Antioksidativna aktivnost hidrolizata belanceta i njegovih frakcija dobijenih membranskom ultrafiltraciom

Bioactive peptides with different biological properties can be obtained by egg white proteins hydrolysis. In this study we used the high intensity ultrasound pretreatment of the egg white proteins that were then hydrolyzed by different types of proteases in the one-step and two-step procedure. Membrane ultrafiltration into molecular size of 1 kDa, 10 kDa and 30 kDa was used to separated the obtained hydrolzyates and antioxidative activities of obtain fractions were studied. Between fractions less than 1 kDa, containing bioactive peptides, the ultrasound pretreated hydrolyzate obtained by using alcalaseflevorzyme in a two-stage procedure has shown the highest antioxidant activity.Hidrolizom proteina belanceta dobijaju se bioaktivni peptidi koji imaju različita biološka svojstva. U ovom radu korišćena je tehnologija ultrazvuka visokog intenziteta kao pretretman pripreme proteina belanceta koji su zatim hidrolizovani različitim vrstama proteaza u jednostepenom i dvostepenom postupku. Dobijeni hidrolizati su razdvojeni korišćenjem ultrafiltracionih membrana promera 1, 10 i 30 kDa i dobijenim frakcijama je ispitana antioksidativna aktivnost. Među frakcijama veličine manje od 1 kDa koje sadrže bioaktivne peptide, najveću antioksidativnu aktivnost je pokazao ultrazvučno pretretiran hidrolizat nastao delovanjem alkalaza-flevorzima u dvostepenom enzimskom postupku

Effects of altered hepatic lipid metabolism on regulation of hepatic iNOS

An altered hepatic lipid metabolism involves multifactorial pathologies such as hepatic inflammation, insulin resistance and oxidative stress. Immunity has an essential role in the regulation of glucose and lipid metabolism in the liver. Inducible nitric oxide (NO) synthase (iNOS) has been proposed as an important factor that interplays between immunity and energy metabolism and also in the pathogenesis of obesity-linked insulin resistance. In the liver, locally produced NO plays a protective role during inflammation, and the balance of NO protective and cytotoxic effects is very important. This review is focused on understanding the molecular mechanisms of iNOS regulation in the state of altered hepatic lipid metabolism, which is critical for developing new strategies for treatment of hepatic disorders