The Effect of Vincristine Sulphate on the Axoplasmic Flow of Proteins in Cultured Sympathetic Neurons

The effect of vincristine sulphate on the axoplasmic flow of labelled proteins in neurites of chick embryo sympathetic neurons growing in tissue culture was studied by autoradiography. In control neurons most of the 3H-proteins synthesized during a 90-min pulse with a 3H-amino acid were localized in cell bodies. There was a diminishing gradient of labelled proteins in the neurites which was highest in portions adjacent to the cell bodies and lowest at the periphery. During a physiological chase there was a gradual increase in the amount of label in the neurites, so that after a 15-h chase even the most peripheral portions were well labelled. This indicates that a portion of the labelled proteins synthesized in the cell bodies are transported peripherally into the neurites. The centrifugal movement of labelled proteins in neurites was markedly decreased when cells were grown in medium containing 10 µg/ml vincristine sulphate. After a 15-h chase in the presence of drug only a small amount of label was in the peripheral portion of the neurites. Treatment with vincristine did not decrease the rate of amino acid incorporation or alter the rate of protein turnover during the course of the experiment. Thus an explanation of the results based on an altered rate of total cell protein synthesis or degradation is unlikely. The capacity of sympathetic neurons to take up and concentrate exogenous [3H]norepinephrine in their neurites was only slightly reduced by vincristine. This indicates that at least some cellular activities requiring metabolic energy are relatively unaffected by the interruption in axoplasmic flow caused by vincristine and that the mechanism by which vincristine interferes with axoplasmic flow does not involve general cellular toxicity. The major morphological differences between control and vincristine-treated neurons were the absence of microtubules and the presence of crystal-like structures within the cells. The relationship between the effect of vincristine on the axoplasmic flow of proteins and the arrangement of the microtubule system is discussed

On the quantum probability flux through surfaces

We remark that the often ignored quantum probability current is fundamental for a genuine understanding of scattering phenomena and, in particular, for the statistics of the time and position of the first exit of a quantum particle from a given region, which may be simply expressed in terms of the current. This simple formula for these statistics does not appear as such in the literature. It is proposed that the formula, which is very different from the usual quantum mechanical measurement formulas, be verified experimentally. A full understanding of the quantum current and the associated formula is provided by Bohmian mechanics.Comment: 15 pages, 3 figures, revised and more detailed version, to be published in Journal of Statistical Physics, August 9

Development of advanced fabrication techniques for regeneratively cooled thrust chambers by the gas-pressure-bonding process Final report, 29 Jun. 1967 - 30 Apr. 1970

Production of regeneratively cooled rocket thrust chambers by removable tooling and subsequent hot isostatic pressing in gas autoclav

Synthetic aperture radar target simulator

A simulator for simulating the radar return, or echo, from a target seen by a SAR antenna mounted on a platform moving with respect to the target is described. It includes a first-in first-out memory which has digital information clocked in at a rate related to the frequency of a transmitted radar signal and digital information clocked out with a fixed delay defining range between the SAR and the simulated target, and at a rate related to the frequency of the return signal. An RF input signal having a frequency similar to that utilized by a synthetic aperture array radar is mixed with a local oscillator signal to provide a first baseband signal having a frequency considerably lower than that of the RF input signal

Abelian Gauge Theory in de Sitter Space

Quantization of spinor and vector free fields in 4-dimensional de Sitter space-time, in the ambient space notation, has been studied in the previous works. Various two-points functions for the above fields are presented in this paper. The interaction between the spinor field and the vector field is then studied by the abelian gauge theory. The U(1) gauge invariant spinor field equation is obtained in a coordinate independent way notation and their corresponding conserved currents are computed. The solution of the field equation is obtained by use of the perturbation method in terms of the Green's function. The null curvature limit is discussed in the final stage.Comment: 10 pages, typos corrected, reference adde

Carbon monoxide pollution experiment

The experiment is designed to obtain data for the investigation of mechanisms by which CO is removed from the earth's atmosphere. The approach uses an orbiting platform to remotely map global CO concentrations and determine vertical CO profiles using a correlation interferometer measurement technique. The instrument is capable of measuring CO over the range of expected atmospheric burdens and of measuring trace atmospheric constituents

First Order Actions and Duality

We consider some aspects of classical S-duality transformations in first order actions taken into account the general covariance of the Dirac algorithm and the transformation properties of the Dirac bracket. By classical S-Duality transformations we mean a field redefinition that interchanges the equations of motion and its associated Bianchi identities. By working from a first order variational principle and performing the corresponding Dirac analysis we find that the standard electro-magnetic duality can be reformulated as a canonical local transformation. The reduction from this phase space to the original phase space variables coincides with the well known result about duality as a canonical non local transformation. We have also applied our ideas to the bosonic string. These Dualities are not canonical transformations for the Dirac bracket and relate actions with different kinetic terms in the reduced space.Comment: accepted for publication in IJMP

Are All Particles Identical?

We consider the possibility that all particles in the world are fundamentally identical, i.e., belong to the same species. Different masses, charges, spins, flavors, or colors then merely correspond to different quantum states of the same particle, just as spin-up and spin-down do. The implications of this viewpoint can be best appreciated within Bohmian mechanics, a precise formulation of quantum mechanics with particle trajectories. The implementation of this viewpoint in such a theory leads to trajectories different from those of the usual formulation, and thus to a version of Bohmian mechanics that is inequivalent to, though arguably empirically indistinguishable from, the usual one. The mathematical core of this viewpoint is however rather independent of the detailed dynamical scheme Bohmian mechanics provides, and it amounts to the assertion that the configuration space for N particles, even N ``distinguishable particles,'' is the set of all N-point subsets of physical 3-space.Comment: 12 pages LaTeX, no figure