivity. POMC products were consistently detected (10 of 11 cases) in the keratinocytes and mononuclear cells at keloid lesions. Thus these observations indicate that POMC products may accumulate locally in lesional skin representing, presumably, a novel cutaneous response to injury. The broad spectrum of POMC products detected suggests that these arise from production in situ (expression of the POMC gene itself) by human skin

Flux d'eau d'infiltration et de recharge: une approche de la colonne à la parcelle

National audienc