1 research outputs found
Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants
Most of the pepper species of the genus Capsicum have been recalcitrant
to efficient Agrobacterium tumefaciens-mediated stable or transient,
genetic transformation. In the present work, we optimized a protocol
for transient transformation of the Habanero pepper (Capsicum chinense
Jacq.) through the standardization of several experimental factors.
These included the age of the plants, the temperature, the length of
co-cultivation, the application of a negative (vacuum) and/or a
positive (infiltration) pressure, along with micro injection, the use
of acetosyringone during the bacterial culturing, and modification of
the pH during the GUS assay to eliminate the endogenous
\u3b2-glucuronidase activity. The standardized protocol, which yielded
nearly 55% fully transformed leaf explants, was used to successfully
mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as
the C. chinense cDNAs encoding the pathogenesis-related protein 10 and
esterase, respectively