The bone morphogenetic protein antagonist gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein-4

<p><b>Copyright information:</b></p><p>Taken from "The bone morphogenetic protein antagonist gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein"</p><p>BMC Cancer 2006;6():74-74.</p><p>Published online 18 Mar 2006</p><p>PMCID:PMC1459871.</p><p>Copyright © 2006 Namkoong et al; licensee BioMed Central Ltd.</p>WHAHp1–60 and YWHAHp81–247 were prepared using YWHAH cDNA as a template DNA. The GST pull-down assay showed that Three GST-YWHAH constructs, Full-YWHAHp1–247, YWHAHp1–100 and YWHAHp1–80 bind YWHAH, but not YWHAHp1–60 and YWHAHp60–247. The YWHAH binding site for PIG-2 was delineated to be residues 61–80 (black box). () Mapping of YWHAH binding domain of PIG-2. Four GST fusion constructs, Full-PIG-2p1-184, PIG-2p1-144, PIG-2p1-100 and PIG-2p1-67 were prepared using PIG-2 cDNA as a template DNA. The GST pull-down assay showed all constructs, The PIG-2 binding site for YWHAH was delineated to be residues 1–67. Gray box is DAN domain. () Schematic diagram shows X-ray crystallography of YWHAH protein. PIG-2 protein binding site was indicated by arrow