A short-term clinical and microbial evaluation of periodontal therapy associated with amalgam overhang removal

Made available in DSpace on 2019-09-12T16:56:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2006Background: This study evaluated the effect of periodontal therapy plus amalgam overhang removal (PT+AOR) on periodontal status and the presence of Actinobacillus actinomycetemcomitans and Streptococcus mutans and compared two microbiological sampling techniques. Methods: Molar teeth with Class 11 overhang restorations were selected as the test group, and homologous sound teeth were selected as the control group. Periodontal probing depth (PD), clinical attachment level (CAL), and plaque and gingival indices were measured, and restorations were evaluated using radiographs and clinical exploration at baseline (T I) and 90 days after PT+AOR (T2). Microbial samples were taken with dental floss and paper points at T1 and T2. S. mutans and A. actinomycetemcomitans were detected using culture and commercial kits. Results: Mean values of plaque and gingival indices did not show statistically significant differences between test and control teeth between T1 and T2. At T2, the mean CAL decreased among test teeth, and the number of test teeth positive for A. actinomycetemcomitans showed a decrease, whereas positive samples for S. mutans increased. At T1, an association between the two sampling techniques for both bacteria was found. At T2, dental floss presented low sensitivity values for A. actinomycetemcomitans, whereas paper points showed low sensitivity for S. mutans. Conclusions: PT+AOR showed a beneficial effect on clinical parameters and a decrease of A. actinomycetemcomitans. At T1, both sampling techniques showed appropriate results. At T2, the use of paper points was a more sensitive sampling technique for isolate A. actinomycetemcomitans, whereas dental floss was found to be an alternative sampling method for isolateUniversidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazil; Guarulhos Univ, Dent Res Ctr, Dept Periodontol, Sao Paulo, Brazil; Pfizer Consumer Healthcare, Oral Care Res & Dev, Morris Plains, NJ US

Detection of Periodontal Pathogens in Oral Mucous Membranes of Edentulous Individuals

Made available in DSpace on 2019-09-12T16:56:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2008Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade de Taubaté (Unitau)Background: The purpose of this study was to investigate the colonization of Campylobacter rectus, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Prevotella intermedia, and Tannerella forsythia (previously T. forsythensis) in the tongue and cheek of newborns and elderly individuals with no teeth. Methods: Seventy-four edentulous subjects were included in this cross-sectional study. Microbiologic samples were taken from the dorsum of the tongue and cheek mucosa of all individuals and analyzed using a bacterial DNA-specific polymerase chain reaction. Results: C. rectus was the most prevalent species in both groups (20.9% in the cheek of newborns, and 77.4% in the tongue of elderly subjects). P. gingivalis and P. intermedia were not detected in any of the 43 newborns; however, P. gingivalis was recovered from the tongue and cheek (3.2%) of elderly individuals, whereas P. intermedia was detected in the tongue (9.6%) and cheek (3.2%) of elderly individuals. T. forsyth synthia was detected in newborns as well as elderly individuals, although the highest prevalence was observed in the tongue of newborns (6.9%) and elderly (9.6%) individuals. A. actinomycetemcomitans was not found in the tongue of newborns, but we observed A. actinomycetemcomitans in the cheek (2.3%) of newborns and in the tongue (12.9%) and cheek (6.4%) of elderly patients. Conclusions: Although we did not detect P. gingivalis and P. intermedia in newborns, periodontal pathogens could be detected from the oral mucous membranes of edentulous individuals. Our results suggest that major attention should be paid to edentulous individuals as an important measure in the prevention of the initial colonization of natural teeth and dental implants by periodontal pathogens. J Periodontol 2008; 79:7962-1965.[Cortelli, Jose Roberto; Aquino, Davi Romeiro; Cortelli, Sheila Cavalca; Nobre Franco, Gilson Cesar; Fernandes, Camila Borges; Goncalves Roman-Torres, Caio Vinicius] Universidade de Taubaté (Unitau), Dept Dent[Costa, Fernando Oliveira] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazi

Diminished Treatment Response of Periodontally Diseased Patients Infected with the JP2 Clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2009National Institute of Dental and Craniofacial Research (NIDCR)This longitudinal study evaluated the response to periodontal treatment by subjects infected with either JP2 (n = 25) or non-JP2 (n = 25) Aggregatibacter (Actinobacillus) actinomycetemcomitans. Participants were treated during the first 4 months by receiving (i) scaling and root planing, (ii) systemic antibiotic therapy, and (iii) periodontal surgery. Probing depth (PD), clinical attachment level (CAL), and gingival and plaque indices (GI and PI, respectively) were monitored at baseline and at 12 months, along with DNA-PCR-based subgingival detection of JP2 or non-JP2 A. actinomycetemcomitans. At baseline, PD, CAL, and GI scores were statistically higher in the JP2 strain-positive group than the non-JP2-strain-positive group. At 12 months, PD, CAL, and GI scores had decreased significantly for both groups, but the reduction rates of PD and CAL were higher in the non-JP2-strain-positive group. Among JP2-strain-positive patients in the baseline, patients who remained JP2 strain positive at 12 months showed significantly higher GIs than did the patients who had lost the detectable JP2 clone. Patients who remained JP2 strain positive at 12 months appeared to be more resistant to mechanical-chemical therapy than did those who were still non-JP2 strain positive, while the elimination of JP2 A. actinomycetemcomitans remarkably diminished gingival inflammation. Early identification and elimination of the JP2 clone of A. actinomycetemcomitans will enable practitioners to effectively predict the outcome of treatments applied to periodontal patients.[Cortelli, Sheila Cavalca; Aquino, Davi Romeiro; Goncalves Roman-Torres, Caio Vinicius; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dent Res Div, Dept Periodontol[Costa, Fernando Oliveira] Univ Fed Minas Gerais, Dent Res Div, Dept Periodontol, Belo Horizonte, MG, Brazil[Kawai, Toshihisa; Ohara, Kazuhisa] Forsyth Inst, Dept Immunol, Boston, MA USA[Nobre Franco, Gilson Cesar] Universidade de Taubaté (Unitau), Dept Oral Biol, Dent Res Di

Prevalence and distribution of serotype-specific genotypes of Aggregatibacter actinomycetemcomitans in chronic periodontitis Brazilian subjects

Made available in DSpace on 2019-09-12T16:53:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2010Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Objective: Previous studies have suggested that Aggregatibacter actinomycetemcomitans is involved in the aetiology of aggressive periodontitis as well as chronic periodontitis. In addition, some authors have also reported that serotype-specific antigens of A. actinomycetemcomitans determine the severity of disease. This study aimed to elucidate the prevalence of A. actinomycetemcomitans and the distribution of A. actinomycetemcomitans serotypes in Brazilian subjects with chronic periodontitis. Design: A total of 486 individuals were enrolled in this survey. All patients received clinical examinations that included periodontal pocket depth, clinical attachment loss, plaque, and gingival indexes. Subgingival samples were taken for microbial analysis. The genomic DNA of A. actinomycetemcomitans was provided by PCR. Results: Out of 486 subjects examined, A. actinomycetemcomitans was isolated in 85 (17.5%) individuals. Out of 85 positive samples, 68 were infected by at least 1 serotype, 7 by mixed infection, and 10 were non-serotyped. Serotypes d and f were not detected. Serotype c showed the highest prevalence (52.9%), followed by serotype a (31.8%). Conclusions: Intragroup analysis revealed that, in slight/moderate periodontitis, serotypes c and a were significantly more prevalent than serotypes b and d-f; the prevalence of serotype c in severe periodontitis was significantly greater than that of serotypes a and b. Our data were similar in Asian and Eurasian populations. (C) 2010 Elsevier Ltd. All rights reserved.[Goncalves Roman-Torres, Caio Vinicius; Aquino, Davi Romeiro; Cortelli, Sheila Cavalca; Nobre Franco, Gilson Cesar; dos Santos, Juliana Guimaraes; Holzhausen, Marinella; Cortelli, Jose Roberto] Universidade de Taubaté (Unitau), Dept Periodontol & Prevent Dent, Dent Res Div, BR-12020330 Taubate, SP, Brazil[Corraini, Priscila] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, BR-05508900 Sao Paulo, Brazil[Diniz, Marina Goncalves; Gomez, Ricardo Santiago] Univ Fed Minas Gerais, Dept Mol Biol, Sch Dent, BR-31270901 Belo Horizonte, MG, Brazi