Thyrotropin (TSH), free thyroxine (fT4) and thyroperoxidase (TPO) antibodies among women with and without gestational diabetes (GDM).

<p>Thyrotropin (TSH), free thyroxine (fT4) and thyroperoxidase (TPO) antibodies among women with and without gestational diabetes (GDM).</p

Schematic diagram depicting how caloric intake and deiodinase activity fit into relationships shown in Fig 2.

<p>Higher caloric intake (C) reflects higher weight (A) and induces higher deiodinase activity (D) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149065#pone.0149065.ref032" target="_blank">32</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149065#pone.0149065.ref034" target="_blank">34</a>]. Lower fT4 (E) and higher T3/T4 ratios (F) occur as a consequence of higher deiodinase activity and are associated with both insulin resistance (G) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149065#pone.0149065.ref024" target="_blank">24</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0149065#pone.0149065.ref025" target="_blank">25</a>] and gestational diabetes (B).</p

Extent to which variables that differ significantly between women with and without gestational diabetes might influence fT4 concentration (9,079 women without gestational diabetes).

<p>Extent to which variables that differ significantly between women with and without gestational diabetes might influence fT4 concentration (9,079 women without gestational diabetes).</p

Characteristics of women with and without gestational diabetes.

<p>Characteristics of women with and without gestational diabetes.</p

Relationships between gestational diabetes (GDM) and age (Fig 1A), weight (Fig 1B), free thyroxine (fT4) (Fig 1C), and thyrotropin (TSH) (Fig 1D).

<p>Open circles represent percents of GDM cases at each decile of the variable shown on the X-axis. Solid lines indicate unadjusted slopes of the respective relationships; p-values indicate slope significance. The dotted line in Fig 1C shows the slope of the fT4/GDM relationship, after adjustment for age, weight, and TSH.</p

Summary of sample types utilized for each of the studies performed.

<p>Summary of sample types utilized for each of the studies performed.</p

Summary of analysis results for each of the studies performed. Sens = sensitivity.

<p>Spec = specificity; NA = Not applicable.</p

Paired comparison of z-scores.

<p>Z-scores were calculated for paired samples with previously described GC normalized, repeat masked z-scores on the x-axis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057381#pone.0057381-Palomaki1" target="_blank">[7]</a> and z-scores from the same libraries sequenced in 12-plex on the y-axis. Samples classified by karyotype analysis as trisomies for A) Chromosome 21, B) Chromosome 13, or C) Chromosome 18 are shown in blue; unaffected samples for each aneuploidy condition are shown in gray. Red horizontal and vertical lines in each plot represent the respective classification cutoff for that chromosome (z = 3 for chromosome 21, z = 3.95 for chromosomes 13 and 18).</p

Paired comparison of z-scores.

<p>Z-scores were calculated for 1269 paired samples with previously described GC normalized, repeat masked z-scores on the x-axis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057381#pone.0057381-Palomaki1" target="_blank">[7]</a> and z-scores from the high-throughput assay on the y-axis. Samples classified by karyotype analysis as trisomies for A) Chromsome 21, B) Chromosome 13, or C) Chromosome 18 are shown in blue; unaffected samples for each aneuploidy condition are shown in gray. Red horizontal and vertical lines in each plot represent the respective classification cutoff for that chromosome (z = 3 for chromosome 21, z = 3.95 for chromosomes 13 and 18). Black line in plot represents y = x.</p