Lipid Antioxidant and Galactolipid Remodeling under Temperature Stress in Tomato Plants

Increased temperatures are a major scenario in climate change and present a threat to plant growth and agriculture. Plant growth depends on photosynthesis. To function optimally, the photosynthetic machinery at the thylakoid membrane in chloroplasts continuously adapts to changing conditions. Here, we set out to discover the most important changes arising at the lipid level under high temperature (38°C) in comparison to mild (20°C) and moderately cold temperature (10°C) using a non- targeted lipidomics approach. To our knowledge, no comparable experiment at the level of the whole membrane system has been documented. Here, 791 molecular species were detected by mass spectrometry and ranged from membrane lipids, prenylquinones (tocopherols, phylloquinone, plastoquinone, plastochromanol), carotenoids (ß-carotene, xanthophylls) to numerous unidentified compounds. At high temperatures, the most striking changes were observed for the prenylquinones (a- tocopherol and plastoquinone/-ol) and the degree of saturation of fatty acids in galactolipids and phosphatidyl ethanolamine. Photosynthetic efficiency at high temperature was not affected but at moderately cold temperature mild photoinhibition occurred. The results indicate, that the thylakoid membrane is remodeled with regard to fatty acid saturation in galactolipids and lipid antioxidant concentrations under high temperature stress. The data strongly suggest, that massively increased concentrations of a-tocopherol and plastoquinone are important for protection against high temperature stress and proper function of the photosynthetic apparatus

Trade-off between constitutive and inducible resistance against herbivores is only partially explained by gene expression and glucosinolate production

The observed partial correlation between herbivore resistance, defensive metabolites accumulation, and gene expression suggests a complex network of gene interactions governing the postulated trade-off between constitutive defences and their inducibilit

Fertilization with beneficial microorganisms decreases tomato defenses against insect pests

The adverse effects of chemical fertilizers on agricultural fields and the environment are compelling society to move toward more sustainable farming techniques. "Effective microorganisms” is a beneficial microbial mixture that has been developed to improve soil quality and crop yield while simultaneously dramatically reducing organic chemical application. Additional indirect benefits of beneficial microorganisms application may include increased plant resistance to herbivore attack, though this has never been tested till now. Tomato plants were grown in controlled greenhouse conditions in a full-factorial design with beneficial microorganisms inoculation and commercial chemical fertilizer application as main factors. We measured plant yield and growth parameters, as well as resistance against the generalist pest Spodoptera littoralis moth larval attack. Additionally, we measured plant defensive chemistry to underpin resistance mechanisms. Overall, we found that, comparable to chemical fertilizer, beneficial microorganisms increased plant growth fruit production by 35 and 61%, respectively. Contrary to expectations, plants inoculated with beneficial microorganisms sustained 25% higher insect survival and larvae were in average 41% heavier than on unfertilized plants. We explain these results by showing that beneficial microorganism-inoculated plants were impaired in the induction of the toxic glycoalkaloid molecule tomatine and the defense-related phytohormone jasmonic acid after herbivore attack. For the first time, we therefore show that biofertilizer application might endure unintended, pest-mediated negative effects, and we thus suggest that biofertilizer companies should incorporate protection attributes in their studies prior to commercialization

Identification of Plastoglobules as a Site of Carotenoid Cleavage

Carotenoids play an essential role in light harvesting and protection from excess light. During chloroplast senescence carotenoids are released from their binding proteins and are eventually metabolized. Carotenoid cleavage dioxygenase 4 (CCD4) is involved in carotenoid breakdown in senescing leaf and desiccating seed, and is part of the proteome of plastoglobules (PG), which are thylakoid-associated lipid droplets. Here, we demonstrate that CCD4 is functionally active in PG. Leaves of Arabidopsis thaliana ccd4 mutants constitutively expressing CCD4 fused to yellow fluorescent protein showed strong fluorescence in PG and reduced carotenoid levels upon dark- induced senescence. Lipidome-wide analysis indicated that ß-carotene, lutein, and violaxanthin were the principle substrates of CCD4 in vivo and were cleaved in senescing chloroplasts. Moreover, carotenoids were shown to accumulate in PG of ccd4 mutant plants during senescence, indicating translocation of carotenoids to PG prior to degradation

Contamination by neonicotinoid insecticides in Barn owls (Tyto alba) and Alpine swifts (Tachymarptis melba)

Acknowledgements We thank all the students and volunteers who helped us in their fields. This study was supported by a Swiss National Science Foundation overheads grant, a grant from the Federal Office for the Environment and an SNSF-förderungs professor grant to FH. Photographs used in the graphical abstract are copyrights of Alexandre Roulin, Pierre Bize and Shirley Laurent. The authors bear sole responsibility for the content and declare no conflict of interest.Peer reviewedPublisher PD