Kombucha microbiome as a probiotic: a view from the perspective of post-genomics and synthetic ecology

Probiotics are essential for establishing and maintaining optimal immune health. The probiotic therapy is known from alternative medicine for ages; however, the recent demonstration of the normal microflora to induce innate immunity has introduced the science-based concept of therapeutic application of potentially beneficial probiotic microorganisms for a treatment of functional disorders. Traditionally, probiotics are associated with dairy products, however, novel formulations are needed, first of all, originated from naturally occurring symbiotic microbial communities as the most robust assemblages. Especially, safe and robust probiotics are needed for long-term expeditions, outposts, extraterrestrial permanently-manned bases, where humans are exposed to adverse environmental factors. Kombucha beverage is Symbiotic Culture of Bacteria and Yeast (SCOBY) and associated with health-promoting effects. Kombucha tea/mat is being in use in human livings within millennia as a probiotic drink for healing and health prophylaxis effects, however, new research opportunities promise its «renaissance», going to be used pharmacologically

СИНТЕЗ АЛИФАТИЧЕСКИХ ЭФИРОВ 5-АМИНОЛЕВУЛИНОВОЙ КИСЛОТЫ И ИХ ВЛИЯНИЕ НА РОСТ ФОТОТРОФНЫХ МИКРОВОДОРОСЛЕЙ

Method for preparation of 5-aminolevulinic acid (ALA) aliphatic esters with aliphatic alcohols (С1–С16) is described. Melting points, spectral parameters and chromatographic characteristics for the esters obtained have been determined. Critical micelle concentration values of ALA hexyl ester and higher homologs in aqueous buffered system have been estimated. The stimulatory effect of micelle forming ALA esters at 0.1 mM concentration on the accumulation of phototrophic microalgae Chlorella and Scenedesmus obliquus dry biomass has been established (106–182% of the control).Описан метод получения эфиров 5-аминолевулиновой кислоты (АЛК) с алифатическими спиртами (С1–С16). Определены температуры плавления, спектральные и хроматографические параметры полученных эфиров. Для гексилового эфира АЛК и более высших гомологов рассчитаны значения критических концентраций мицеллообра­ зования (ККМ) в водном буферном растворе. Установлен стимулирующий эффект мицеллообразующих эфиров АЛК при концентрации 0,1 мМ на накопление сухой биомассы фототрофных микроводорослей Chlorella и Scenedesmus obliquus (106–182% к контролю)

Purple non-sulphur photosynthetic bacteria in brewery wastewater during anaerobic digestion

ABSTRACT: Anaerobic digestion of brewery wastewater (BWW), under batch and constant light conditions, provided 70 mL of biogas with 80% CH4 and a COD removal of 64%, corresponding to a yield of 372 m3 biogas/kg COD removed. Additionally, a reddish pigmentation has developed throughout the process due to the growth of purple nonsulphur bacteria (PNSB). Based on morphological, physiological and cultural properties two strains of PNSB belonging to the genus Rhodobacter were isolated. The comparison of the nucleotide sequences of the 16S rRNA genes of the strains with the international databases (NCBI GenBank) made it possible to establish their taxonomical status as Rhodobacter capsulatus and R. azotoformans species.N/