112 research outputs found
Iodine-Catalyzed Highly Effective Pictet Spengler Condensation: An Efficient Synthesis of Tetrahydro-b-carbolines
Molecular iodine was found to be an efficient catalyst for the twocomponent
Pictet–Spengler condensation of tryptamine with aldehydes, which
leads to the formation of tetrahydro-b-carbolines in high yields
Iodine a simple, effective and inexpensive catalyst for the synthesis of substituted coumarins
Molecular iodine is developed as an e
Neural Greedy Pursuit for Feature Selection
We propose a greedy algorithm to select important features among
input features for a non-linear prediction problem. The features are selected
one by one sequentially, in an iterative loss minimization procedure. We use
neural networks as predictors in the algorithm to compute the loss and hence,
we refer to our method as neural greedy pursuit (NGP). NGP is efficient in
selecting features when , and it provides a notion of feature
importance in a descending order following the sequential selection procedure.
We experimentally show that NGP provides better performance than several
feature selection methods such as DeepLIFT and Drop-one-out loss. In addition,
we experimentally show a phase transition behavior in which perfect selection
of all features without false positives is possible when the training data
size exceeds a threshold
INVESTIGATING THE ROLE OF NF-κB, COX-1, COX-2, COMT, IL-10, IL-6 AND TNF-α IN MODULATING ANTI-NOCICEPTIVE ACTIVITY OF METHANOLIC EXTRACT OF ENTADA PHASEOLOIDES
Objective: To investigate the analgesic activity of methanolic extract of Entada phaseoloides (MEEP) along with its molecular mechanistic pathway.
Methods: Swiss albino female mice and Wistar rats of either sex were administered orally with MEEP extracts (100, 200 and 400 mg/kg) and pentazocine, tramadol and diclofenac sodium, as standard drugs. Following administration, anti-nociceptive activity was evaluated using an acetic acid-induced writhing test, Eddy’s hot plate, and hot water immersion test. Serum was collected for molecular expression of various proteins and genes using Reverse Transcriptase PCR and Western Blotting.
Results: Acetic acid writhing test, a frequently used method to assess peripheral analgesic activity, revealed that MEEP reduced peripherally induced pain in a dose-dependent manner. Likewise, Eddy’s hot plate and hot water immersion methods, often implicated for testing central analgesic activities, showed that MEEP is bestowed with the capability to counteract analgesia in a dose and time-dependent manner. Pro-inflammatory cytokines and factors like COX-2, IL-6, TNF-α, and NF-κB that cause inflammatory responses and pain were significantly reduced, suggesting its analgesic and anti-inflammatory potential. This analgesic and the anti-inflammatory role played by MEEP is also supported by the up-regulation of anti-inflammatory cytokine IL-10 and COMT and COX-1 enzyme demonstrated no significant difference between the groups.
Conclusion: The study revealed the weak peripheral and potent central analgesic property MEEP by modulating pro-inflammatory and anti-inflammatory pathways
Green chemistry approaches to the regioselective synthesis of spiro heterobicyclic rings using iodine as a new and efficient catalyst under solvent-free conditions
Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)
This report describes the cloning and expression of a gene lycopene epsilon cyclase, (LCYE) from Camellia sinensis var assamica which is a precursor of the carotenoid lutein in tea. The 1982 bp cDNA sequence with 1599 bp open reading frame of LCYE was identified from an SSH library constructed for quality trait in tea. 5’ and 3’ RACE (rapid-amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology studies showed that the deduced amino acid sequence of LCYE gene had the highest sequence identity of up to 84% with Vitis vinefera. The cloned gene was successfully expressed in a PET based Escherichia coli expression system. The size of the expressed protein was 59615 Daltons. A suppression subtractive library was constructed using a quality clone H3111 (tester) and a garden series clone T3E3 (driver).Key words: Carotenoid, RACE, heterologous expression, lutein, tea
Transcriptional responses and flavor volatiles biosynthesis in methyl jasmonate-treated tea leaves
- …