DNA investigation in the exhaled breath condensate (EBC) in non-small cell lung cancer

Aim: Exhaled breath condensate (EBC), one of the materials which is used to detect mutations in the early period, is collected by completely non-invasive a technique which has no risk for the patient.  We aimed to investigate whether EBC samples are suitable for the detection of DNA or not in non-small cell lung cancer (NSCLC) and control patients.    Methods: 26 patients with NSCLC and 20 patients without lung cancer were included in the study. EBC procedure was performed by the help of Eco Screen- Jaeger device in 10-15 minutes during breathing at the tidal volume. DNA was isolated using tissue spin-column DNA isolation kit in the collected EBC.         Results: DNA amount was twofold high in the NSCLC group than non-cancer patients in spite of short time (p>0.05). However, in cancer group DNA amount was found lower in patients with endobronchial lesions than without endobronchial lesions (p>0.05). Although, there was no relationship between DNA amount and all of EBC collection time, collected sample amount and expiration air volume in the cancer group, a positive relations was detected between DNA amount and EBC collection time in the non-cancer group.   Conclusıon: This may be explained by the pathological changes which occur at the cellular level in the lungs during cancer development process. However, it may also result from relative decrease which develops from redundancy of EBC volume in the non-cancer group. The source of DNA in EBC may be considered to be pathological changes resulting from the systemic inflammatory response, apart from the localized lesion in the lungs

HLA B-27 Subtypes in Turkish Patients with Spondyloarthropathy and Healthy Controls

The frequency and the distribution of HLA-B27 subtypes in spondylarthropathy (SpA) patients and controls were investigated in a sample Turkish population. B27 subtyping was performed by PCR-SSP method in two groups: 49 unrelated HLA-B27 positive Turkish patients with the diagnosis of SpA according to the European Spondyloarthropathy Study Group Criteria, and 55 HLA-B27 positive healthy controls. The frequency of HLA-B(∗)27 was 2.6% in the Turkish population, and B(∗)2705 was the predominant allele among patients with SpA. The difference was mainly between male patients and male controls The proportion of B(∗)2705 among B27-positive patients and controls was significantly different (P = 0.02). Our study supports other reports from different populations which showed that B(∗)2705 and B(∗)2702 were more frequent in Caucasian patients with SpA

Optimizing the Permutation Flowshop Scheduling Problem by Scatter Search

WOS: 000410806200004Scheduling is one of the decision-making processes that play a critical role in the production and service industries. Flow job scheduling is one of the types of scheduling where "n" job can be processed at "m" machines consequently. When the complexity of this problem increases, obtaining the optimum solution becomes difficult. But, solution can be found near the optimum in these complex problems by using the scatter search which is one of the meta heuristics. Scatter search method which is a branch of the evolutionary approach is an advantageous optimization technique due to producing two or more solution. In this study, the new model is developed for optimizing the permutation flowshop scheduling problem by scatter search

Lung Clear “Sugar” Cell Tumor and Jak V617f Positive Essential Thrombocythemia: A simple Coıncıdence?

The primary clear cell tumor of the lung is an extremely rare benign tumor, which is called “sugar tumor”, because of the large content of glycogen. Here we are presenting essential thrombocythemia and lung clear cell tumor which was not reported before to the best of our knowledge. A 44 years old woman admitted to the clinic with complaint of lassitude lasting for 2 months. In her physical examination the spleen was 3 cm palpable from the costa arch In laboratory findings number of platelet was 1014000 mm³. A 3.5 cm in diameter pulmonary nodule is detected in right upper lobe in the graphy of the lungs. Subsequently  computed tomography  (CT ) of thorax was carried out. Due to the benign features in the display of  the detected nodule, a total excision with curative and diagnostic intentions was performed. Microscopically the tumor were composed of nests of rounded or oval cells with distinct cell borders and optically clear cytoplasm. The nuclei were small. Immunohistochemically the tumor cells expressed HMB-45, NSE and focal S100 antigen. It was diagnosed as clear  “sugar” cell tumor. In conclusion, in lung clear cell tumor, it is important to make evaluation in terms of myeloproliferative disease in adults whose thrombocytosis continue after the treatment

HLA B-27 subtypes in turkish patients with spondyloarthropathy and healthy controls

Abstract. The frequency and the distribution of HLA-B27 subtypes in spondylarthropathy (SpA) patients and controls were investigated in a sample Turkish population. B27 subtyping was performed by PCR-SSP method in two groups: 49 unrelated HLA-B27 positive Turkish patients with the diagnosis of SpA according to the European Spondyloarthropathy Study Group Criteria, and 55 HLA-B27 positive healthy controls. The frequency of HLA-B*27 was 2.6% in the Turkish population, and B*2705 was the predominant allele among patients with SpA. The difference was mainly between male patients and male controls The proportion of B*2705 among B27-positive patients and controls was significantly different (P = 0.02). Our study supports other reports from different populations which showed that B*2705 and B*2702 were more frequent in Caucasian patients with SpA