Representative SDS-PAGE results for fractionated and non-fractionated samples (normal and CKD).

<p>The figure represents initial urine, flow-through and elution for each of the depletion kits applied. The fractions representing depleted sample and albumin as a common protein depleted by all the kits are marked. I—Initial urine (non-fractionated sample); F—Flow-through fraction; E—Elution. The same protein amounts were loaded onto the gels for initial sample (lane 2 in all cases). Any observed differences in staining intensities are attributed to differences in the silver staining procedure.</p

Characteristics of the applied depletion strategies.

<p>Characteristics of the applied depletion strategies.</p

Protein amounts at different steps of the analysis as estimated by Bradford measurements.

<p>ND: not determined due to measurements being below the limit of detection (i.e. concentration < 0.2 μg/μL).</p

Comparison of the number of peptide identifications, PSMs, search inputs and TICs for normal and CKD sample.

<p>Comparison of the number of peptide identifications, PSMs, search inputs and TICs for normal and CKD sample.</p

Average number of peptides identified per method.

<p>Average number of peptides identified per method.</p

Total number (sum) of identified proteins per depletion strategy for normal and CKD sample (in at least 3, 4 and 5 technical replicates).

<p>For both depleted and non-depleted sample the number of identifications is higher in normal than in CKD urine.</p

Predictors for the development of AKI, accepted in the logistic regression model.

<p>Predictors for the development of AKI, accepted in the logistic regression model.</p

Studied groups baseline characteristics.

<p>Studied groups baseline characteristics.</p

In-hospital survival in iv. rt-PA treated patients with AKI and those without AKI.

<p>In-hospital survival in iv. rt-PA treated patients with AKI and those without AKI.</p

Comparison of parameters at admission between patients who developed vs. those who had not developed AKI, after iv. rt-PA.

<p>Comparison of parameters at admission between patients who developed vs. those who had not developed AKI, after iv. rt-PA.</p