Additional file 1: of Immunologic and virological response to ART among HIV infected individuals at a tertiary hospital in Ghana

Dataset. (XLSX 50 kb

Light micrograph of <i>Leishmania</i> amastigotes in bone marrow aspirate.

<p>Two infected macrophages are stained (large arrows), each with a nucleus (N) and numerous amastigotes (examples with small arrows) within the cytoplasm. The specimen was stained using Wright's stain. The bar represents 20 µm.</p

Giemsa-stained promastigote forms from culture.

<p>A–H, examples showing morphological variation of forms observed, all at the same magnification, bar in F represents 5 µm. Procyclic-like promastigotes can be observed in A (arrows);, leptomonad-like promastigotes in B and C (arrows); nectomonad-like promastigotes in D, E and F; and rosettes and aggregates in G and H.</p

Diagnosis of leishmaniasis by PCR.

<p>Agarose gel electrophoresis showing PCR products using the LeF/LeR primers for <i>Leishmania</i> rRNA ITS-1 <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003339#pntd.0003339-Spanakos1" target="_blank">[17]</a> from patient bone marrow aspirate samples compared to five <i>Leishmania</i> species, 1% gel, stained with ethidium bromide. Lane 1, negative control – no DNA; lane 2, <i>L. donovani</i>; lane 3, <i>L. infantum</i>; lane 4, <i>L. tropica</i>; lane 5, <i>L. major</i>; lane 6, “<i>L. siamensis</i>”PCM2 Trang; lane 7, BM1 is bone marrow collected 2 days after commencement of treatment; lane 8, BM2 is bone marrow collected 14 days after commencement of treatment. Size markers (MW), 100 bp ladder, are as shown.</p

Phylogenetic analysis by ML method of <i>Leishmania</i> strain CM1 using RNA Pol II sequences.

<p>Tree with 25 species of <i>Leishmania</i> and <i>Endotrypanum monterogeii</i> using <i>Trypanosoma brucei</i> as an outgroup, based on alignment of 1191-1206 homologous nucleotide sequences. AM-2004 is <i>Leishmania</i> from Australia, PCM2 is the “<i>L. siamensis</i>” PCM2 Trang isolate. Bootstrap values from 1000 replicates are given at the nodes.</p