Vocal behavior and the use of social information during roost finding

When selecting feeding, hiding, or resting areas, animals face multiple decisions with different fitness consequences. To maximize efficiency, individuals can either collect personal information, or use information gathered and transmitted by other individuals (social information). Within group living species, organisms often specialize in either generating social information or using information gathered by other groups members. That is the case of the Spix’s disk-winged bat, Thyroptera tricolor. This species uses contact calls during roost finding. Social groups are composed by a mix of vocal and non-vocal individuals and those vocal roles appear to be consistent over time. Moreover, their vocal behavior can predict roost finding in natural settings, suggesting that vocal individuals are capable of generating social information that can be used by other group members. To date, however, we do not know if when presented with social information (contact calls) during roost finding, vocal individuals will make more or less use of these cues, compared to non-vocal individuals. To answer this question, we broadcast contact calls from a roost inside a flight cage to test whether vocal individuals could find a potential roost faster than non-vocal individuals when they encounter sounds that signal the presence of a roost site. Our results suggest that non-vocal individuals select roost sites based primarily on social information, whereas vocal individuals do not rely heavily on social information when deciding where to roost. This study provides the first link between vocal behavior and the use of social information during the search for roosting resources in bats. Incorporating ideas of social roles, and how individuals decide when and where to move based on the use of social information, may shed some light on these and other outstanding questions about the social lives of bats.Universidad de Costa Rica/[560-B8-015]/UCR/Costa RicaUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de BiologíaUCR::Sedes Regionales::Sede del Su

Vocal behavior and the use of social information during roost finding

When selecting feeding, hiding, or resting areas, animals face multiple decisions with different fitness consequences. To maximize efficiency, individuals can either collect personal information, or use information gathered and transmitted by other individuals (social information). Within group living species, organisms often specialize in either generating social information or using information gathered by other groups members. That is the case of the Spix’s disk-winged bat, Thyroptera tricolor. This species uses contact calls during roost finding. Social groups are composed by a mix of vocal and non-vocal individuals and those vocal roles appear to be consistent over time. Moreover, their vocal behavior can predict roost finding in natural settings, suggesting that vocal individuals are capable of generating social information that can be used by other group members. To date, however, we do not know if when presented with social information (contact calls) during roost finding, vocal individuals will make more or less use of these cues, compared to non-vocal individuals. To answer this question, we broadcast contact calls from a roost inside a flight cage to test whether vocal individuals could find a potential roost faster than non-vocal individuals when they encounter sounds that signal the presence of a roost site. Our results suggest that non-vocal individuals select roost sites based primarily on social information, whereas vocal individuals do not rely heavily on social information when deciding where to roost. This study provides the first link between vocal behavior and the use of social information during the search for roosting resources in bats. Incorporating ideas of social roles, and how individuals decide when and where to move based on the use of social information, may shed some light on these and other outstanding questions about the social lives of bats

Cooperative signaling behavior of roost location in a leaf-roosting bat

Research suggests that social calls are important for conveying information about food and roost location in bats. However, no studies have specifically documented calls that are used to actively attract conspecifics to roosting locations. Here we describe the cooperative signaling behavior of roost location towards flying conspecifics in Spix’s disc-winged bat (Thyroptera tricolor), a species that uses a highly ephemeral roosting resource. Two types of calls were recorded during field experiments; one from flying individuals termed “inquiry calls”, and another from roosting bats termed “response calls”. Inquiry calls were emitted by flying bats immediately upon release, and quickly elicited production of response calls from roosting individuals. Most flying bats entered the roost when roosting individuals responded, while very few bats entered the roost in the absence of a response. During playback experiments, we found significant differences in response rates among individuals, which could be caused by diverse intrinsic and extrinsic factors. In addition, results of our ongoing field studies suggest that the cooperative signaling behavior of roost location is important in maintaining social cohesion, and that the use of a larger home range when resources are scarcer may decrease group stability by hindering communication.UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Variation in echolocation call frequencies in two species of free-tailed bats according to temperature and humidity

Bats can actively adjust their echolocation signals to specific habitats and tasks, yet it is not known if bats also modify their calls to decrease atmospheric attenuation. Here the authors test the hypothesis that individuals emit echolocation calls ideally suited to current conditions of temperature and humidity. The authors recorded two species, Molossus molossus and Molossops temminckii, in the field under different conditions of humidity and temperature. For each species, two calls were analyzed: the shorter frequency modulated (FM) signals that bats emitted as they approached the recording microphone, and the longer constant frequency (CF) calls emitted thereafter. For each signal, the authors extracted peak frequency and duration, and compared these parameters among species, call type, and environmental conditions. The authors' results show significant differences in peak frequency and duration among environmental conditions for both call types. Bats decreased the frequency and increased duration of CF calls as atmospheric attenuation increased; using a lower-frequency call may increase the range of detection by a few meters as atmospheric attenuation increases. The same trend was not observed for FM calls, which may be explained by the primary role of these signals in short-range target localization.UCR::Sedes Regionales::Sede del Su

Comparing the efficiency of monofilament and traditional nets for capturing bats

Traditional mist nets used for capturing bats have several drawbacks, particularly that they are inefficient at sampling many insectivorous species. One possible alternative is to use monofilament nets, whose netting is made of single strands of yarn instead of several as regular nets, making them less detectable. To date, no study has quantified the capture efficiency of monofilament nets compared to regular mist nets for the study of bats. Here we compare capture efficiency of monofilament and regular mist nets, focusing on bat abundance and species diversity at a lowland tropical forest in southwestern Costa Rica. During our sampling period, we captured 90 individuals and 14 species in regular nets and 125 individuals and 20 species in monofilament nets. The use of monofilament nets increased overall capture rates, but most notably for insectivorous species. Species accumulation curves indicate that samples based on regular nets are significantly underestimating species diversity, most notably as these nets fail at sampling rare species. We show that incorporating monofilament nets into bat studies offers an opportunity to expand records of different guilds and rare bat species and to improve our understanding of poorly-known bat assemblages while using a popular, relatively cheap and portable sampling method.UCR::Sedes Regionales::Sede del SurUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Contrasting Thermal Strategies of Montane Neotropical Bats at High Elevations

In the Neotropics, captive vespertilionid bats substantially reduce their metabolic rate at low ambient temperatures, similar to their temperate counterparts, whereas the ability of phyllostomids to lower metabolic rate seems to be more limited, even in mountain species. Nevertheless, field data on the thermal behaviour of syntopic individuals from these two families is lacking. Consequently, we aimed to test whether torpor was more common and deeper in vesper bats compared to leaf-nosed bats by studying skin temperature (Tsk) variation in individuals experiencing the same environmental conditions at a mountain area. Bats experienced ambient temperatures below 15 °C. Average Tsk was 10 °C in Myotis oxyotus gardneri (Vespertilionidae) during the day, while Sturnira burtonlimi (Phyllostomidae) regulated diurnal Tsk above 30 °C. Constant food availability may explain why diurnal Sturnira burtonlimi pay the high energetic cost required to remain normothermic and to defend a wide Ta–Tsk gap but further studies are needed to elucidate additional strategies that may be employed by these bats to reduce the energetic demands of normothermy. Our study shows that the contrasting thermal strategies and torpor use adopted by vespertilionid insectivores and phyllostomid frugivores in captive settings also occur in free-ranging conditions, thus providing a basis to develop further studies with predictions more accurately rooted in field data.Government of the Basque Country/[IT754-13]//País BascoUCR::Sedes Regionales::Sede del SurUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Cave-dwelling fauna of Costa Rica: current state of knowledge and future research perspectives

This study focused on the cave fauna of Costa Rica, which has remained relatively understudied despite the presence of more than 435 recorded natural caves and artificial subterranean sites. We collected and reviewed all available literature data on cave fauna in Costa Rica and created the first comprehensive review of the existing information. In addition, we report new records from field surveys conducted between 2015 and 2018. This study reported approximately 123 animal species, whereas the remaining records (n = 82) represented taxa that could not be identified at the species level. Data were collected from 127 locations throughout the country, with new cave fauna records from 41 sites. Notably, we reported the first occurrence of the true bug Amnestus subferrugineus (Westwood 1837) within Costa Rican caves, which represents an addition to the country’s faunal inventory. As this study highlights the knowledge gaps in the subterranean fauna, it will serve as an important stepping stone for future research and conservation efforts related to caves in Costa Rica

Response of a Specialist Bat to the Loss of a Critical Resource

Human activities have negatively impacted many species, particularly those with unique traits that restrict their use of resources and conditions to specific habitats. Unfortunately, few studies have been able to isolate the individual and combined effects of different threats on population persistence in a natural setting, since not all organisms can be associated with discrete habitat features occurring over limited spatial scales. We present the results of a field study that examines the short-term effects of roost loss in a specialist bat using a conspicuous, easily modified resource. We mimicked roost loss in the natural habitat and monitored individuals before and after the perturbation to determine patterns of resource use, spatial movements, and group stability. Our study focused on the disc-winged bat Thyroptera tricolor, a species highly morphologically specialized for roosting in the developing furled leaves of members of the order Zingiberales. We found that the number of species used for roosting increased, that home range size increased (before: mean 0.14±SD 0.08 ha; after: 0.73±0.68 ha), and that mean association indices decreased (before: 0.95±0.10; after: 0.77±0.18) once the roosting habitat was removed. These results demonstrate that the removal of roosting resources is associated with a decrease in roost-site preferences or selectivity, an increase in mobility of individuals, and a decrease in social cohesion. These responses may reduce fitness by potentially increasing energetic expenditure, predator exposure, and a decrease in cooperative interactions. Despite these potential risks, individuals never used roost-sites other than developing furled leaves, suggesting an extreme specialization that could ultimately jeopardize the long-term persistence of this species' local populations

Comparative social network analysis in a leaf-roosting bat

Even though social network analysis provides an important tool to characterize and compare societies, no studies have used its analytical applications to characterize patterns of sociality in bats. Here I use social network analysis to characterize and compare patterns of sociality between three populations of the leaf-roosting bat Thyroptera tricolor. Sites differed in the density of furled leaves used by T. tricolor for roosting. Finca had more leaves per hectare (77), followed by Ureña (58), and Esquinas (7). The time period over which the probability of association is halved based on fitted models was 1,086 days for Finca, 714 days for Ureña, and 303 days for Esquinas. Finca and Ureña had very similar network topologies, with several small clusters, high-clustering coefficients, short path lengths, low node betweenness, and high network robustness. Social networks at Esquinas were composed of one large cluster and several small isolated ones. Esquinas also had high-clustering coefficients, but path length and node betweenness were high. Network resilience was lower in Esquinas compared to Finca and Ureña. These results show that, unlike many other forest-dwelling bats that switch roosts regularly, T. tricolor does not exhibit a typical fission–fusion social system, and that resource availability seems to affect social networks in this bat. In addition, this study highlights the importance of emigrating individuals in maintaining social cohesion, establishing network connectedness, and determining network robustness.UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí