Atmospheric pollutants dispersion simulation

La difusión es el flujo neto causado por el desplazamiento de los contaminantes gaseosos en la atmósfera, provocando la dilución y reducción de los gradientes de concentración. Los modelos de calidad de aire consideran los procesos físicos y químicos que rigen a los contaminantes cuando se dispersan y reaccionan en la atmósfera. La simulación es un método de resolución de ecuaciones que representan un fenómeno que, posteriormente, se relaciona con la situación real. El método gaussiano emplea la ecuación de distribución normal gaussiana para determinar la variación de las concentraciones de contaminantes en la pluma, mediante cálculos relativamente simples que requieren los coeficientes de dispersión horizontal (σy) y vertical (σz). La simulación para determinar concentraciones de contaminantes como el dióxido de azufre (SO2) y monóxido de carbono (CO), fue realizada con el programa MATLAB para ciertas condiciones de velocidad de viento (v), altura de chimenea (h) y clase de estabilidad en áreas rurales. Se realizaron, además, simulaciones empleando el modelo de calidad de aire de EPA: SCREEN3 MODEL * VERSION DATED 96043 * para comparar los valores de las concentraciones de SO2 y CO, calculadas con MATLAB.Diffusion is the net flow caused by the displacement of the gaseous pollutants in the atmosphere, diluting and reducing their concentration gradients. The air quality models consider the physical and chemical processes that govern when contaminants disperse and react in the atmosphere. Simulation is a method of solving equations which represent a phenomenon that, subsequently, relates to the actual situation. The Gaussian‘s method employs the Gaussian‘s normal distribution equation to determine the variation of the concentrations of pollutants in the plume, by relatively simple calculations requiring horizontal dispersion coefficients (σy) and vertical (σz). The simulation to determine concentrations of pollutants such as sulfur dioxide (SO2) and carbon monoxide (CO) was performed with the MATLAB program for certain conditions of wind speed (v), stack height (h) and stability class in rural areas. It also conducted simulations using the model EPA air quality: * VERSION DATED MODEL SCREEN3 96,043 * to compare the values of SO2 and CO concentrations, calculated with MATLAB.Fil: Manzur, Maria Eugenia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Benzal, María Graciela. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Matematica; ArgentinaFil: Gonzalez, Silvia Nelina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentin

Characterization of thick yogurt made from sheep milk

En Argentina, el destino principal de la leche ovina es la elaboración de quesos. Sin embargo, su alto contenido en extracto seco la convierte en una materia prima de excelente calidad para la elaboración de yogur. Se estudió la elaboración de yogur con leche ovina conservada por un periodo de dos meses a -18°C. Luego de ser descongelada (baño termostático a 40 ºC), se analizó microbiológicamente y se procedió con la elaboración de yogur firme por método tradicional, se evaluaron sus características microbiológicas, físico-químicas y composicionales. Finalmente, se realizó un análisis sensorial (juez afectivo). Los análisis de la leche ovina descongelada no mostraron variación en sus características físico-químicas al compararlos con los de leche ovina fresca; sin embargo, se observó un alto recuento de mesófilos viables, debido al proceso de descongelación. El yogur obtenido presentó un 7,58% de proteínas y 10,03% de grasa. En cuanto a sus características microbiológicas, se obtuvieron valores menores a 1 UFC/g para coliformes, hongos y levaduras, pudiéndose considerarlo como un producto microbiológicamente inocuo. En cuanto a los datos del análisis sensorial, se obtuvo una aceptabilidad alta (94%) del producto. La leche ovina congelada sería adecuada para la elaboración de un yogur muy aceptable.Dairy sheep production is growing in Argentina and sheep milk is mainly used for making cheese. However, its high dry matter content makes it attractive for yogurt production. The main objective of this work was to study the elaboration process of yogurt made from sheep milk stored for two months at -18 ° C. After thawing (thermostatic bath at 40 ° C), a microbiological analysis was performed and yogurt was elaborated. Then its microbiological, physicochemical and compositional haracteristics were evaluated. Acceptability of the final product was determined by a sensory test. Thawed sheep milk showed no variation in compositional and physicochemical characteristics compared to fresh sheep milk; however, a high count of viable mesophilic due to the thawing process was observed. Yogurt compositional analysis showed values of 7.58% protein and 10.03% fat. As to microbiological characteristics, values lower than 1 CFU/g for coliforms, fungi and yeast were observed. During the sensory test, high acceptability (94%) was observed. Results suggest that frozen sheep milk may be a suitable candidate for elaborating a highly acceptable yogurt.Fil: Belaunzarán, María Josefina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Tecnología de los Alimentos; ArgentinaFil: Iezzi, Sebastián Juan. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vega, María Fernanda. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Tecnología de los Alimentos; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia Para Lactobacilos (i); Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Farmacia. Catedra de Salud Publica; Argentin

Estudio aleatorizado, doble-ciego y controlado por placebo del efecto de un simbiótico sobre el tránsito intestinal en mujeres adultas sanas

Objetivo: Evaluar el tiempo de tránsito intestinal (TTI) antes y después de consumir yogur con Bifidobacterium BB12, Lactobacillus casei CRL 431 y fibra (simbiótico). Métodos: Estudio prospectivo, cruzado, aleatorizado y doble-ciego con 4 fases: preparación (tiempo 0); intervención (yogur o simbiótico; tiempo 1 y tiempo 3); sin intervención (tiempo 2). Evaluamos la frecuencia de defecación y el consumo de fibra. El TTI se estimo con rojo carmín. Resultados: La edad promedio fue 40,7 años (n = 102 mujeres sanas; 83 completaron el estudio). En mujeres con TTI inicial (TTII) > 48 h que consumieron el simbiótico, el TTII y el TTI final (TTIF) fue 86,9 ± 38,5 h y 51,2 ± 29,8 h (-40,9%), respectivamente, comparado con el de mujeres que consumieron el producto control (TTII, 80,8 ± 31,7 h; TTIF, 69,5 ± 31,5 h; -13,8%) (p = 0,001). En mujeres con constipación funcional que consumieron el producto control, el TTII fue 57,0 ± 30,0 h, valor que aumentó 2,8 h después de consumir el producto (TTIF, 59,8 ± 30,2 h; + 4,9%). El TTII en mujeres que consumieron el simbiótico fue 69,0 ± 49,6 h, registrándose una disminución de 27,5% 19 h después del consumo (TTIF, 50,0 ± 27,5 h; p = 0,023). La normalización de la flora láctica entérica fue significativamente mayor en mujeres que consumieron el simbiótico al inicio (p < 0,1). Conclusión: El TTI disminuyó significativamente después de consumir el simbiótico. Dicho efecto beneficioso fue más evidente en mujeres con TTII ≥ 48 h y constipación funcional.Objective: To evaluate oro-cecal intestinal transit time (ITT) before and after administration of a dairy product containing Bifidobacterium BB12, Lactobacillus casei CRL 431 and fiber in healthy women. Methods: A prospective, randomised, double-blind and cross-over study with a 4-phase design (run-in: time 0 [T0], two intervention periods: time 1 [T1] and time 3 [T3] and a wash-out: time 2, [T2]) was performed. Participants were asked about bowel movement and fiber consumption. ITT was assessed by the carmine red dye method. Results: Mean age was 40.7 years (n = 102 healthy women; 83 completed the study). In women with initial ITT (IITT) > 48 h consuming the synbiotic product, mean IITT and final ITT (FITT) was 86.9 ± 38.5 h and 51.2 ± 29.8 h (-40.9%), as compared to women consuming the control yoghurt (IITT, 80.8 ± 31.7 h; FITT, 69.5 ± 31.5 h; -13.8%) (p = 0.001). IITT in women with functional constipation consuming the control yoghurt was 57.0 ± 30.0 h; such figure increased 2.8 h after yoghurt consumption (FITT, 59.8 ± 3 0.2 h; +4.9%). Conversely, IITT in women who received the synbiotic yoghurt was 69.0 ± 49.6 h, with a -27.5% decrease 19 h later (FITT, 50.0 ± 27.5 h; p = 0.023). Enteric lactic flora stabilization was significantly higher in women who initially consumed the synbiotic product (p < 0.1). Conclusion: ITT decreased significantly after consumption of the synbiotic product. Such beneficial effect was more evident in women with IITT ≥ 48 h and with functional constipation.Fil: Malpeli, Agustina. Provincia de Buenos Aires. Ministerio de Salud. Comisión de Investigaciones Científicas. Hospital de Niños de La Plata. Instituto de Desarrollo e Investigaciones Pediátricas “Prof. Dr. Fernando E. Viteri”; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Vicentin, Dimas. SanCor Cul. Departamento de Investigación, Innovación y Desarrollo; ArgentinaFil: Apas, Ana Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: González, Horacio F.. Provincia de Buenos Aires. Ministerio de Salud. Comisión de Investigaciones Científicas. Hospital de Niños de La Plata. Instituto de Desarrollo e Investigaciones Pediátricas “Prof. Dr. Fernando E. Viteri”; Argentin

Fermented Milks from Small Ruminant: Effect on Metabolism and Immune Status of Mice Fed Mild Caloric Restricted Diet

The purpose of this study was to evaluate the effect of the administration of Fermented Goat´S Milks (FGMs) in mice fed mild caloric restricted diet. The ability of these FGMs to ameliorate immune and metabolic parameters related to mild caloric restriction was studied. We also analyzed if the time (45 or 90 days) of caloric restriction have influence on leptin secretion, IgA+ cells number and phagocytic activity of peritoneal macrophages, and also test the correlation between these parameters. Mice were fed with a mild caloric restricted diet during 45 or 90 days. After these periods of caloric restriction, mice were refed with balanced conventional diet (BCD) plus goat´s milks or BCD plus goat´s milks fermented with Lactobacillus rhamnosus CRL1425 (FGM-Lr) or Lactobacillus casei CRL431 (FGM-Lc). All renutrition diets induced an increase of serum glucose, triglycerides, total proteins and leucocytes cells. A decrease in cholesterol levels was observed after the diets. FGM-Lr induced lower triglycerides values than others diets. All FGMs restore IgA+ cells in intestinal mucosa, and the FGM-Lc group had higher positive cells number than the ad libitum control. Furthermore, phagocytic activity of peritoneal macrophages only increased in mice fed with FGM-Lc. Mild caloric restriction induced a decrease on leptin circulating values at 90 days. The renutrition with FGM-Lr lead to lower leptin levels than mild caloric restriction controls, while FGM-Lc induced higher leptin levels. Positive correlation between serum leptin concentration and immune parameters was observed in all groups under study. We showed that leptin levels could positively predict the immune mucosal competence in mild caloric restricted mice. These results suggest that the FGMs are able to modulate, in different way, serum leptin levels, IgA+ cells number and phagocytic activity. Furthermore, FGM-Lc could be more effective for nutritional treatment in malnutrition status.Fil: Fabersani Marrades, Mario Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto de Quimica del Noroeste; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Valdez, C.. Universidad Nacional de Tucumán; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Gauffin Cano, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Universidad del Norte Santo Tomás de Aquino; Argentin

Prevalence of intestinal parasitic infection among children from a shanty town in Tucuman, Argentina

A parasitological survey was conducted in children living in a poor area next to a drainage channel, in Tucumán province, Argentina. Stool specimens from 115 children were collected and samples were analyzed by direct microscopy examination and concentration techniques. The prevalence rate of intestinal parasites infection was high (78.3%) and Blastocystis hominis was the most frequent protozoan parasite found (68.9%) followed by Giardia intestinalis (33.3%), Entamoeba coli (24.4%), Endolimax nana (12.2%), Chilomastix mesnili (5.6%) and Iodamoeba bütschlii (2.2%). Ascaris lumbricoides was the most prevalent intestinal helminth, with an infection frequency of 38.9%, followed by Trichuris trichiura (13.3%), Strongyloides stercoralis (13.3%), Hymenolepis nana (7.8%) and Enterobius vermicularis (3.3%). Multiple parasitic infections were also high, affecting 71.1% of infected population. These results indicate that sanitary policies, including health care and sanitary education have been inadequate for the control of intestinal parasitism in this high-risk population. Implementation of sanitation programs is a basic need and a joint collaboration between public servant and health professionals should be a priority.Fil: Dib, Julian Rafael. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fernandez Zenoff, Maria Veronica. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Oquilla, Juana del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Lazarte, S.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentin

Scindapsus aureus as potential biomarker of polluted environment

Living organisms are subject to different environmental effects, including physical, biological or chemical factors. In front of pollution, which can be defined as an aggression, the organisms must be adapted to survive. In microsomal fraction of cells, there is an oxidase enzymatic complex, whose central element is the cytochrome P450, able to eliminate certain harmful substances. The main action of P450 system is to introduce a functional group to foreign lipophilic compound, which deveins water-soluble and it excretion was facilitated. In this study a method, originally designed to measure P450 activity in animal cells, was modified and adapted for application in plant cells from contaminated environments. Scindapsus aureus also namely Epipremnum aureum, is considered between the best plants with air purification ability. The aim of this work was to study the effect of polluted environments on this plant species, to determining its potential use as contamination biomarker. The results indicated that P450 activity was manifested only in saturated cell-free extract dialysates from those plants exposed to high contamination during periods longer than 30 days. The induction of this enzyme, with detoxificant power, could depend of pollutant type, and/or exposure times. Few studies using plants as air contamination biomarker by CO were conducted. The results obtained allow recommend susceptible species for quantifying indirect pollution in offices, laboratories, factories, where the workers are exposed 8 h daily, during several years.Fil: Cruz, Rosa Magdalena. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Farmacia. Cátedra de Salud Publica; ArgentinaFil: Fuentes de Curia, Maria Elena. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Farmacia. Cátedra de Salud Publica; ArgentinaFil: Khouri, Eduardo Alejandro. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Farmacia. Cátedra de Salud Publica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rollan, Graciela Celestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gonzalez, Silvia Nelina. Universidad Nacional de Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Biotecnología Farmacéutica y Alimentaria; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Farmacia. Cátedra de Salud Publica; Argentin

Parasitological Survey of Schoolchildren from a High-Altitude Community from Tucumán Province, Argentina. Remarkable Low Detection of Soil-Transmitted Helminths in Comparison with Coastal Communities

The prevalence of intestinal parasitic infections among schoolchildren in Colalao del Valle, a high-altitude community in Tucumán province, Argentina, was investigated. The data revealed a high prevalence of parasitism (79.7%) with no significant differences in distribution by sex or age. Protozoa infections were the most common with Blastocystis hominis being the most prevalent (62.5%), followed by Giardia lamblia (29.7%), Endolimax nana (15.6%), Entamoeba coli (12.5%) and Iodamoeba bustchili (3.1%). Interestingly, there was an absence of soil-transmitted helminths among the studied population which could be related to climate (variable temperatures, moderate rainfall) and soil type (clay).Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Fernandez Zenoff, Maria Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Oquilla, Juana del Valle. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Rudelli, M.. Ministerio de Salud de la Nación; ArgentinaFil: Lazarte, S.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentin

Specific Strains of Lactic Acid Bacteria Differentially Modulate the Profile of Adipokines In Vitro

Obesity induces local/systemic inflammation accompanied by increases in macrophage infiltration into adipose tissue and production of inflammatory cytokines, chemokines, and hormones. Previous studies have shown that probiotics could improve the intestinal dysbiosis induced by metabolic diseases such as obesity, diabetes, and metabolic syndrome. Microorganisms could (directly or indirectly) affect adipokine levels due to their capacity to induce translocation of several intestinal microbial antigens into systemic circulation, which could lead to metabolic endotoxemia or produce immunomodulation in different organs. The aim of the present study was to select non-inflammatory lactic acid bacteria (LAB) strains with the capacity to modulate adipokine secretion by the adipose tissue. We wish to elucidate the role of potential probiotic strains in the regulation of the cross talking between immune cells such as macrophages and adipose cells. Mouse macrophage cell line RAW 264.7 was used for evaluating the ability of 14 LAB strains to induce cytokine production. The LAB strains were chosen based on their previously studied beneficial properties in health. Then, in murine adipocyte culture and macrophage-adipocyte coculture, we determined the ability of these strains to induce cytokines and leptin secretion. Tumor necrosis factor alpha, interleukin 6 (IL-6), IL-10, monocyte chemoattractant protein-1, and leptin levels were measured in cell supernatants. We also performed the detection and quantification of leptin receptor (Ob-Rb) expression in macrophage cell lines stimulated by these LAB strains. Differential secretion profile of cytokines in macrophage cells induced by LAB strains was observed. Also, the levels of Ob-Rb expression diverged among different LAB strains. In LAB-stimulated coculture cells (adipocytes and macrophages), we observed differential production of leptin and cytokines. Furthermore, we detected lower production levels in single culture than cocultured cells. The principal component analysis showed an association between the four clusters of strains established according to their inflammatory profiles and leptin adipocyte production and leptin receptor expression in macrophages. We conclude that coculture is the most appropriate system for selecting strains with the ability to modulate adipokine secretion. The use of microorganisms with low and medium inflammatory properties and ability to modulate leptin levels could be a strategy for the treatment of some metabolic diseases associated with dysregulation of immune response.Fil: Fabersani Marrades, Mario Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Abeijon Mukdsi, Maria Claudia. Universidad del Norte Santo Tomás de Aquino; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Ross, Gloria Romina. Universidad del Norte Santo Tomás de Aquino; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Medina, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: González, Silvia. Universidad Nacional de Tucumán; ArgentinaFil: Gauffin-Cano, Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentin

Preliminary assays for the development of a probiotic for goats

In order to determine probiotic properties, 137 strains of lactic acid bacteria from the feces of Creole goats were screened, only six were resistant to pH 2.0 and bile salts (0.3%). Three strains identified as Lactobacillus and two as Enterococcus showed agglutination with the treated yeast. Between them, Lactobacillus DDL17, DDL19, DDL48 and Enterococcus DDE39 demonstrated high specificity in this test because the correspondent agglutination was inhibited by one sugar, suggesting the presence of a lectin-like structure in their cell walls, which could be due to adhesion ability. Another Enterococcus strain (DDE55) showed low affinity because five sugars inhibited the agglutination of the treated yeasts. The results of hydrophobic properties showed that the strains who were able to agglutinate yeasts presented similar hydrophobic characteristics as hexadecane, xylene and toluene, but high specificity was not related to a high hydrophobicity. Only two strains (Lactobacillus DDL19 and DDL48) showed aggregation with the lowest concentration of ammonium sulfate, complementing the hydrophobicity assay. Only one strain, Lactobacillus DDL48, showed an inhibition against an enteric indicator strain (Salmonella Typhimurium and Escherichia coli O111). This inhibitory action was not affected by the addition of catalase and no inhibition was detected after neutralizing the supernatant culture fluid. These strains could be pre-selected in order to complete studies focused on designing a probiotic for use in goat feed.Fil: Draksler, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Oliver, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin

Fabricación de quesos de cabra con adición de fermentos lácticos autóctonos iniciadores y adjuntos: Impacto en el flavour

El objetivo de este trabajo fue fabricar quesos caprinos con fermentos iniciadores y adjuntos autóctonos regionales a fin de caracterizar la maduración y evaluar su impacto en el flavour del producto. Se fabricaron dos lotes de quesos semiduros. El Lote Control estuvo constituido por el fermento iniciador Lactobacillus bulgaricus ETC2 y el Lote Experimental por dicho fermento con la adición de los fermentos adjuntos Enterococcus faecium BM6 y BM18. Se analizó a los días tres y 60 el desarrollo de microorganismos y al día 60 se efectuó un análisis sensorial. Los resultados mostraron mayor desarrollo de lactococos, lactobacilos y enterococos en los quesos experimentales con adición de fermentos iniciador y adjunto, lo que se expresó sensorialmente entre ambos lotes.Fil: Oliszewski, Ruben. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Departamento de Produccion Animal. Laboratorio de Calidad de Lacteos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; ArgentinaFil: Guzmán, Facundo. Universidad Nacional de Tucuman. Facultad de Agronomia y Zootecnia. Departamento de Produccion Animal. Laboratorio de Calidad de Lacteos; ArgentinaFil: Gonzalez, Silvia Nelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentin