Bioactive peptides with different biological properties can be obtained by egg white
proteins hydrolysis. In this study we used the high intensity ultrasound pretreatment of
the egg white proteins that were then hydrolyzed by different types of proteases in the
one-step and two-step procedure. Membrane ultrafiltration into molecular size of 1 kDa,
10 kDa and 30 kDa was used to separated the obtained hydrolzyates and antioxidative
activities of obtain fractions were studied. Between fractions less than 1 kDa, containing
bioactive peptides, the ultrasound pretreated hydrolyzate obtained by using alcalaseflevorzyme
in a two-stage procedure has shown the highest antioxidant activity.Hidrolizom proteina belanceta dobijaju se bioaktivni peptidi koji imaju
različita biološka svojstva. U ovom radu korišćena je tehnologija ultrazvuka visokog
intenziteta kao pretretman pripreme proteina belanceta koji su zatim hidrolizovani
različitim vrstama proteaza u jednostepenom i dvostepenom postupku. Dobijeni
hidrolizati su razdvojeni korišćenjem ultrafiltracionih membrana promera 1, 10 i 30 kDa
i dobijenim frakcijama je ispitana antioksidativna aktivnost. Među frakcijama veličine
manje od 1 kDa koje sadrže bioaktivne peptide, najveću antioksidativnu aktivnost je
pokazao ultrazvučno pretretiran hidrolizat nastao delovanjem alkalaza-flevorzima u
dvostepenom enzimskom postupku
