Reavaliação da produção de aflatoxina produzida por isolados de Aspergillus candidus e Eurotium chevalieri de rações para aves no Brasil

Commercial feedstuffs in Brazil are an important component in modern animal husbandry. Eurotium chevalieri and Aspergillus candidus have been reported as poultry feed contaminants in Brazil, however, their potential  ability to produce aflatoxins on this substrate has not been evaluated. Therefore, the aim of this work was to evaluate the aflatoxin-producing ability by A. candidus and E. chevalieri strains isolated from poultry feed and raw materials in Brazil. Mycological analysis was done to isolate total mycobiota. Aspergillus candidus and E. chevalieri strains were sub-cultured in yeast extract sucrose for aflatoxin production. TLC and HPLC analyses were done to demonstrate the aflatoxins production. Aspergillus candidus was isolated at 7.56 and 8.19% from poultry feed and raw materials, respectively, while 26.05 and 26.33% of E. chevalieri strains were isolated. They were able to produce aflatoxin B1, B2, G1 and G2. Eurotium chevalieri produced higher amounts of aflatoxin B1 and G1 than A. candidus. AFG1 and AFB1 production were the higher produced aflatoxins. The potential production of aflatoxins by these fungi could be an unexpected hazard to animal health when poultry feed is consumed. The toxigenic ability knowledgement by these species is important to guarantee the quality of animal feeds. Eurotium chevalieri and Aspergillus candidus have been reported as poultry feed contaminants in Brazil, however, their potential  ability to produce aflatoxins on this substrate has not been evaluated. Therefore, the aim of this work was to evaluate the aflatoxin-producing ability by A. candidus and E. chevalieri strains isolated from poultry feed and raw materials in Brazil. Mycological analysis was done to isolate total mycobiota. Aspergillus candidus and E. chevalieri strains were sub-cultured in yeast extract sucrose for aflatoxin production. TLC and HPLC analyses were done to demonstrate the aflatoxins production. Aspergillus candidus was isolated at 7.56 and 8.19% from poultry feed and raw materials, respectively, while 26.05 and 26.33% of E. chevalieri strains were isolated. They were able to produce aflatoxin B1, B2, G1 and G2. Eurotium chevalieri produced higher amounts of aflatoxin B1 and G1 than A. candidus. AFG1 and AFB1 production were the higher produced aflatoxins. The potential production of aflatoxins by these fungi could be an unexpected hazard to animal health when poultry feed is consumed. The toxigenic ability knowledgement by these species is important to guarantee the quality of animal feeds.Ração comercial no Brasil é um componente importante na produção animal moderna. Eurotium chevalieri e Aspergillus candidus são isolados como contaminantes em ração de aves no Brasil, porém, o potencial desses fungos em produzir aflatoxinas neste substrato não é avaliado. Entretanto, a objetivo deste trabalho foi avaliar a capacidade toxígena de A. candidus e E. chevalieri isolado de alimento de aves e matérias primas no Brasil. Análise micológica foi realizada para isolar micobiota total. Aspergillus candidus e E. chevalieri foram inoculados em meio sacarose e extrato de levedura para produção de aflatoxinas. As análises foram realizadas em CCD e CLAE para demonstrar a produção das aflatoxinas. Aspergillus candidus foi isolado em 7,56 e 8,19% de ração para aves e matérias-primas, respectivamente. Enquanto 26,05 e 26,33% de E. chevalieri foi isolados de ração para aves e matérias-primas, respectivamente. Esses fungos produziram aflatoxinas B1, B2, G1 e G2. Eurotium chevalieri produziu quantidades mais altas de aflatoxinas B1 e G1 que A. candidus. As AFG1 e AFB1 foram as que apresentaram maior produção. Os potenciais de produção de aflatoxinas por estes fungos apresentam um perigo à saúde animal quando consumidas. O conhecimento da capacidade toxígena destas espécies é importante para garantir a qualidade da alimentação animal.Fil: Fraga, Marcelo Elías. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Direito, Gloria Maria. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Gatti, Mario Jorge. Instituto Oswaldo Cruz; BrasilFil: Moraes, Áurea Maria Lage. Instituto Oswaldo Cruz; BrasilFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: da Rocha Rosa, Carlos Alberto. Universidade Federal Rural do Rio de Janeiro; Brasi

Characterization of Aspergillus species based on fatty acid profiles

Submitted by Sandra Infurna ([email protected]) on 2018-07-10T12:22:53Z No. of bitstreams: 1 marioj_gatti_etal_IOC_2008.pdf: 340819 bytes, checksum: 0b1e15fe67438cea2db176e1aef202e5 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-07-10T12:40:21Z (GMT) No. of bitstreams: 1 marioj_gatti_etal_IOC_2008.pdf: 340819 bytes, checksum: 0b1e15fe67438cea2db176e1aef202e5 (MD5)Made available in DSpace on 2018-07-10T12:40:21Z (GMT). No. of bitstreams: 1 marioj_gatti_etal_IOC_2008.pdf: 340819 bytes, checksum: 0b1e15fe67438cea2db176e1aef202e5 (MD5) Previous issue date: 2008Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Tecnologia de Alimentos. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Rio Cuarto, Córodba, Argentina.Universidade Federal Rural do Rio de Janeiro. Departamento de Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Cellular fatty acid (FA) composition was utilized as a taxonomic tool to discriminate between different Aspergillus species. Several of the tested species had the same FA composition and different relative FA concentrations. The most important FAs were palmitic acid (C16:0), estearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2), which represented 95% of Aspergillus FAs. Multivariate data analysis demonstrated that FA analysis is a useful tool for differentiating species belonging to genus Aspergillus. All the species analyzed showed significantly FA acid profiles (p < 0.001). Furthermore, it will be possible to distinguish among Aspergillus spp. in the Flavi Section. FA composition can serve as a useful tool for the identification of filamentous fungi

Mycobiota in poultry feeds and natural occurrence of aflatoxins, fumonisins and zearalenone in the Rio de Janeiro State, Brazil

The intake of mycotoxin-contaminated feeds can lead to nutrient losses and may have adverse effects on animal health and on productivity. The aims of this study were 1) to determine the mycobiota present in poultry feed samples, and 2) to evaluate the natural occurrence of aflatoxin B1, fumonisin B1 and zearalenone. Fungal counts were similar between all culture media tested (103 CFU g-1). The most frequent genus isolated was Penicillium spp. (41.26%) followed by Aspergillus spp. (33.33%) and Fusarium spp. (20.63%). High precision liquid chromatography was applied to quantify aflatoxin B1 and fumonisin B1. Thin layer chromatography was used to determine zearalenone levels. Aflatoxin B1 values ranged between 1.2 and 17.5 µg kg-1. Fumonisin B1 levels ranged between 1.5 and 5.5 mg g-1. Zearalenone levels ranged between 0.1 and 7 mg g-1. The present study shows the simultaneous occurrence of two carcinogenic mycotoxins, aflatoxin B1 and fumonisin B1, together with another Fusarium mycotoxin (zearalenone) in feed intended for poultry consumption. Many samples contained AFB1 levels near the permissible maximum and it could affect young animals. A synergistic toxic response is possible in animals under simultaneous exposure.Fil: Oliveira, Glenda R.. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Ribeiro, Jessika M.. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Fraga, Marcelo. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Direito, Gloria M.. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Keller, Kelly Moura. Universidade Federal Rural do Rio de Janeiro; BrasilFil: Dalcero, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Rosa, Carlos A.. Universidade Federal Rural do Rio de Janeiro; Brasi