Lessons We Learned Designing and Building the Chandra Telescope

2014 marks the crystal (15th) anniversary of the launch of the Chandra Xray Observatory. This paper offers some of the major lessons learned by some of the key members of the Chandra Telescope team. We offer some of the lessons gleaned from our experiences developing, designing, building and testing the telescope and its subsystems, with 15 years of hindsight. Among the topics to be discussed are the early developmental tests, known as VETAI and VETAII, requirements derivation, the impact of late requirements and reflection on the conservatism in the design process

Overexpression of YAP1 induces immortalization of normal human keratinocytes by blocking clonal evolution.

YAP1 is a transcriptional co-activator able to bind several transcription factors. YAP1 was termed a candidate oncogene after it was shown to be in human chromosome 11q22 amplicon; besides the genomic amplification, several experiments indicated that it has oncogenic function. However, YAP1 was also reported to be a tumor suppressor as its gene locus is deleted in some breast cancers. To clarify the role of this protein in the physiology of rapidly renewal cells, we investigated YAP1 in human keratinocytes. Here, we show that YAP1 overexpression in primary human keratinocytes blocks clonal evolution and induces cell immortalization, but not malignant transformation. YAP1 overexpression led to an increase in cell proliferation, colony forming efficiency and holoclone percentage. Cells escaped from senescence, immortalized but still remained unable to grow in soft agar or express mesenchymal markers, suggesting that YAP1 overexpression is not sufficient to promote a complete epithelial-mesenchymal transition and tumorigenic transformation. Protein analysis showed an increase in epithelial proliferation markers and a decrease in epithelial differentiation markers. The expression of LEKTI, a late differentiation marker, dramatically dropped to undetectable levels. Taken together, these data suggest that YAP1-overexpressing keratinocytes are maintained in the proliferative compartment

The Design and Capabilities of the EXIST Optical and Infra-Red Telescope (IRT)

The Infra-Red Telescope is a critical element of the EXIST (Energetic X-Ray Imaging Survey Telescope) observatory. The primary goal of the IRT is to obtain photometric and spectroscopic measurements of high redshift (> or =6) gamma ray reaching to the epoque of reionization. The photometric and spectral capabilities of the IRT will allow to use GRB afterglow as probes of the composition and ionization state of the intergalactic medium of the young universe. A prompt follow up (within three minutes) of the transient discovered by the EXIST makes IRT a unique tool for detection and study of these events in the infrared and optical wavelength, which is particularly valuable at wavelengths unavailable to the ground based observatories. We present the results of the mission study development on the IRT as part of the EXIST observatory. Keywords: infrared spectroscopy, space telescope, gamma ray bursts, early univers